Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions
Lipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the conversion of synthetically interesting, non-natural subs...
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doaj-c54d50fbd52947128fff02c91c9488492021-01-05T10:21:59ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462021-01-01810.3389/fchem.2020.613388613388Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous MicroemulsionsSandra Engelskirchen0Stefan Wellert1Olaf Holderer2Henrich Frielinghaus3Michaela Laupheimer4Sven Richter5Bettina Nestl6Bernd Nebel7Bernhard Hauer8Department of Chemistry, Universität Stuttgart, Stuttgart, GermanyDepartment of Chemistry, Technische Universität Berlin, Berlin, GermanyJülich Centre for Neutron Science (JCNS) at Heinz Maier-Leibnitz Zentrum (MLZ), Forschungszentrum Jülich GmbH, Garching, GermanyJülich Centre for Neutron Science (JCNS) at Heinz Maier-Leibnitz Zentrum (MLZ), Forschungszentrum Jülich GmbH, Garching, GermanyDepartment of Chemistry, Universität Stuttgart, Stuttgart, GermanyDepartment of Chemistry, Universität Stuttgart, Stuttgart, GermanyDepartment of Chemistry, Universität Stuttgart, Stuttgart, GermanyDepartment of Chemistry, Universität Stuttgart, Stuttgart, GermanyDepartment of Chemistry, Universität Stuttgart, Stuttgart, GermanyLipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the conversion of synthetically interesting, non-natural substrates is the substantial insolubility of such substrates in water. The conversion of substrates, natural or non-natural, by lipases generally involves the presence of a water–oil interface. In the present paper, we exploit the fact that the presence of lipases, in particular the lipase from Candida antarctica B (CalB), changes the bending elastic properties of a surfactant monolayer in a bicontinuous microemulsion consisting of D2O/NaCl -n-(d)-octane-pentaethylene glycol monodecyl ether (C10E5) in a similar manner as previously observed for amphiphilic block-copolymers. To determine the bending elastic constant, we have used two approaches, small angle neutron scattering (SANS) and neutron spin echo (NSE) spectroscopy. The time-averaged structure from SANS showed a slight decrease in bending elasticity, while on nanosecond time scales as probed with NSE, a stiffening has been observed, which was attributed to adsorption/desorption mechanisms of CalB at the surfactant monolayer. The results allow to derive further information on the influence of CalB on the composition and bending elasticity of the surfactant monolayer itself as well as the underlying adsorption/desorption mechanism.https://www.frontiersin.org/articles/10.3389/fchem.2020.613388/fullmicroemulsionlipasebending elasticityneutron scatteringneutron spin echo |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sandra Engelskirchen Stefan Wellert Olaf Holderer Henrich Frielinghaus Michaela Laupheimer Sven Richter Bettina Nestl Bernd Nebel Bernhard Hauer |
spellingShingle |
Sandra Engelskirchen Stefan Wellert Olaf Holderer Henrich Frielinghaus Michaela Laupheimer Sven Richter Bettina Nestl Bernd Nebel Bernhard Hauer Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions Frontiers in Chemistry microemulsion lipase bending elasticity neutron scattering neutron spin echo |
author_facet |
Sandra Engelskirchen Stefan Wellert Olaf Holderer Henrich Frielinghaus Michaela Laupheimer Sven Richter Bettina Nestl Bernd Nebel Bernhard Hauer |
author_sort |
Sandra Engelskirchen |
title |
Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions |
title_short |
Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions |
title_full |
Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions |
title_fullStr |
Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions |
title_full_unstemmed |
Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions |
title_sort |
surfactant monolayer bending elasticity in lipase containing bicontinuous microemulsions |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Chemistry |
issn |
2296-2646 |
publishDate |
2021-01-01 |
description |
Lipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the conversion of synthetically interesting, non-natural substrates is the substantial insolubility of such substrates in water. The conversion of substrates, natural or non-natural, by lipases generally involves the presence of a water–oil interface. In the present paper, we exploit the fact that the presence of lipases, in particular the lipase from Candida antarctica B (CalB), changes the bending elastic properties of a surfactant monolayer in a bicontinuous microemulsion consisting of D2O/NaCl -n-(d)-octane-pentaethylene glycol monodecyl ether (C10E5) in a similar manner as previously observed for amphiphilic block-copolymers. To determine the bending elastic constant, we have used two approaches, small angle neutron scattering (SANS) and neutron spin echo (NSE) spectroscopy. The time-averaged structure from SANS showed a slight decrease in bending elasticity, while on nanosecond time scales as probed with NSE, a stiffening has been observed, which was attributed to adsorption/desorption mechanisms of CalB at the surfactant monolayer. The results allow to derive further information on the influence of CalB on the composition and bending elasticity of the surfactant monolayer itself as well as the underlying adsorption/desorption mechanism. |
topic |
microemulsion lipase bending elasticity neutron scattering neutron spin echo |
url |
https://www.frontiersin.org/articles/10.3389/fchem.2020.613388/full |
work_keys_str_mv |
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