Coupled Gas-Exchange Model for C₄ Leaves Comparing Stomatal Conductance Models

• Main Authors: Kyungdahm Yun, Dennis Timlin, Soo-Hyung Kim
• Format: Article
• Language: English
• Published: MDPI AG 2020-10-01
• Series: Plants
• Subjects: gas-exchange; C₄ photosynthesis; stomatal conductance; Ball–Berry; Medlyn
• Online Access: https://www.mdpi.com/2223-7747/9/10/1358

Plant simulation models are abstractions of plant physiological processes that are useful for investigating the responses of plants to changes in the environment. Because photosynthesis and transpiration are fundamental processes that drive plant growth and water relations, a leaf gas-exchange model that couples their interdependent relationship through stomatal control is a prerequisite for explanatory plant simulation models. Here, we present a coupled gas-exchange model for <inline-formula><math display="inline"><semantics><msub><mi>C</mi><mn>4</mn></msub></semantics></math></inline-formula> leaves incorporating two widely used stomatal conductance submodels: Ball–Berry and Medlyn models. The output variables of the model includes steady-state values of <inline-formula><math display="inline"><semantics><mrow><mi>C</mi><msub><mi>O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula> assimilation rate, transpiration rate, stomatal conductance, leaf temperature, internal <inline-formula><math display="inline"><semantics><mrow><mi>C</mi><msub><mi>O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula> concentrations, and other leaf gas-exchange attributes in response to light, temperature, <inline-formula><math display="inline"><semantics><mrow><mi>C</mi><msub><mi>O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula>, humidity, leaf nitrogen, and leaf water status. We test the model behavior and sensitivity, and discuss its applications and limitations. The model was implemented in Julia programming language using a novel modeling framework. Our testing and analyses indicate that the model behavior is reasonably sensitive and reliable in a wide range of environmental conditions. The behavior of the two model variants differing in stomatal conductance submodels deviated substantially from each other in low humidity conditions. The model was capable of replicating the behavior of transgenic <inline-formula><math display="inline"><semantics><msub><mi>C</mi><mn>4</mn></msub></semantics></math></inline-formula> leaves under moderate temperatures as found in the literature. The coupled model, however, underestimated stomatal conductance in very high temperatures. This is likely an inherent limitation of the coupling approaches using Ball–Berry type models in which photosynthesis and stomatal conductance are recursively linked as an input of the other.