Production, medium optimization, and structural characterization of an extracellular polysaccharide produced by Rhodotorula minuta ATCC 10658
Abstract Several strains of microorganism are capable of converting carbohydrates into extracellular polysaccharide. The preset research is a first effort made to optimize extracellular polysaccharide (CRMEP) by Rhodotorula minuta ATCC 10658 using one factor at time and response surface methods. One...
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Online Access: | https://doi.org/10.1002/fsn3.1792 |
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doaj-c5481a2ff6e1434188a9571bb2bc9ed82020-11-25T03:37:47ZengWileyFood Science & Nutrition2048-71772020-09-01894957496410.1002/fsn3.1792Production, medium optimization, and structural characterization of an extracellular polysaccharide produced by Rhodotorula minuta ATCC 10658Hamid Reza Samadlouie0Kambiz Jahanbin1Parisa jalali2Department of Food Science and Technology Faculty of Agriculture Shahrood University of Technology Shahrood IranDepartment of Food Science and Technology Faculty of Agriculture Shahrood University of Technology Shahrood IranDepartment of Food Science and Technology Faculty of Agriculture Shahrood University of Technology Shahrood IranAbstract Several strains of microorganism are capable of converting carbohydrates into extracellular polysaccharide. The preset research is a first effort made to optimize extracellular polysaccharide (CRMEP) by Rhodotorula minuta ATCC 10658 using one factor at time and response surface methods. One factor at time was applied in the initial screening of substrates prior to optimization study. Of all the substrates examined, starch as carbon source and defatted soy bean powder as protein source were discovered to be best for CRMEP production. Response surface analysis revealed that 15 g/L starch and 30g/L defatted soy bean powder were the optimal chemical conditions. The model predicted 13.22 g/L for CRMEP, which went along with the experimentally observed result. Purification of CRMEP by anion‐exchange column of DEAE‐cellulose yielded RMEP. Structural investigation indicated that the main chain of RMEP was composed of (1 → 3) and (1 → 4)‐linked mannopyranosyl residues, with branches attached to O‐6 of some (1 → 3)‐linked mannopyranosyl residues. The branches were composed of Glcp‐(1 → residues.https://doi.org/10.1002/fsn3.1792extracellular polysaccharideresponse surface methodologyRhodotorula minutastructural study |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hamid Reza Samadlouie Kambiz Jahanbin Parisa jalali |
spellingShingle |
Hamid Reza Samadlouie Kambiz Jahanbin Parisa jalali Production, medium optimization, and structural characterization of an extracellular polysaccharide produced by Rhodotorula minuta ATCC 10658 Food Science & Nutrition extracellular polysaccharide response surface methodology Rhodotorula minuta structural study |
author_facet |
Hamid Reza Samadlouie Kambiz Jahanbin Parisa jalali |
author_sort |
Hamid Reza Samadlouie |
title |
Production, medium optimization, and structural characterization of an extracellular polysaccharide produced by Rhodotorula minuta ATCC 10658 |
title_short |
Production, medium optimization, and structural characterization of an extracellular polysaccharide produced by Rhodotorula minuta ATCC 10658 |
title_full |
Production, medium optimization, and structural characterization of an extracellular polysaccharide produced by Rhodotorula minuta ATCC 10658 |
title_fullStr |
Production, medium optimization, and structural characterization of an extracellular polysaccharide produced by Rhodotorula minuta ATCC 10658 |
title_full_unstemmed |
Production, medium optimization, and structural characterization of an extracellular polysaccharide produced by Rhodotorula minuta ATCC 10658 |
title_sort |
production, medium optimization, and structural characterization of an extracellular polysaccharide produced by rhodotorula minuta atcc 10658 |
publisher |
Wiley |
series |
Food Science & Nutrition |
issn |
2048-7177 |
publishDate |
2020-09-01 |
description |
Abstract Several strains of microorganism are capable of converting carbohydrates into extracellular polysaccharide. The preset research is a first effort made to optimize extracellular polysaccharide (CRMEP) by Rhodotorula minuta ATCC 10658 using one factor at time and response surface methods. One factor at time was applied in the initial screening of substrates prior to optimization study. Of all the substrates examined, starch as carbon source and defatted soy bean powder as protein source were discovered to be best for CRMEP production. Response surface analysis revealed that 15 g/L starch and 30g/L defatted soy bean powder were the optimal chemical conditions. The model predicted 13.22 g/L for CRMEP, which went along with the experimentally observed result. Purification of CRMEP by anion‐exchange column of DEAE‐cellulose yielded RMEP. Structural investigation indicated that the main chain of RMEP was composed of (1 → 3) and (1 → 4)‐linked mannopyranosyl residues, with branches attached to O‐6 of some (1 → 3)‐linked mannopyranosyl residues. The branches were composed of Glcp‐(1 → residues. |
topic |
extracellular polysaccharide response surface methodology Rhodotorula minuta structural study |
url |
https://doi.org/10.1002/fsn3.1792 |
work_keys_str_mv |
AT hamidrezasamadlouie productionmediumoptimizationandstructuralcharacterizationofanextracellularpolysaccharideproducedbyrhodotorulaminutaatcc10658 AT kambizjahanbin productionmediumoptimizationandstructuralcharacterizationofanextracellularpolysaccharideproducedbyrhodotorulaminutaatcc10658 AT parisajalali productionmediumoptimizationandstructuralcharacterizationofanextracellularpolysaccharideproducedbyrhodotorulaminutaatcc10658 |
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1724543997903372288 |