Solution Structure of the Circular γ-Domain Analog from the Wheat Metallothionein Ec-1

The first cyclic analog of a metallothionein (MT) was prepared and analyzed by UV and (magnetic) circular dichroism spectroscopy, ESI-MS as well as NMR spectroscopy. Results reveal that the evaluated cyclic g-Ec-1 domain of the wheat MT Ec-1 retains its ability to coordinate two Zn(II) or Cd(II) ion...

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Main Authors:	Katsiaryna Tarasava, Silke Johannsen, Eva Freisinger
Format:	Article
Language:	English
Published:	MDPI AG 2013-11-01
Series:	Molecules
Subjects:	plant metallothioneins metal-thiolate cluster backbone cyclization flexibility reduction NMR spectroscopy
Online Access:	http://www.mdpi.com/1420-3049/18/11/14414

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spelling	doaj-c593beb69e884e84a0cb68a1a27005ef2020-11-24T22:57:45ZengMDPI AGMolecules1420-30492013-11-011811144141442910.3390/molecules181114414molecules181114414Solution Structure of the Circular γ-Domain Analog from the Wheat Metallothionein Ec-1Katsiaryna Tarasava0Silke Johannsen1Eva Freisinger2Institute of Inorganic Chemistry, University of Zurich, Winterthurerstrasse 190, Zurich CH-8057, SwitzerlandInstitute of Inorganic Chemistry, University of Zurich, Winterthurerstrasse 190, Zurich CH-8057, SwitzerlandInstitute of Inorganic Chemistry, University of Zurich, Winterthurerstrasse 190, Zurich CH-8057, SwitzerlandThe first cyclic analog of a metallothionein (MT) was prepared and analyzed by UV and (magnetic) circular dichroism spectroscopy, ESI-MS as well as NMR spectroscopy. Results reveal that the evaluated cyclic g-Ec-1 domain of the wheat MT Ec-1 retains its ability to coordinate two Zn(II) or Cd(II) ions and adopts a three-dimensional structure that is highly similar to the one of the linear wild-type form. However, the reduced flexibility of the protein backbone facilitates structure solution significantly and results in a certain stabilization of metal binding to the protein.http://www.mdpi.com/1420-3049/18/11/14414plant metallothioneinsmetal-thiolate clusterbackbone cyclizationflexibility reductionNMR spectroscopy
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Katsiaryna Tarasava Silke Johannsen Eva Freisinger
spellingShingle	Katsiaryna Tarasava Silke Johannsen Eva Freisinger Solution Structure of the Circular γ-Domain Analog from the Wheat Metallothionein Ec-1 Molecules plant metallothioneins metal-thiolate cluster backbone cyclization flexibility reduction NMR spectroscopy
author_facet	Katsiaryna Tarasava Silke Johannsen Eva Freisinger
author_sort	Katsiaryna Tarasava
title	Solution Structure of the Circular γ-Domain Analog from the Wheat Metallothionein Ec-1
title_short	Solution Structure of the Circular γ-Domain Analog from the Wheat Metallothionein Ec-1
title_full	Solution Structure of the Circular γ-Domain Analog from the Wheat Metallothionein Ec-1
title_fullStr	Solution Structure of the Circular γ-Domain Analog from the Wheat Metallothionein Ec-1
title_full_unstemmed	Solution Structure of the Circular γ-Domain Analog from the Wheat Metallothionein Ec-1
title_sort	solution structure of the circular γ-domain analog from the wheat metallothionein ec-1
publisher	MDPI AG
series	Molecules
issn	1420-3049
publishDate	2013-11-01
description	The first cyclic analog of a metallothionein (MT) was prepared and analyzed by UV and (magnetic) circular dichroism spectroscopy, ESI-MS as well as NMR spectroscopy. Results reveal that the evaluated cyclic g-Ec-1 domain of the wheat MT Ec-1 retains its ability to coordinate two Zn(II) or Cd(II) ions and adopts a three-dimensional structure that is highly similar to the one of the linear wild-type form. However, the reduced flexibility of the protein backbone facilitates structure solution significantly and results in a certain stabilization of metal binding to the protein.
topic	plant metallothioneins metal-thiolate cluster backbone cyclization flexibility reduction NMR spectroscopy
url	http://www.mdpi.com/1420-3049/18/11/14414
work_keys_str_mv	AT katsiarynatarasava solutionstructureofthecirculargdomainanalogfromthewheatmetallothioneinec1 AT silkejohannsen solutionstructureofthecirculargdomainanalogfromthewheatmetallothioneinec1 AT evafreisinger solutionstructureofthecirculargdomainanalogfromthewheatmetallothioneinec1
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Solution Structure of the Circular γ-Domain Analog from the Wheat Metallothionein Ec-1

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