Aldosterone rapidly activates p-PKC delta and GPR30 but suppresses p-PKC epsilon protein levels in rat kidney

• Main Authors: Eiam-Ong Somchit, Chaipipat Mookda, Manotham Krissanapong, Eiam-Ong Somchai
• Format: Article
• Language: English
• Published: Sciendo 2019-07-01
• Series: Endocrine Regulations
• Subjects: aldosterone; rapid action; p-pkc delta; p-pkc epsilon; gpr30; protein abundance; immunohistochemistry; rat kidney
• Online Access: https://doi.org/10.2478/enr-2019-0016

Objectives. Aldosterone rapidly enhances protein kinase C (PKC) alpha and beta1 proteins in the rat kidney. The G protein-coupled receptor 30 (GPR30)-mediated PKC pathway is involved in the inhibition of the potassium channel in HEK-239 cells. GPR30 mediates rapid actions of aldosterone in vitro. There are no reports available regarding the aldosterone action on other PKC isoforms and GPR30 proteins in vivo. The aim of the present study was to examine rapid actions of aldosterone on protein levels of phosphorylated PKC (p-PKC) delta, p-PKC epsilon, and GPR30 simultaneously in the rat kidney.