CD1c-related DCs that express CD207/langerin, but are distinguishable from Langerhans cells, are consistently present in human tonsils
Several subsets of dendritic cells (DCs) are present in the oropharyngeal tonsillar tissues and are thought to behave as major actors in development and regulation of immunity by acting as a first line of recognition for airborne and alimentary antigens. We previously discovered in human adult tonsi...
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doaj-c560c185dd8e4b52970abf710d664a462020-11-24T21:28:25ZengFrontiers Media S.A.Frontiers in Immunology1664-32242016-05-01710.3389/fimmu.2016.00197197497CD1c-related DCs that express CD207/langerin, but are distinguishable from Langerhans cells, are consistently present in human tonsilsAnne eDe Monte0Anne eDe Monte1Anne eDe Monte2Charles-Vivien eOLIVIERI3Charles-Vivien eOLIVIERI4Sebastien eVITALE5Sonanda eBAILLEUX6Laurent eCASTILLO7Valerie eGIORDANENGO8Janet L MARYANSKI9Janet L MARYANSKI10Elodie eSegura11Alain eDOGLIO12Alain eDOGLIO13Université Nice-Sophia-AntipolisCentre Hospitalier Universitaire de NiceCentre Hospitalier Universitaire de NiceUniversité Nice-Sophia-AntipolisCentre Hospitalier Universitaire de NiceCentre Hospitalier Universitaire de NiceHôpitaux pédiatriques de Nice CHU-LenvalInstitut Universitaire de la Face et du Cou (IUFC)Centre Hospitalier Universitaire de NiceUniversité Nice-Sophia-AntipolisCentre Hospitalier Universitaire de NiceInstitut CurieUniversité Nice-Sophia-AntipolisCentre Hospitalier Universitaire de NiceSeveral subsets of dendritic cells (DCs) are present in the oropharyngeal tonsillar tissues and are thought to behave as major actors in development and regulation of immunity by acting as a first line of recognition for airborne and alimentary antigens. We previously discovered in human adult tonsils infected with Epstein-Barr Virus (EBV) a subset of DCs that expressed langerin/CD207, a lectin usually recognized as a hallmark of epidermal Langerhans cells (LCs). In the present study, we analyzed the content of several child and adult tonsils in order to characterize in more detail the phenotype of these tonsillar CD207-expressing DCs (tCD207 DCs) and to compare it with that of other human DC subsets. We showed that all the human tonsils studied (n=12) contained significant proportions of tCD207 DCs among tonsillar cells expressing HLA-DR. Moreover, the presence of tCD207 DCs in tonsils from young children free of EBV infection indicated that these cells could be established early in the tonsil independently of EBV infection. We also showed that tCD207 DCs, that were found mainly located within the tonsillar lymphoid stroma, were distinguishable from LCs by the level of expression of CD1a and EpCAM, and also from human inflammatory DCs (infDCs) by the lack of CD1a, CD206 and CD14 expression. Detailed analysis of cell surface DC markers showed that tCD207 DCs were unrelated to CD141+ DCs or macrophages, but defined a subtype of tonsillar DCs closely related to myeloid resident CD1c DCs. Since it was established that blood CD1c myeloid DCs exhibit plasticity and are capable of expressing CD207 notably in the presence of inflammatory cytokines, it is tempting to speculate that CD207+ CD1c+ DCs may play a specific immune role.http://journal.frontiersin.org/Journal/10.3389/fimmu.2016.00197/fullDendritic CellsLangerhans CellstonsilInflammatory dendritic cellslangerin/CD207CD1c myeloid dendritic cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anne eDe Monte Anne eDe Monte Anne eDe Monte Charles-Vivien eOLIVIERI Charles-Vivien eOLIVIERI Sebastien eVITALE Sonanda eBAILLEUX Laurent eCASTILLO Valerie eGIORDANENGO Janet L MARYANSKI Janet L MARYANSKI Elodie eSegura Alain eDOGLIO Alain eDOGLIO |
spellingShingle |
