Mitotic count can predict tamoxifen benefit in postmenopausal breast cancer patients while Ki67 score cannot
Abstract Background Controversy exists for the use of Ki67 protein expression as a predictive marker to select patients who do or do not derive benefit from adjuvant endocrine therapy. Whether other proliferation markers, like Cyclin D1, and mitotic count can also be used to identify those estrogen...
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doaj-c558154a46ac40b6b7bc562fdd869e712020-11-24T21:55:18ZengBMCBMC Cancer1471-24072018-07-0118111110.1186/s12885-018-4516-1Mitotic count can predict tamoxifen benefit in postmenopausal breast cancer patients while Ki67 score cannotKarin Beelen0Mark Opdam1Tesa Severson2Rutger Koornstra3Andrew Vincent4Jelle Wesseling5Joyce Sanders6Jan Vermorken7Paul van Diest8Sabine Linn9Molecular Biology, The Netherlands Cancer InstituteMolecular Biology, The Netherlands Cancer InstituteMolecular Biology, The Netherlands Cancer InstituteMolecular Biology, The Netherlands Cancer InstituteDepartments of Biometrics, The Netherlands Cancer InstitutePathology, The Netherlands Cancer InstitutePathology, The Netherlands Cancer InstituteDepartment of Medical Oncology, University Hospital AntwerpenDepartment of Pathology, University Medical Center UtrechtMolecular Biology, The Netherlands Cancer InstituteAbstract Background Controversy exists for the use of Ki67 protein expression as a predictive marker to select patients who do or do not derive benefit from adjuvant endocrine therapy. Whether other proliferation markers, like Cyclin D1, and mitotic count can also be used to identify those estrogen receptor α (ERα) positive breast cancer patients that derive benefit from tamoxifen is not well established. We tested the predictive value of these markers for tamoxifen benefit in ERα positive postmenopausal breast cancer patients. Methods We collected primary tumor blocks from 563 ERα positive patients who had been randomized between tamoxifen (1 to 3 years) vs. no adjuvant therapy (IKA trial) with a median follow-up of 7.8 years. Mitotic count, Ki67 and Cyclin D1 protein expression were centrally assessed by immunohistochemistry on tissue microarrays. In addition, we tested the predictive value of CCND1 gene copy number variation using MLPA technology. Multivariate Cox proportional hazard models including interaction between marker and treatment were used to test the predictive value of these markers. Results Patients with high Ki67 (≥5%) as well as low (< 5%) expressing tumors equally benefitted from adjuvant tamoxifen (adjusted hazard ratio (HR) 0.5 for both groups)(p for interaction 0.97). We did not observe a significant interaction between either Cyclin D1 or Ki67 and tamoxifen, indicating that the relative benefit from tamoxifen was not dependent on the level of these markers. Patients with tumors with low mitotic count derived substantial benefit from tamoxifen (adjusted HR 0.24, p < 0.0001), while patients with tumors with high mitotic count derived no significant benefit (adjusted HR 0.64, p = 0.14) (p for interaction 0.03). Conclusion Postmenopausal breast cancer patients with high Ki67 counts do significantly benefit from adjuvant tamoxifen, while those with high mitotic count do not. Mitotic count is a better selection marker for reduced tamoxifen benefit than Ki67.http://link.springer.com/article/10.1186/s12885-018-4516-1Breast cancerTamoxifenCell proliferationKi67Mitotic count |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Karin Beelen Mark Opdam Tesa Severson Rutger Koornstra Andrew Vincent Jelle Wesseling Joyce Sanders Jan Vermorken Paul van Diest Sabine Linn |
spellingShingle |
Karin Beelen Mark Opdam Tesa Severson Rutger Koornstra Andrew Vincent Jelle Wesseling Joyce Sanders Jan Vermorken Paul van Diest Sabine Linn Mitotic count can predict tamoxifen benefit in postmenopausal breast cancer patients while Ki67 score cannot BMC Cancer Breast cancer Tamoxifen Cell proliferation Ki67 Mitotic count |
author_facet |
Karin Beelen Mark Opdam Tesa Severson Rutger Koornstra Andrew Vincent Jelle Wesseling Joyce Sanders Jan Vermorken Paul van Diest Sabine Linn |
author_sort |
Karin Beelen |
title |
Mitotic count can predict tamoxifen benefit in postmenopausal breast cancer patients while Ki67 score cannot |
title_short |
Mitotic count can predict tamoxifen benefit in postmenopausal breast cancer patients while Ki67 score cannot |
title_full |
Mitotic count can predict tamoxifen benefit in postmenopausal breast cancer patients while Ki67 score cannot |
title_fullStr |
Mitotic count can predict tamoxifen benefit in postmenopausal breast cancer patients while Ki67 score cannot |
title_full_unstemmed |
Mitotic count can predict tamoxifen benefit in postmenopausal breast cancer patients while Ki67 score cannot |
title_sort |
mitotic count can predict tamoxifen benefit in postmenopausal breast cancer patients while ki67 score cannot |
publisher |
BMC |
series |
BMC Cancer |
issn |
1471-2407 |
publishDate |
2018-07-01 |
description |
Abstract Background Controversy exists for the use of Ki67 protein expression as a predictive marker to select patients who do or do not derive benefit from adjuvant endocrine therapy. Whether other proliferation markers, like Cyclin D1, and mitotic count can also be used to identify those estrogen receptor α (ERα) positive breast cancer patients that derive benefit from tamoxifen is not well established. We tested the predictive value of these markers for tamoxifen benefit in ERα positive postmenopausal breast cancer patients. Methods We collected primary tumor blocks from 563 ERα positive patients who had been randomized between tamoxifen (1 to 3 years) vs. no adjuvant therapy (IKA trial) with a median follow-up of 7.8 years. Mitotic count, Ki67 and Cyclin D1 protein expression were centrally assessed by immunohistochemistry on tissue microarrays. In addition, we tested the predictive value of CCND1 gene copy number variation using MLPA technology. Multivariate Cox proportional hazard models including interaction between marker and treatment were used to test the predictive value of these markers. Results Patients with high Ki67 (≥5%) as well as low (< 5%) expressing tumors equally benefitted from adjuvant tamoxifen (adjusted hazard ratio (HR) 0.5 for both groups)(p for interaction 0.97). We did not observe a significant interaction between either Cyclin D1 or Ki67 and tamoxifen, indicating that the relative benefit from tamoxifen was not dependent on the level of these markers. Patients with tumors with low mitotic count derived substantial benefit from tamoxifen (adjusted HR 0.24, p < 0.0001), while patients with tumors with high mitotic count derived no significant benefit (adjusted HR 0.64, p = 0.14) (p for interaction 0.03). Conclusion Postmenopausal breast cancer patients with high Ki67 counts do significantly benefit from adjuvant tamoxifen, while those with high mitotic count do not. Mitotic count is a better selection marker for reduced tamoxifen benefit than Ki67. |
topic |
Breast cancer Tamoxifen Cell proliferation Ki67 Mitotic count |
url |
http://link.springer.com/article/10.1186/s12885-018-4516-1 |
work_keys_str_mv |
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