IDENTIFICATION OF BETA-LACTOGLOBULIN AND KAPPACASEIN GENOTYPES IN CATTLE

• Main Authors: R.A. VĂTĂŞESCU-BALCAN, S.E. GEORGESCU, MARIA ADINA MANEA, ANCA DINISCHIOTU, C.D. TESIO, MARIETA COSTACHE
• Format: Article
• Language: English
• Published: Agroprint Timisoara 2007-05-01
• Series: Scientific Papers Animal Science and Biotechnologies
• Subjects: kappa-casein; beta-lactoglobulin; genetic polymorphism; pcr-rflp test
• Online Access: http://spasb.ro/index.php/spasb/article/view/1527

Beta-lactoglobulin (b-Lg) and kappa-casein (k-Cn) are two of the most important proteins in the mammals’ milk synthesized by the epithelial cells of the mammary glands. They play a crucial role in the milk quality and coagulation process (production of cheese and butter). The PCR-RFLP test was performed to distinguish the different alleles in a population of Romanian Black Spotted cattle, a dairy breed. Genetic polymorphism was detected by digestion with the endonucleases Hae III (b-Lg) and Hinf I (k-Cn), followed by electrophoresis in agarose high resolution gel stained with ethidium bromide. Fifty DNA samples from Romanian Black Spotted breed were analyzed for A and B variants. This simple PCR-RFLP test makes feasible the inclusion of b-Lg and k- Cn genotypes in breeding plans and cattle selection.