Species-Dependent Functionality of the Human Cytolytic Fusion Proteins Granzyme B-H22(scFv) and H22(scFv)-Angiogenin in Macrophages
Human cytolytic fusion proteins (hCFPs) are comprised of a specific cell-surface-binding moiety and an effector molecule of human origin. In contrast to common immunotoxins, including bacterial or plant toxins, they are considered not to be immunogenic. Two examples for human pro-apoptotic effector...
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doaj-c4fc28ca06f54d36aa55e814992f431c2020-11-24T20:57:12ZengMDPI AGAntibodies2073-44682013-01-012191810.3390/antib2010009Species-Dependent Functionality of the Human Cytolytic Fusion Proteins Granzyme B-H22(scFv) and H22(scFv)-Angiogenin in MacrophagesTheo ThepenStefan BarthRainer FischerMichael HuhnRadoslav MladenovEric AslanianDmitrij HristodorovSonja SchifferHuman cytolytic fusion proteins (hCFPs) are comprised of a specific cell-surface-binding moiety and an effector molecule of human origin. In contrast to common immunotoxins, including bacterial or plant toxins, they are considered not to be immunogenic. Two examples for human pro-apoptotic effector proteins are the serine protease Granzyme B and the RNase Angiogenin. Pre-clinical testing of functionality in in vitro and in vivo studies is essential for therapeutics. Establishing relevant animal models that have predictive value for therapeutic success is a great challenge in biomedical research. In this study, we investigated the species-dependent cytotoxic activity of two hCFPs prior to their application in a murine inflammation model. We found that in vitro and ex vivo either hCFP was able to kill human cells only, leaving murine cells unaffected. In contrast, no species-dependency was found for the bacterial Pseudomonas exotoxin A based immunotoxin H22(scFv)-ETA’. This species-dependent functioning has to be carefully considered when performing pre-clinical studies in animal models.http://www.mdpi.com/2073-4468/2/1/9immunotoxinCD64inflammationmouse model |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Theo Thepen Stefan Barth Rainer Fischer Michael Huhn Radoslav Mladenov Eric Aslanian Dmitrij Hristodorov Sonja Schiffer |
spellingShingle |
Theo Thepen Stefan Barth Rainer Fischer Michael Huhn Radoslav Mladenov Eric Aslanian Dmitrij Hristodorov Sonja Schiffer Species-Dependent Functionality of the Human Cytolytic Fusion Proteins Granzyme B-H22(scFv) and H22(scFv)-Angiogenin in Macrophages Antibodies immunotoxin CD64 inflammation mouse model |
author_facet |
Theo Thepen Stefan Barth Rainer Fischer Michael Huhn Radoslav Mladenov Eric Aslanian Dmitrij Hristodorov Sonja Schiffer |
author_sort |
Theo Thepen |
title |
Species-Dependent Functionality of the Human Cytolytic Fusion Proteins Granzyme B-H22(scFv) and H22(scFv)-Angiogenin in Macrophages |
title_short |
Species-Dependent Functionality of the Human Cytolytic Fusion Proteins Granzyme B-H22(scFv) and H22(scFv)-Angiogenin in Macrophages |
title_full |
Species-Dependent Functionality of the Human Cytolytic Fusion Proteins Granzyme B-H22(scFv) and H22(scFv)-Angiogenin in Macrophages |
title_fullStr |
Species-Dependent Functionality of the Human Cytolytic Fusion Proteins Granzyme B-H22(scFv) and H22(scFv)-Angiogenin in Macrophages |
title_full_unstemmed |
Species-Dependent Functionality of the Human Cytolytic Fusion Proteins Granzyme B-H22(scFv) and H22(scFv)-Angiogenin in Macrophages |
title_sort |
species-dependent functionality of the human cytolytic fusion proteins granzyme b-h22(scfv) and h22(scfv)-angiogenin in macrophages |
publisher |
MDPI AG |
series |
Antibodies |
issn |
2073-4468 |
publishDate |
2013-01-01 |
description |
Human cytolytic fusion proteins (hCFPs) are comprised of a specific cell-surface-binding moiety and an effector molecule of human origin. In contrast to common immunotoxins, including bacterial or plant toxins, they are considered not to be immunogenic. Two examples for human pro-apoptotic effector proteins are the serine protease Granzyme B and the RNase Angiogenin. Pre-clinical testing of functionality in in vitro and in vivo studies is essential for therapeutics. Establishing relevant animal models that have predictive value for therapeutic success is a great challenge in biomedical research. In this study, we investigated the species-dependent cytotoxic activity of two hCFPs prior to their application in a murine inflammation model. We found that in vitro and ex vivo either hCFP was able to kill human cells only, leaving murine cells unaffected. In contrast, no species-dependency was found for the bacterial Pseudomonas exotoxin A based immunotoxin H22(scFv)-ETA’. This species-dependent functioning has to be carefully considered when performing pre-clinical studies in animal models. |
topic |
immunotoxin CD64 inflammation mouse model |
url |
http://www.mdpi.com/2073-4468/2/1/9 |
work_keys_str_mv |
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