CO₂ Laser Photoacoustic Spectrometer for Measuring Acetone in the Breath of Lung Cancer Patients

• Main Authors: Mitrayana, Donni Kis Apriyanto, Mirza Satriawan
• Format: Article
• Language: English
• Published: MDPI AG 2020-05-01
• Series: Biosensors
• Subjects: lung cancer; acetone; volatile organic compounds; CO2 laser photoacoustic
• Online Access: https://www.mdpi.com/2079-6374/10/6/55

A CO₂ laser has the advantages of being high in power and having many laser lines in the 9–11 µm infrared region. Thus, a CO₂ laser photoacoustic spectrometer (PAS) can have a multi-component measurement capability for many gas compounds that have non-zero absorption coefficients at the laser lines, and therefore can be applied for measuring several volatile organic compounds (VOCs) in the human breath. We have developed a CO₂ laser PAS system for detecting acetone in the human breath. Although acetone has small absorption coefficients at the CO₂ laser lines, our PAS system was able to obtain strong photoacoustic (PA) signals at several CO₂ laser lines, with the strongest one being at the 10P20 line. Since at the 10P20 line, ethylene and ammonia also have significant absorption coefficients, these two gases have to be included in a multi-component measurement with acetone. We obtained the lowest detection limit of our system for the ethylene, acetone, and ammonia are 6 ppbv, 11 ppbv, and 31 ppbv, respectively. We applied our PAS system to measure these three VOCs in the breath of three groups of subjects, i.e., patients with lung cancer disease, patients with other lung diseases, and healthy volunteers.