Aldosterone rapidly activates p-PKC delta and GPR30 but suppresses p-PKC epsilon protein levels in rat kidney

Aldosterone rapidly activates p-PKC delta and GPR30 but suppresses p-PKC epsilon protein levels in rat kidney

Objectives. Aldosterone rapidly enhances protein kinase C (PKC) alpha and beta1 proteins in the rat kidney. The G protein-coupled receptor 30 (GPR30)-mediated PKC pathway is involved in the inhibition of the potassium channel in HEK-239 cells. GPR30 mediates rapid actions of aldosterone in vitro. Th...

Full description

Bibliographic Details
Main Authors: Eiam-Ong Somchit, Chaipipat Mookda, Manotham Krissanapong, Eiam-Ong Somchai
Format: Article
Language:English
Published: Sciendo 2019-07-01
Series:Endocrine Regulations
Subjects:
aldosterone
rapid action
p-pkc delta
p-pkc epsilon
gpr30
protein abundance
immunohistochemistry
rat kidney
Online Access:https://doi.org/10.2478/enr-2019-0016
Description
Summary:Objectives. Aldosterone rapidly enhances protein kinase C (PKC) alpha and beta1 proteins in the rat kidney. The G protein-coupled receptor 30 (GPR30)-mediated PKC pathway is involved in the inhibition of the potassium channel in HEK-239 cells. GPR30 mediates rapid actions of aldosterone in vitro. There are no reports available regarding the aldosterone action on other PKC isoforms and GPR30 proteins in vivo. The aim of the present study was to examine rapid actions of aldosterone on protein levels of phosphorylated PKC (p-PKC) delta, p-PKC epsilon, and GPR30 simultaneously in the rat kidney.
ISSN:1336-0329

Similar Items