In vitro Antitumour Activity of Tomato-Extracted Carotenoids on Human Colorectal Carcinoma

• Main Authors: Diana CENARIU, Eva FISCHER-FODOR, Piroska VIRAG, Corina TATOMIR, Mihai CENARIU, Emoke PALL, Adela PINTEA, Andrei MOCAN, Ioan SIMON, Gianina CRISAN
• Format: Article
• Language: English
• Published: AcademicPres 2015-12-01
• Series: Notulae Botanicae Horti Agrobotanici Cluj-Napoca
• Online Access: http://www.notulaebotanicae.ro/index.php/nbha/article/view/9982
• ISSN: 0255-965X; 1842-4309

<p>The aim of this research was to establish whether <em>all-trans</em> lycopene extracted from fresh and frozen tomatoes is able to inhibit the <em>in vitro</em> proliferation of colon cancer cells, to trigger apoptosis by reactive oxygen species modulation and to reveal its influence on NF-kβ signalling, through the p65 transcription factor and expression of two TNF receptors: GITR and CD27. The carotenoid extracts containing <em>all-trans</em> lycopene were obtained from fresh (E1) and frozen/thawed (E2) tomatoes (<em>Lycopersicon esculentum Mill.</em>), hybrid ‘Menhir’ F1. DLD-1 and HT-29 human colon adenocarcinoma cell lines were co-cultivated with the two extracts and cytotoxicity, apoptosis, antioxidant activity, reactive oxygen species as well as modulation of NF-kβ signalling pathway were assessed. Tomato extracts E1 and E2 were able to inhibit colon cancer cell growth <em>in vitro</em>. E2 contained a higher proportion of <em>all-trans</em> lycopene and displayed superior cytotoxicity and a better apoptosis inducing capacity. The two extracts proved antioxidant activity against DPPH radicals and were able to scavenge the reactive oxygen species in the treated tumour cells. This study also showed that lycopene acts mainly through p65 protein and moderately by TNF receptors GITR and CD27 to deactivate the NF-kβ signalling pathway involved in cancer cell proliferation.</p>