Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions

Lipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the conversion of synthetically interesting, non-natural subs...

Full description

Bibliographic Details
Main Authors: Sandra Engelskirchen, Stefan Wellert, Olaf Holderer, Henrich Frielinghaus, Michaela Laupheimer, Sven Richter, Bettina Nestl, Bernd Nebel, Bernhard Hauer
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-01-01
Series:Frontiers in Chemistry
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fchem.2020.613388/full
id doaj-c54d50fbd52947128fff02c91c948849
record_format Article
spelling doaj-c54d50fbd52947128fff02c91c9488492021-01-05T10:21:59ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462021-01-01810.3389/fchem.2020.613388613388Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous MicroemulsionsSandra Engelskirchen0Stefan Wellert1Olaf Holderer2Henrich Frielinghaus3Michaela Laupheimer4Sven Richter5Bettina Nestl6Bernd Nebel7Bernhard Hauer8Department of Chemistry, Universität Stuttgart, Stuttgart, GermanyDepartment of Chemistry, Technische Universität Berlin, Berlin, GermanyJülich Centre for Neutron Science (JCNS) at Heinz Maier-Leibnitz Zentrum (MLZ), Forschungszentrum Jülich GmbH, Garching, GermanyJülich Centre for Neutron Science (JCNS) at Heinz Maier-Leibnitz Zentrum (MLZ), Forschungszentrum Jülich GmbH, Garching, GermanyDepartment of Chemistry, Universität Stuttgart, Stuttgart, GermanyDepartment of Chemistry, Universität Stuttgart, Stuttgart, GermanyDepartment of Chemistry, Universität Stuttgart, Stuttgart, GermanyDepartment of Chemistry, Universität Stuttgart, Stuttgart, GermanyDepartment of Chemistry, Universität Stuttgart, Stuttgart, GermanyLipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the conversion of synthetically interesting, non-natural substrates is the substantial insolubility of such substrates in water. The conversion of substrates, natural or non-natural, by lipases generally involves the presence of a water–oil interface. In the present paper, we exploit the fact that the presence of lipases, in particular the lipase from Candida antarctica B (CalB), changes the bending elastic properties of a surfactant monolayer in a bicontinuous microemulsion consisting of D2O/NaCl -n-(d)-octane-pentaethylene glycol monodecyl ether (C10E5) in a similar manner as previously observed for amphiphilic block-copolymers. To determine the bending elastic constant, we have used two approaches, small angle neutron scattering (SANS) and neutron spin echo (NSE) spectroscopy. The time-averaged structure from SANS showed a slight decrease in bending elasticity, while on nanosecond time scales as probed with NSE, a stiffening has been observed, which was attributed to adsorption/desorption mechanisms of CalB at the surfactant monolayer. The results allow to derive further information on the influence of CalB on the composition and bending elasticity of the surfactant monolayer itself as well as the underlying adsorption/desorption mechanism.https://www.frontiersin.org/articles/10.3389/fchem.2020.613388/fullmicroemulsionlipasebending elasticityneutron scatteringneutron spin echo
collection DOAJ
language English
format Article
sources DOAJ
author Sandra Engelskirchen
Stefan Wellert
Olaf Holderer
Henrich Frielinghaus
Michaela Laupheimer
Sven Richter
Bettina Nestl
Bernd Nebel
Bernhard Hauer
spellingShingle Sandra Engelskirchen
Stefan Wellert
Olaf Holderer
Henrich Frielinghaus
Michaela Laupheimer
Sven Richter
Bettina Nestl
Bernd Nebel
Bernhard Hauer
Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions
Frontiers in Chemistry
microemulsion
lipase
bending elasticity
neutron scattering
neutron spin echo
author_facet Sandra Engelskirchen
Stefan Wellert
Olaf Holderer
Henrich Frielinghaus
Michaela Laupheimer
Sven Richter
Bettina Nestl
Bernd Nebel
Bernhard Hauer
author_sort Sandra Engelskirchen
title Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions
title_short Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions
title_full Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions
title_fullStr Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions
title_full_unstemmed Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions
title_sort surfactant monolayer bending elasticity in lipase containing bicontinuous microemulsions
publisher Frontiers Media S.A.
series Frontiers in Chemistry
issn 2296-2646
publishDate 2021-01-01
description Lipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the conversion of synthetically interesting, non-natural substrates is the substantial insolubility of such substrates in water. The conversion of substrates, natural or non-natural, by lipases generally involves the presence of a water–oil interface. In the present paper, we exploit the fact that the presence of lipases, in particular the lipase from Candida antarctica B (CalB), changes the bending elastic properties of a surfactant monolayer in a bicontinuous microemulsion consisting of D2O/NaCl -n-(d)-octane-pentaethylene glycol monodecyl ether (C10E5) in a similar manner as previously observed for amphiphilic block-copolymers. To determine the bending elastic constant, we have used two approaches, small angle neutron scattering (SANS) and neutron spin echo (NSE) spectroscopy. The time-averaged structure from SANS showed a slight decrease in bending elasticity, while on nanosecond time scales as probed with NSE, a stiffening has been observed, which was attributed to adsorption/desorption mechanisms of CalB at the surfactant monolayer. The results allow to derive further information on the influence of CalB on the composition and bending elasticity of the surfactant monolayer itself as well as the underlying adsorption/desorption mechanism.
topic microemulsion
lipase
bending elasticity
neutron scattering
neutron spin echo
url https://www.frontiersin.org/articles/10.3389/fchem.2020.613388/full
work_keys_str_mv AT sandraengelskirchen surfactantmonolayerbendingelasticityinlipasecontainingbicontinuousmicroemulsions
AT stefanwellert surfactantmonolayerbendingelasticityinlipasecontainingbicontinuousmicroemulsions
AT olafholderer surfactantmonolayerbendingelasticityinlipasecontainingbicontinuousmicroemulsions
AT henrichfrielinghaus surfactantmonolayerbendingelasticityinlipasecontainingbicontinuousmicroemulsions
AT michaelalaupheimer surfactantmonolayerbendingelasticityinlipasecontainingbicontinuousmicroemulsions
AT svenrichter surfactantmonolayerbendingelasticityinlipasecontainingbicontinuousmicroemulsions
AT bettinanestl surfactantmonolayerbendingelasticityinlipasecontainingbicontinuousmicroemulsions
AT berndnebel surfactantmonolayerbendingelasticityinlipasecontainingbicontinuousmicroemulsions
AT bernhardhauer surfactantmonolayerbendingelasticityinlipasecontainingbicontinuousmicroemulsions
_version_ 1724348364003213312