Characterization and Expression Analysis of Starch Branching Enzymes in Sweet Potato
Spatial and temporal expression patterns of Sbe1 and Sbe2 that encode starch branching enzyme (SBE) I and II, respectively, in sweet potato (Ipomoea batatas L.) were analyzed. Expression of both genes in Escherichia coli indicate that both genes encoded active SBE. Analysis with real-time quantitati...
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doaj-c5b72134f42b47d1b5a84a59f91d32db2021-06-07T06:48:43ZengElsevierJournal of Integrative Agriculture2095-31192013-09-0112915301539Characterization and Expression Analysis of Starch Branching Enzymes in Sweet PotatoHua QIN0Shuang ZHOU1Yi-zheng ZHANG2QIN Hua, Correspondence ZHANG Yi-zheng, Tel/Fax: +86-28-85412738; College of Life Sciences, Sichuan University, Chengdu 610064, P.R. ChinaCollege of Life Sciences, Sichuan University, Chengdu 610064, P.R. ChinaQIN Hua, Correspondence ZHANG Yi-zheng, Tel/Fax: +86-28-85412738; College of Life Sciences, Sichuan University, Chengdu 610064, P.R. ChinaSpatial and temporal expression patterns of Sbe1 and Sbe2 that encode starch branching enzyme (SBE) I and II, respectively, in sweet potato (Ipomoea batatas L.) were analyzed. Expression of both genes in Escherichia coli indicate that both genes encoded active SBE. Analysis with real-time quantitative polymerase chain reaction technique indicates that IbSbe1 mRNA was expressed at very low levels in leaves but was the predominant isoform in tuberous root while the reverse case was found for IbSbe2. The expression pattern of IbSbe1, closely resembles that of AGPase S, a gene coding for one of the subunits of ADP-glucose pyrophosphorylase, which is the key regulatory enzyme in the starch biosynthetic pathway. Western analysis detected at least two isoforms of SBE I in tuberous roots, those two isoforms showed adverse expression patterns with the development of the tuberous roots. Expression of the two IbSbe genes exhibited a diurnal rhythm during a 12-h cycle when fed a continuous solution of sucrose. Abscisic acid (ABA) was aother potent inducer of IbSbe expression, but bypassed the semidian oscillator.http://www.sciencedirect.com/science/article/pii/S209531191360369Xsweet potatostarch branching enzymecDNA cloningcDNA expressionexpression patternssucrose induction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hua QIN Shuang ZHOU Yi-zheng ZHANG |
spellingShingle |
Hua QIN Shuang ZHOU Yi-zheng ZHANG Characterization and Expression Analysis of Starch Branching Enzymes in Sweet Potato Journal of Integrative Agriculture sweet potato starch branching enzyme cDNA cloning cDNA expression expression patterns sucrose induction |
author_facet |
Hua QIN Shuang ZHOU Yi-zheng ZHANG |
author_sort |
Hua QIN |
title |
Characterization and Expression Analysis of Starch Branching Enzymes in Sweet Potato |
title_short |
Characterization and Expression Analysis of Starch Branching Enzymes in Sweet Potato |
title_full |
Characterization and Expression Analysis of Starch Branching Enzymes in Sweet Potato |
title_fullStr |
Characterization and Expression Analysis of Starch Branching Enzymes in Sweet Potato |
title_full_unstemmed |
Characterization and Expression Analysis of Starch Branching Enzymes in Sweet Potato |
title_sort |
characterization and expression analysis of starch branching enzymes in sweet potato |
publisher |
Elsevier |
series |
Journal of Integrative Agriculture |
issn |
2095-3119 |
publishDate |
2013-09-01 |
description |
Spatial and temporal expression patterns of Sbe1 and Sbe2 that encode starch branching enzyme (SBE) I and II, respectively, in sweet potato (Ipomoea batatas L.) were analyzed. Expression of both genes in Escherichia coli indicate that both genes encoded active SBE. Analysis with real-time quantitative polymerase chain reaction technique indicates that IbSbe1 mRNA was expressed at very low levels in leaves but was the predominant isoform in tuberous root while the reverse case was found for IbSbe2. The expression pattern of IbSbe1, closely resembles that of AGPase S, a gene coding for one of the subunits of ADP-glucose pyrophosphorylase, which is the key regulatory enzyme in the starch biosynthetic pathway. Western analysis detected at least two isoforms of SBE I in tuberous roots, those two isoforms showed adverse expression patterns with the development of the tuberous roots. Expression of the two IbSbe genes exhibited a diurnal rhythm during a 12-h cycle when fed a continuous solution of sucrose. Abscisic acid (ABA) was aother potent inducer of IbSbe expression, but bypassed the semidian oscillator. |
topic |
sweet potato starch branching enzyme cDNA cloning cDNA expression expression patterns sucrose induction |
url |
http://www.sciencedirect.com/science/article/pii/S209531191360369X |
work_keys_str_mv |
AT huaqin characterizationandexpressionanalysisofstarchbranchingenzymesinsweetpotato AT shuangzhou characterizationandexpressionanalysisofstarchbranchingenzymesinsweetpotato AT yizhengzhang characterizationandexpressionanalysisofstarchbranchingenzymesinsweetpotato |
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1714536669650092032 |