Anne eDe Monte Anne eDe Monte Anne eDe Monte Charles-Vivien eOLIVIERI Charles-Vivien eOLIVIERI Sebastien eVITALE Sonanda eBAILLEUX Laurent eCASTILLO Valerie eGIORDANENGO Janet L MARYANSKI Janet L MARYANSKI Elodie eSegura Alain eDOGLIO Alain eDOGLIO CD1c-related DCs that express CD207/langerin, but are distinguishable from Langerhans cells, are consistently present in human tonsils Frontiers in Immunology Dendritic Cells Langerhans Cells tonsil Inflammatory dendritic cells langerin/CD207 CD1c myeloid dendritic cells |
author_facet |
Anne eDe Monte Anne eDe Monte Anne eDe Monte Charles-Vivien eOLIVIERI Charles-Vivien eOLIVIERI Sebastien eVITALE Sonanda eBAILLEUX Laurent eCASTILLO Valerie eGIORDANENGO Janet L MARYANSKI Janet L MARYANSKI Elodie eSegura Alain eDOGLIO Alain eDOGLIO |
author_sort |
Anne eDe Monte |
title |
CD1c-related DCs that express CD207/langerin, but are distinguishable from Langerhans cells, are consistently present in human tonsils |
title_short |
CD1c-related DCs that express CD207/langerin, but are distinguishable from Langerhans cells, are consistently present in human tonsils |
title_full |
CD1c-related DCs that express CD207/langerin, but are distinguishable from Langerhans cells, are consistently present in human tonsils |
title_fullStr |
CD1c-related DCs that express CD207/langerin, but are distinguishable from Langerhans cells, are consistently present in human tonsils |
title_full_unstemmed |
CD1c-related DCs that express CD207/langerin, but are distinguishable from Langerhans cells, are consistently present in human tonsils |
title_sort |
cd1c-related dcs that express cd207/langerin, but are distinguishable from langerhans cells, are consistently present in human tonsils |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2016-05-01 |
description |
Several subsets of dendritic cells (DCs) are present in the oropharyngeal tonsillar tissues and are thought to behave as major actors in development and regulation of immunity by acting as a first line of recognition for airborne and alimentary antigens. We previously discovered in human adult tonsils infected with Epstein-Barr Virus (EBV) a subset of DCs that expressed langerin/CD207, a lectin usually recognized as a hallmark of epidermal Langerhans cells (LCs). In the present study, we analyzed the content of several child and adult tonsils in order to characterize in more detail the phenotype of these tonsillar CD207-expressing DCs (tCD207 DCs) and to compare it with that of other human DC subsets. We showed that all the human tonsils studied (n=12) contained significant proportions of tCD207 DCs among tonsillar cells expressing HLA-DR. Moreover, the presence of tCD207 DCs in tonsils from young children free of EBV infection indicated that these cells could be established early in the tonsil independently of EBV infection. We also showed that tCD207 DCs, that were found mainly located within the tonsillar lymphoid stroma, were distinguishable from LCs by the level of expression of CD1a and EpCAM, and also from human inflammatory DCs (infDCs) by the lack of CD1a, CD206 and CD14 expression. Detailed analysis of cell surface DC markers showed that tCD207 DCs were unrelated to CD141+ DCs or macrophages, but defined a subtype of tonsillar DCs closely related to myeloid resident CD1c DCs. Since it was established that blood CD1c myeloid DCs exhibit plasticity and are capable of expressing CD207 notably in the presence of inflammatory cytokines, it is tempting to speculate that CD207+ CD1c+ DCs may play a specific immune role. |
topic |
Dendritic Cells Langerhans Cells tonsil Inflammatory dendritic cells langerin/CD207 CD1c myeloid dendritic cells |
url |
http://journal.frontiersin.org/Journal/10.3389/fimmu.2016.00197/full |
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