Genetic specification of left–right asymmetry in the diaphragm muscles and their motor innervation
The diaphragm muscle is essential for breathing in mammals. Its asymmetric elevation during contraction correlates with morphological features suggestive of inherent left–right (L/R) asymmetry. Whether this asymmetry is due to L versus R differences in the muscle or in the phrenic nerve activity is...
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2017-06-01
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doaj-c54bf742fcde4492af26ee2d1304e7952021-05-05T13:33:37ZengeLife Sciences Publications LtdeLife2050-084X2017-06-01610.7554/eLife.18481Genetic specification of left–right asymmetry in the diaphragm muscles and their motor innervationCamille Charoy0Sarah Dinvaut1Yohan Chaix2Laurette Morlé3Isabelle Sanyas4Muriel Bozon5Karine Kindbeiter6Bénédicte Durand7Jennifer M Skidmore8Lies De Groef9Motoaki Seki10Lieve Moons11Christiana Ruhrberg12James F Martin13Donna M Martin14Julien Falk15https://orcid.org/0000-0001-8590-5615Valerie Castellani16https://orcid.org/0000-0001-9623-9312University of Lyon, Claude Bernard University Lyon 1, INMG UMR CNRS 5310, INSERM U1217, Lyon, FranceUniversity of Lyon, Claude Bernard University Lyon 1, INMG UMR CNRS 5310, INSERM U1217, Lyon, FranceUniversity of Lyon, Claude Bernard University Lyon 1, INMG UMR CNRS 5310, INSERM U1217, Lyon, FranceUniversity of Lyon, Claude Bernard University Lyon 1, INMG UMR CNRS 5310, INSERM U1217, Lyon, FranceUniversity of Lyon, Claude Bernard University Lyon 1, INMG UMR CNRS 5310, INSERM U1217, Lyon, FranceUniversity of Lyon, Claude Bernard University Lyon 1, INMG UMR CNRS 5310, INSERM U1217, Lyon, FranceUniversity of Lyon, Claude Bernard University Lyon 1, INMG UMR CNRS 5310, INSERM U1217, Lyon, FranceUniversity of Lyon, Claude Bernard University Lyon 1, INMG UMR CNRS 5310, INSERM U1217, Lyon, FranceDepartment of Pediatrics, University of Michigan Medical Center, Ann Arbor, United States; Department of Communicable Diseases, University of Michigan Medical Center, Ann Arbor, United StatesAnimal Physiology and Neurobiology Section, Department of Biology, Laboratory of Neural Circuit Development and Regeneration, Leuven, BelgiumResearch Center for Advanced Science and Technology, University of Tokyo, Tokyo, JapanAnimal Physiology and Neurobiology Section, Department of Biology, Laboratory of Neural Circuit Development and Regeneration, Leuven, BelgiumUCL Institute of Ophthalmology, University College London, London, United KingdomBaylor College of Medicine, Houston, United StatesDepartment of Pediatrics, University of Michigan Medical Center, Ann Arbor, United States; Department of Communicable Diseases, University of Michigan Medical Center, Ann Arbor, United States; Department of Human Genetics, University of Michigan Medical Center, Ann Arbor, United StatesUniversity of Lyon, Claude Bernard University Lyon 1, INMG UMR CNRS 5310, INSERM U1217, Lyon, FranceUniversity of Lyon, Claude Bernard University Lyon 1, INMG UMR CNRS 5310, INSERM U1217, Lyon, FranceThe diaphragm muscle is essential for breathing in mammals. Its asymmetric elevation during contraction correlates with morphological features suggestive of inherent left–right (L/R) asymmetry. Whether this asymmetry is due to L versus R differences in the muscle or in the phrenic nerve activity is unknown. Here, we have combined the analysis of genetically modified mouse models with transcriptomic analysis to show that both the diaphragm muscle and phrenic nerves have asymmetries, which can be established independently of each other during early embryogenesis in pathway instructed by Nodal, a morphogen that also conveys asymmetry in other organs. We further found that phrenic motoneurons receive an early L/R genetic imprint, with L versus R differences both in Slit/Robo signaling and MMP2 activity and in the contribution of both pathways to establish phrenic nerve asymmetry. Our study therefore demonstrates L–R imprinting of spinal motoneurons and describes how L/R modulation of axon guidance signaling helps to match neural circuit formation to organ asymmetry.https://elifesciences.org/articles/18481left/right asymmetrymotoneurondiaphragmNodalSlit/Roboaxon guidance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Camille Charoy Sarah Dinvaut Yohan Chaix Laurette Morlé Isabelle Sanyas Muriel Bozon Karine Kindbeiter Bénédicte Durand Jennifer M Skidmore Lies De Groef Motoaki Seki Lieve Moons Christiana Ruhrberg James F Martin Donna M Martin Julien Falk Valerie Castellani |
spellingShingle |
Camille Charoy Sarah Dinvaut Yohan Chaix Laurette Morlé Isabelle Sanyas Muriel Bozon Karine Kindbeiter Bénédicte Durand Jennifer M Skidmore Lies De Groef Motoaki Seki Lieve Moons Christiana Ruhrberg James F Martin Donna M Martin Julien Falk Valerie Castellani Genetic specification of left–right asymmetry in the diaphragm muscles and their motor innervation eLife left/right asymmetry motoneuron diaphragm Nodal Slit/Robo axon guidance |
author_facet |
Camille Charoy Sarah Dinvaut Yohan Chaix Laurette Morlé Isabelle Sanyas Muriel Bozon Karine Kindbeiter Bénédicte Durand Jennifer M Skidmore Lies De Groef Motoaki Seki Lieve Moons Christiana Ruhrberg James F Martin Donna M Martin Julien Falk Valerie Castellani |
author_sort |
Camille Charoy |
title |
Genetic specification of left–right asymmetry in the diaphragm muscles and their motor innervation |
title_short |
Genetic specification of left–right asymmetry in the diaphragm muscles and their motor innervation |
title_full |
Genetic specification of left–right asymmetry in the diaphragm muscles and their motor innervation |
title_fullStr |
Genetic specification of left–right asymmetry in the diaphragm muscles and their motor innervation |
title_full_unstemmed |
Genetic specification of left–right asymmetry in the diaphragm muscles and their motor innervation |
title_sort |
genetic specification of left–right asymmetry in the diaphragm muscles and their motor innervation |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2017-06-01 |
description |
The diaphragm muscle is essential for breathing in mammals. Its asymmetric elevation during contraction correlates with morphological features suggestive of inherent left–right (L/R) asymmetry. Whether this asymmetry is due to L versus R differences in the muscle or in the phrenic nerve activity is unknown. Here, we have combined the analysis of genetically modified mouse models with transcriptomic analysis to show that both the diaphragm muscle and phrenic nerves have asymmetries, which can be established independently of each other during early embryogenesis in pathway instructed by Nodal, a morphogen that also conveys asymmetry in other organs. We further found that phrenic motoneurons receive an early L/R genetic imprint, with L versus R differences both in Slit/Robo signaling and MMP2 activity and in the contribution of both pathways to establish phrenic nerve asymmetry. Our study therefore demonstrates L–R imprinting of spinal motoneurons and describes how L/R modulation of axon guidance signaling helps to match neural circuit formation to organ asymmetry. |
topic |
left/right asymmetry motoneuron diaphragm Nodal Slit/Robo axon guidance |
url |
https://elifesciences.org/articles/18481 |
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