Chromene stability: The most potent estrogenic compounds in White Kwao Krua (Pueraria candollei var mirifica) crude extract
Pueraria candollei var. mirifica (PM) is a phytoestrogen-rich herb with rejuvenating qualities. Stability of chromenes, comprising deoxymiroestrol, miroestrol and isomiroestrol, in crude extract of PM was investigated. The effects of acid-base condition and temperature on stability of the chromene s...
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doaj-c4efd0a9e1c8458e9f9342f9789e32e22021-04-29T04:45:23ZengElsevierJournal of Functional Foods1756-46462015-12-0119269277Chromene stability: The most potent estrogenic compounds in White Kwao Krua (Pueraria candollei var mirifica) crude extractOrapin Udomsin0Thaweesak Juengwatanatrakul1Gorawit Yusakul2Waraporn Putalun3Faculty of Pharmaceutical Sciences, Khon Kaen University, 40002 Khon Kaen, Thailand; Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, 40002 Khon Kaen, ThailandFaculty of Pharmaceutical Sciences, Ubon Ratchathani University, 34190 Ubon Ratchathani, ThailandGraduate School of Pharmaceutical Sciences, Kyushu University, 8128582 Fukuoka, JapanFaculty of Pharmaceutical Sciences, Khon Kaen University, 40002 Khon Kaen, Thailand; Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, 40002 Khon Kaen, Thailand; Corresponding author. Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand. Tel.: +66 43 362095; fax: +66 43 202379.Pueraria candollei var. mirifica (PM) is a phytoestrogen-rich herb with rejuvenating qualities. Stability of chromenes, comprising deoxymiroestrol, miroestrol and isomiroestrol, in crude extract of PM was investigated. The effects of acid-base condition and temperature on stability of the chromene standard solution and the PM crude extract were analysed using gradient HPLC. The degradation of the chromenes in the extract was sensitive to high temperatures and strongly acidic or alkaline solutions. Specifically, deoxymiroestrol, which exhibited the highest estrogenic activity, can be easy degraded to miroestrol or isomiroestrol. However, chromene standard solution was stable in ethanol after storage for 360 days at 25 °C. The crude extract of PM in the solid form was stable when stored at −20 or 4 °C for 360 days. Therefore, the extract could be stored in the solid form at low temperatures. This method provides a system to simultaneously identify chromenes in crude extract of PM.http://www.sciencedirect.com/science/article/pii/S1756464615004533HPLC (high-performance liquid chromatography)StabilityDeoxymiroestrolMiroestrolIsomiroestrolPueraria candollei var. mirifica crude extract |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Orapin Udomsin Thaweesak Juengwatanatrakul Gorawit Yusakul Waraporn Putalun |
spellingShingle |
Orapin Udomsin Thaweesak Juengwatanatrakul Gorawit Yusakul Waraporn Putalun Chromene stability: The most potent estrogenic compounds in White Kwao Krua (Pueraria candollei var mirifica) crude extract Journal of Functional Foods HPLC (high-performance liquid chromatography) Stability Deoxymiroestrol Miroestrol Isomiroestrol Pueraria candollei var. mirifica crude extract |
author_facet |
Orapin Udomsin Thaweesak Juengwatanatrakul Gorawit Yusakul Waraporn Putalun |
author_sort |
Orapin Udomsin |
title |
Chromene stability: The most potent estrogenic compounds in White Kwao Krua (Pueraria candollei var mirifica) crude extract |
title_short |
Chromene stability: The most potent estrogenic compounds in White Kwao Krua (Pueraria candollei var mirifica) crude extract |
title_full |
Chromene stability: The most potent estrogenic compounds in White Kwao Krua (Pueraria candollei var mirifica) crude extract |
title_fullStr |
Chromene stability: The most potent estrogenic compounds in White Kwao Krua (Pueraria candollei var mirifica) crude extract |
title_full_unstemmed |
Chromene stability: The most potent estrogenic compounds in White Kwao Krua (Pueraria candollei var mirifica) crude extract |
title_sort |
chromene stability: the most potent estrogenic compounds in white kwao krua (pueraria candollei var mirifica) crude extract |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2015-12-01 |
description |
Pueraria candollei var. mirifica (PM) is a phytoestrogen-rich herb with rejuvenating qualities. Stability of chromenes, comprising deoxymiroestrol, miroestrol and isomiroestrol, in crude extract of PM was investigated. The effects of acid-base condition and temperature on stability of the chromene standard solution and the PM crude extract were analysed using gradient HPLC. The degradation of the chromenes in the extract was sensitive to high temperatures and strongly acidic or alkaline solutions. Specifically, deoxymiroestrol, which exhibited the highest estrogenic activity, can be easy degraded to miroestrol or isomiroestrol. However, chromene standard solution was stable in ethanol after storage for 360 days at 25 °C. The crude extract of PM in the solid form was stable when stored at −20 or 4 °C for 360 days. Therefore, the extract could be stored in the solid form at low temperatures. This method provides a system to simultaneously identify chromenes in crude extract of PM. |
topic |
HPLC (high-performance liquid chromatography) Stability Deoxymiroestrol Miroestrol Isomiroestrol Pueraria candollei var. mirifica crude extract |
url |
http://www.sciencedirect.com/science/article/pii/S1756464615004533 |
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