Benzene and Naphthalene Degrading Bacterial Communities in an Oil Sands Tailings Pond
Oil sands process-affected water (OSPW), produced by surface-mining of oil sands in Canada, is alkaline and contains high concentrations of salts, metals, naphthenic acids, and polycyclic aromatic compounds (PAHs). Residual hydrocarbon biodegradation occurs naturally, but little is known about the h...
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doaj-c5e4b2aa05e642cb8067e5b0d42c0a482020-11-24T23:21:34ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-09-01810.3389/fmicb.2017.01845272817Benzene and Naphthalene Degrading Bacterial Communities in an Oil Sands Tailings PondFauziah F. Rochman0Andriy Sheremet1Ivica Tamas2Ivica Tamas3Alireza Saidi-Mehrabad4Alireza Saidi-Mehrabad5Joong-Jae Kim6Xiaoli Dong7Xiaoli Dong8Christoph W. Sensen9Christoph W. Sensen10Lisa M. Gieg11Peter F. Dunfield12Department of Biological Sciences, University of Calgary, Calgary, AB, CanadaDepartment of Biological Sciences, University of Calgary, Calgary, AB, CanadaDepartment of Biological Sciences, University of Calgary, Calgary, AB, CanadaDepartment of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad, SerbiaDepartment of Biological Sciences, University of Calgary, Calgary, AB, CanadaDepartment of Biological Sciences, University of Alberta, Edmonton, AB, CanadaDepartment of Biological Sciences, University of Calgary, Calgary, AB, CanadaDepartment of Biochemistry and Molecular Biology in the Cumming School of Medicine, University of Calgary, Calgary, AB, CanadaDepartment of Geoscience, University of Calgary, Calgary, AB, CanadaDepartment of Biochemistry and Molecular Biology in the Cumming School of Medicine, University of Calgary, Calgary, AB, CanadaInstitute of Computational Biotechnology, Graz University of Technology, Graz, AustriaDepartment of Biological Sciences, University of Calgary, Calgary, AB, CanadaDepartment of Biological Sciences, University of Calgary, Calgary, AB, CanadaOil sands process-affected water (OSPW), produced by surface-mining of oil sands in Canada, is alkaline and contains high concentrations of salts, metals, naphthenic acids, and polycyclic aromatic compounds (PAHs). Residual hydrocarbon biodegradation occurs naturally, but little is known about the hydrocarbon-degrading microbial communities present in OSPW. In this study, aerobic oxidation of benzene and naphthalene in the surface layer of an oil sands tailings pond were measured. The potential oxidation rates were 4.3 μmol L−1 OSPW d−1 for benzene and 21.4 μmol L−1 OSPW d−1 for naphthalene. To identify benzene and naphthalene-degrading microbial communities, metagenomics was combined with stable isotope probing (SIP), high-throughput sequencing of 16S rRNA gene amplicons, and isolation of microbial strains. SIP using 13C-benzene and 13C-naphthalene detected strains of the genera Methyloversatilis and Zavarzinia as the main benzene degraders, while strains belonging to the family Chromatiaceae and the genus Thauera were the main naphthalene degraders. Metagenomic analysis revealed a diversity of genes encoding oxygenases active against aromatic compounds. Although these genes apparently belonged to many phylogenetically diverse taxa, only a few of these taxa were predominant in the SIP experiments. This suggested that many members of the community are adapted to consuming other aromatic compounds, or are active only under specific conditions. 16S rRNA gene sequence datasets have been submitted to the Sequence Read Archive (SRA) under accession number SRP109130. The Gold Study and Project submission ID number in Joint Genome Institute IMG/M for the metagenome is Gs0047444 and Gp0055765.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01845/fulloil sandstailings pondhydrocarbon degradationbenzenenaphthalenemetagenomics |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fauziah F. Rochman Andriy Sheremet Ivica Tamas Ivica Tamas Alireza Saidi-Mehrabad Alireza Saidi-Mehrabad Joong-Jae Kim Xiaoli Dong Xiaoli Dong Christoph W. Sensen Christoph W. Sensen Lisa M. Gieg Peter F. Dunfield |
spellingShingle |
Fauziah F. Rochman Andriy Sheremet Ivica Tamas Ivica Tamas Alireza Saidi-Mehrabad Alireza Saidi-Mehrabad Joong-Jae Kim Xiaoli Dong Xiaoli Dong Christoph W. Sensen Christoph W. Sensen Lisa M. Gieg Peter F. Dunfield Benzene and Naphthalene Degrading Bacterial Communities in an Oil Sands Tailings Pond Frontiers in Microbiology oil sands tailings pond hydrocarbon degradation benzene naphthalene metagenomics |
author_facet |
Fauziah F. Rochman Andriy Sheremet Ivica Tamas Ivica Tamas Alireza Saidi-Mehrabad Alireza Saidi-Mehrabad Joong-Jae Kim Xiaoli Dong Xiaoli Dong Christoph W. Sensen Christoph W. Sensen Lisa M. Gieg Peter F. Dunfield |
author_sort |
Fauziah F. Rochman |
title |
Benzene and Naphthalene Degrading Bacterial Communities in an Oil Sands Tailings Pond |
title_short |
Benzene and Naphthalene Degrading Bacterial Communities in an Oil Sands Tailings Pond |
title_full |
Benzene and Naphthalene Degrading Bacterial Communities in an Oil Sands Tailings Pond |
title_fullStr |
Benzene and Naphthalene Degrading Bacterial Communities in an Oil Sands Tailings Pond |
title_full_unstemmed |
Benzene and Naphthalene Degrading Bacterial Communities in an Oil Sands Tailings Pond |
title_sort |
benzene and naphthalene degrading bacterial communities in an oil sands tailings pond |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2017-09-01 |
description |
Oil sands process-affected water (OSPW), produced by surface-mining of oil sands in Canada, is alkaline and contains high concentrations of salts, metals, naphthenic acids, and polycyclic aromatic compounds (PAHs). Residual hydrocarbon biodegradation occurs naturally, but little is known about the hydrocarbon-degrading microbial communities present in OSPW. In this study, aerobic oxidation of benzene and naphthalene in the surface layer of an oil sands tailings pond were measured. The potential oxidation rates were 4.3 μmol L−1 OSPW d−1 for benzene and 21.4 μmol L−1 OSPW d−1 for naphthalene. To identify benzene and naphthalene-degrading microbial communities, metagenomics was combined with stable isotope probing (SIP), high-throughput sequencing of 16S rRNA gene amplicons, and isolation of microbial strains. SIP using 13C-benzene and 13C-naphthalene detected strains of the genera Methyloversatilis and Zavarzinia as the main benzene degraders, while strains belonging to the family Chromatiaceae and the genus Thauera were the main naphthalene degraders. Metagenomic analysis revealed a diversity of genes encoding oxygenases active against aromatic compounds. Although these genes apparently belonged to many phylogenetically diverse taxa, only a few of these taxa were predominant in the SIP experiments. This suggested that many members of the community are adapted to consuming other aromatic compounds, or are active only under specific conditions. 16S rRNA gene sequence datasets have been submitted to the Sequence Read Archive (SRA) under accession number SRP109130. The Gold Study and Project submission ID number in Joint Genome Institute IMG/M for the metagenome is Gs0047444 and Gp0055765. |
topic |
oil sands tailings pond hydrocarbon degradation benzene naphthalene metagenomics |
url |
http://journal.frontiersin.org/article/10.3389/fmicb.2017.01845/full |
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