The Effects of Sulglycotide on the Adhesion and the Inflammation of <i>Helicobacter Pylori</i>
<i>Helicobacter pylori</i> (<i>H. pylori</i>) is a primary etiologic factor in gastric diseases. Sulglycotide is a glycopeptide derived from pig duodenal mucin. Esterification of its carbohydrate chains with sulfate groups creates a potent gastroprotective agent used to treat...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-04-01
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Series: | International Journal of Environmental Research and Public Health |
Subjects: | |
Online Access: | https://www.mdpi.com/1660-4601/17/8/2918 |
Summary: | <i>Helicobacter pylori</i> (<i>H. pylori</i>) is a primary etiologic factor in gastric diseases. Sulglycotide is a glycopeptide derived from pig duodenal mucin. Esterification of its carbohydrate chains with sulfate groups creates a potent gastroprotective agent used to treat various gastric diseases. We investigated the inhibitory effects of sulglycotide on adhesion and inflammation after <i>H. pylori</i> infection in human gastric adenocarcinoma cells (AGS cells). <i>H. pylori</i> reference strain 60190 (ATCC 49503) was cultured on Brucella agar supplemented with 10% bovine serum. Sulgylcotide-mediated growth inhibition of <i>H. pylori</i> was evaluated using the broth dilution method. Inhibition of <i>H. pylori</i> adhesion to AGS cells by sulglycotide was assessed using a urease assay. Effects of sulglycotide on the translocation of virulence factors was measured using western blot to detect cytotoxin-associated protein A (CagA) and vacuolating cytotoxin A (VacA) proteins. Inhibition of IL-8 secretion was measured using enzyme-linked immunosorbent assay (ELISA) to determine the effects of sulglycotide on inflammation. Sulglycotide did not inhibit the growth of <i>H. pylori</i>, however, after six and 12 hours of infection on AGS cells, <i>H. pylori</i> adhesion was significantly inhibited by approximately 60% by various concentrations of sulglycotide. Sulglycotide decreased <i>H. pylori </i>virulence factor (CagA and VacA) translocation to AGS cells and inhibited IL-8 secretion. Sulglycotide inhibited <i>H. pylori</i> adhesion and inflammation after infection of AGS cells in vitro. These results support the use of sulglycotide to treat <i>H. pylori</i> infections. |
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ISSN: | 1661-7827 1660-4601 |