Determination of phenolic compounds and antioxidant activity of a Mediterranean plant: The case of Satureja montana subsp. kitaibelii
Satureja montana subsp. kitaibelii extract was analyzed by HPLC–DAD–ESI–TOF–MS (high performance liquid chromatography–diode array detector–electrospray ionization–time of flight–mass spectroscopy). Forty five compounds were identified, 42 of which were identified for the first time in this plant. Q...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2015-10-01
|
Series: | Journal of Functional Foods |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464614003296 |
id |
doaj-c56f8f1048a644678bc3e73a0c6dd5df |
---|---|
record_format |
Article |
spelling |
doaj-c56f8f1048a644678bc3e73a0c6dd5df2021-04-29T04:43:15ZengElsevierJournal of Functional Foods1756-46462015-10-011811671178Determination of phenolic compounds and antioxidant activity of a Mediterranean plant: The case of Satureja montana subsp. kitaibeliiAna López-Cobo0Ana María Gómez-Caravaca1Jaroslava Švarc-Gajić2Antonio Segura-Carretero3Alberto Fernández-Gutiérrez4Research and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. del Conocimiento s/n, Edificio Bioregión, Granada 18007, SpainResearch and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. del Conocimiento s/n, Edificio Bioregión, Granada 18007, Spain; Department of Analytical Chemistry, University of Granada, c/Fuentenueva s/n, Granada 18071, SpainDepartment for Applied and Engineering Chemistry, Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, Novi Sad 2100, SerbiaResearch and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. del Conocimiento s/n, Edificio Bioregión, Granada 18007, Spain; Department of Analytical Chemistry, University of Granada, c/Fuentenueva s/n, Granada 18071, Spain; Corresponding author. Department of Analytical Chemistry, University of Granada, c/Fuentenueva s/n, 18071 Granada, Spain. Tel.: +34 958 243296; fax: +34 958 249510.Research and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. del Conocimiento s/n, Edificio Bioregión, Granada 18007, Spain; Department of Analytical Chemistry, University of Granada, c/Fuentenueva s/n, Granada 18071, SpainSatureja montana subsp. kitaibelii extract was analyzed by HPLC–DAD–ESI–TOF–MS (high performance liquid chromatography–diode array detector–electrospray ionization–time of flight–mass spectroscopy). Forty five compounds were identified, 42 of which were identified for the first time in this plant. Quantification of the identified phenolic compounds by UV–vis (ultraviolet–visible) detection was done and chlorogenic acid was the most concentrated compound. Total phenolic content (TPC) by Folin–Ciocalteu method and antioxidant activity by DPPH and ABTS methods were also measured. TPC quantified by HPLC–DAD were 1958.44 mg/100 g dry sample. This is in agreement with TPC measured by Folin–Ciocalteu method. Antioxidant activity determined by the DPPH method expressed as EC50 was 116.36 ± 12.83 µg/mL and by the ABTS method was 106.59 ± 1.41 µmol trolox equivalents/g dry sample. These results reveal that Satureja montana subsp. kitaibelii could be a good functional food ingredient because its high content of natural antioxidants as demonstrated by quantification with HPLC–DAD.http://www.sciencedirect.com/science/article/pii/S1756464614003296Satureja montana subsp. kitaibeliiHPLC–DAD–ESI–TOF–MSAntioxidant activityQuantificationTotal phenolic compounds |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana López-Cobo Ana María Gómez-Caravaca Jaroslava Švarc-Gajić Antonio Segura-Carretero Alberto Fernández-Gutiérrez |
spellingShingle |
Ana López-Cobo Ana María Gómez-Caravaca Jaroslava Švarc-Gajić Antonio Segura-Carretero Alberto Fernández-Gutiérrez Determination of phenolic compounds and antioxidant activity of a Mediterranean plant: The case of Satureja montana subsp. kitaibelii Journal of Functional Foods Satureja montana subsp. kitaibelii HPLC–DAD–ESI–TOF–MS Antioxidant activity Quantification Total phenolic compounds |
author_facet |
Ana López-Cobo Ana María Gómez-Caravaca Jaroslava Švarc-Gajić Antonio Segura-Carretero Alberto Fernández-Gutiérrez |
author_sort |
Ana López-Cobo |
title |
Determination of phenolic compounds and antioxidant activity of a Mediterranean plant: The case of Satureja montana subsp. kitaibelii |
title_short |
Determination of phenolic compounds and antioxidant activity of a Mediterranean plant: The case of Satureja montana subsp. kitaibelii |
title_full |
Determination of phenolic compounds and antioxidant activity of a Mediterranean plant: The case of Satureja montana subsp. kitaibelii |
title_fullStr |
Determination of phenolic compounds and antioxidant activity of a Mediterranean plant: The case of Satureja montana subsp. kitaibelii |
title_full_unstemmed |
Determination of phenolic compounds and antioxidant activity of a Mediterranean plant: The case of Satureja montana subsp. kitaibelii |
title_sort |
determination of phenolic compounds and antioxidant activity of a mediterranean plant: the case of satureja montana subsp. kitaibelii |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2015-10-01 |
description |
Satureja montana subsp. kitaibelii extract was analyzed by HPLC–DAD–ESI–TOF–MS (high performance liquid chromatography–diode array detector–electrospray ionization–time of flight–mass spectroscopy). Forty five compounds were identified, 42 of which were identified for the first time in this plant. Quantification of the identified phenolic compounds by UV–vis (ultraviolet–visible) detection was done and chlorogenic acid was the most concentrated compound. Total phenolic content (TPC) by Folin–Ciocalteu method and antioxidant activity by DPPH and ABTS methods were also measured. TPC quantified by HPLC–DAD were 1958.44 mg/100 g dry sample. This is in agreement with TPC measured by Folin–Ciocalteu method. Antioxidant activity determined by the DPPH method expressed as EC50 was 116.36 ± 12.83 µg/mL and by the ABTS method was 106.59 ± 1.41 µmol trolox equivalents/g dry sample. These results reveal that Satureja montana subsp. kitaibelii could be a good functional food ingredient because its high content of natural antioxidants as demonstrated by quantification with HPLC–DAD. |
topic |
Satureja montana subsp. kitaibelii HPLC–DAD–ESI–TOF–MS Antioxidant activity Quantification Total phenolic compounds |
url |
http://www.sciencedirect.com/science/article/pii/S1756464614003296 |
work_keys_str_mv |
AT analopezcobo determinationofphenoliccompoundsandantioxidantactivityofamediterraneanplantthecaseofsaturejamontanasubspkitaibelii AT anamariagomezcaravaca determinationofphenoliccompoundsandantioxidantactivityofamediterraneanplantthecaseofsaturejamontanasubspkitaibelii AT jaroslavasvarcgajic determinationofphenoliccompoundsandantioxidantactivityofamediterraneanplantthecaseofsaturejamontanasubspkitaibelii AT antonioseguracarretero determinationofphenoliccompoundsandantioxidantactivityofamediterraneanplantthecaseofsaturejamontanasubspkitaibelii AT albertofernandezgutierrez determinationofphenoliccompoundsandantioxidantactivityofamediterraneanplantthecaseofsaturejamontanasubspkitaibelii |
_version_ |
1721501873691688960 |