A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area
Abstract Data from recent studies support the hypothesis that infections by human gastrointestinal (GI) helminths impact, directly and/or indirectly, on the composition of the host gut microbial flora. However, to the best of our knowledge, these studies have been conducted in helminth-endemic areas...
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doaj-c532b10f3103458694357cf986f8996e2020-12-08T03:32:07ZengNature Publishing GroupScientific Reports2045-23222018-10-018111310.1038/s41598-018-33937-3A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic areaTimothy P. Jenkins0Fabio Formenti1Cecilia Castro2Chiara Piubelli3Francesca Perandin4Dora Buonfrate5Domenico Otranto6Julian L. Griffin7Lutz Krause8Zeno Bisoffi9Cinzia Cantacessi10Department of Veterinary Medicine, University of CambridgeCentre for Tropical Diseases, IRCCS Sacro Cuore-Don Calabria Hospital, NegrarDepartment of Biochemistry, University of CambridgeCentre for Tropical Diseases, IRCCS Sacro Cuore-Don Calabria Hospital, NegrarCentre for Tropical Diseases, IRCCS Sacro Cuore-Don Calabria Hospital, NegrarCentre for Tropical Diseases, IRCCS Sacro Cuore-Don Calabria Hospital, NegrarDepartment of Veterinary Medicine, University of BariDepartment of Biochemistry, University of CambridgeThe University of Queensland Diamantina Institute, Translational Research InstituteCentre for Tropical Diseases, IRCCS Sacro Cuore-Don Calabria Hospital, NegrarDepartment of Veterinary Medicine, University of CambridgeAbstract Data from recent studies support the hypothesis that infections by human gastrointestinal (GI) helminths impact, directly and/or indirectly, on the composition of the host gut microbial flora. However, to the best of our knowledge, these studies have been conducted in helminth-endemic areas with multi-helminth infections and/or in volunteers with underlying gut disorders. Therefore, in this study, we explore the impact of natural mono-infections by the human parasite Strongyloides stercoralis on the faecal microbiota and metabolic profiles of a cohort of human volunteers from a non-endemic area of northern Italy (S+), pre- and post-anthelmintic treatment, and compare the findings with data obtained from a cohort of uninfected controls from the same geographical area (S−). Analyses of bacterial 16S rRNA high-throughput sequencing data revealed increased microbial alpha diversity and decreased beta diversity in the faecal microbial profiles of S+ subjects compared to S−. Furthermore, significant differences in the abundance of several bacterial taxa were observed between samples from S+ and S− subjects, and between S+ samples collected pre- and post-anthelmintic treatment. Faecal metabolite analysis detected marked increases in the abundance of selected amino acids in S+ subjects, and of short chain fatty acids in S− subjects. Overall, our work adds valuable knowledge to current understanding of parasite-microbiota associations and will assist future mechanistic studies aimed to unravel the causality of these relationships.https://doi.org/10.1038/s41598-018-33937-3Strongyloides StercoralisStercoralis InfectionMicrobial Alpha DiversityDecreased Beta DiversityFecal Metabolome |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Timothy P. Jenkins Fabio Formenti Cecilia Castro Chiara Piubelli Francesca Perandin Dora Buonfrate Domenico Otranto Julian L. Griffin Lutz Krause Zeno Bisoffi Cinzia Cantacessi |
spellingShingle |
Timothy P. Jenkins Fabio Formenti Cecilia Castro Chiara Piubelli Francesca Perandin Dora Buonfrate Domenico Otranto Julian L. Griffin Lutz Krause Zeno Bisoffi Cinzia Cantacessi A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area Scientific Reports Strongyloides Stercoralis Stercoralis Infection Microbial Alpha Diversity Decreased Beta Diversity Fecal Metabolome |
author_facet |
Timothy P. Jenkins Fabio Formenti Cecilia Castro Chiara Piubelli Francesca Perandin Dora Buonfrate Domenico Otranto Julian L. Griffin Lutz Krause Zeno Bisoffi Cinzia Cantacessi |
author_sort |
Timothy P. Jenkins |
title |
A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area |
title_short |
A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area |
title_full |
A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area |
title_fullStr |
A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area |
title_full_unstemmed |
A comprehensive analysis of the faecal microbiome and metabolome of Strongyloides stercoralis infected volunteers from a non-endemic area |
title_sort |
comprehensive analysis of the faecal microbiome and metabolome of strongyloides stercoralis infected volunteers from a non-endemic area |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2018-10-01 |
description |
Abstract Data from recent studies support the hypothesis that infections by human gastrointestinal (GI) helminths impact, directly and/or indirectly, on the composition of the host gut microbial flora. However, to the best of our knowledge, these studies have been conducted in helminth-endemic areas with multi-helminth infections and/or in volunteers with underlying gut disorders. Therefore, in this study, we explore the impact of natural mono-infections by the human parasite Strongyloides stercoralis on the faecal microbiota and metabolic profiles of a cohort of human volunteers from a non-endemic area of northern Italy (S+), pre- and post-anthelmintic treatment, and compare the findings with data obtained from a cohort of uninfected controls from the same geographical area (S−). Analyses of bacterial 16S rRNA high-throughput sequencing data revealed increased microbial alpha diversity and decreased beta diversity in the faecal microbial profiles of S+ subjects compared to S−. Furthermore, significant differences in the abundance of several bacterial taxa were observed between samples from S+ and S− subjects, and between S+ samples collected pre- and post-anthelmintic treatment. Faecal metabolite analysis detected marked increases in the abundance of selected amino acids in S+ subjects, and of short chain fatty acids in S− subjects. Overall, our work adds valuable knowledge to current understanding of parasite-microbiota associations and will assist future mechanistic studies aimed to unravel the causality of these relationships. |
topic |
Strongyloides Stercoralis Stercoralis Infection Microbial Alpha Diversity Decreased Beta Diversity Fecal Metabolome |
url |
https://doi.org/10.1038/s41598-018-33937-3 |
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