IDENTIFICATION OF BETA-LACTOGLOBULIN AND KAPPACASEIN GENOTYPES IN CATTLE
Beta-lactoglobulin (b-Lg) and kappa-casein (k-Cn) are two of the most important proteins in the mammals’ milk synthesized by the epithelial cells of the mammary glands. They play a crucial role in the milk quality and coagulation process (production of cheese and butter). The PCR-RFLP test was perfo...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Agroprint Timisoara
2007-05-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | http://spasb.ro/index.php/spasb/article/view/1527 |
| Summary: | Beta-lactoglobulin (b-Lg) and kappa-casein (k-Cn) are two of the most important proteins in the mammals’ milk synthesized by the epithelial cells of the mammary glands. They play a crucial role in the milk quality and coagulation process (production of cheese and butter). The PCR-RFLP test was performed to distinguish the different alleles in a population of Romanian Black Spotted cattle, a dairy breed. Genetic polymorphism was detected by digestion with the endonucleases Hae III (b-Lg) and Hinf I (k-Cn), followed by electrophoresis in agarose high resolution gel stained with ethidium bromide. Fifty DNA samples from Romanian Black Spotted breed were analyzed for A and B variants. This simple PCR-RFLP test makes feasible the inclusion of b-Lg and k- Cn genotypes in breeding plans and cattle selection. |
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| ISSN: | 1841-9364 2344-4576 |