The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging.
Polycomb Repressive Complex (PRC) 1 regulates the control of gene expression programs via chromatin structure reorganization. Through mutual exclusion, different PCGF members generate a variety of PRC1 complexes with potentially distinct cellular functions. In this context, the molecular function of...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2016-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4956247?pdf=render |
id |
doaj-c5fe9eb038904052b45e034b0a2b6f52 |
---|---|
record_format |
Article |
spelling |
doaj-c5fe9eb038904052b45e034b0a2b6f522020-11-24T22:11:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01117e015870010.1371/journal.pone.0158700The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging.Barbara DupretPamela VölkelXuefen Le BourhisPierre-Olivier AngrandPolycomb Repressive Complex (PRC) 1 regulates the control of gene expression programs via chromatin structure reorganization. Through mutual exclusion, different PCGF members generate a variety of PRC1 complexes with potentially distinct cellular functions. In this context, the molecular function of each of the PCGF family members remains elusive. The study of PCGF family member expression in zebrafish development and during caudal fin regeneration reveals that the zebrafish pcgf genes are subjected to different regulations and that all PRC1 complexes in terms of Pcgf subunit composition are not always present in the same tissues. To unveil the function of Pcgf1 in zebrafish, a mutant line was generated using the TALEN technology. Mutant pcgf1-/- fish are viable and fertile, but the growth rate at early developmental stages is reduced in absence of pcgf1 gene function and a significant number of pcgf1-/- fish show signs of premature aging. This first vertebrate model lacking Pcgf1 function shows that this Polycomb Group protein is involved in cell proliferation during early embryogenesis and establishes a link between epigenetics and aging.http://europepmc.org/articles/PMC4956247?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Barbara Dupret Pamela Völkel Xuefen Le Bourhis Pierre-Olivier Angrand |
spellingShingle |
Barbara Dupret Pamela Völkel Xuefen Le Bourhis Pierre-Olivier Angrand The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging. PLoS ONE |
author_facet |
Barbara Dupret Pamela Völkel Xuefen Le Bourhis Pierre-Olivier Angrand |
author_sort |
Barbara Dupret |
title |
The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging. |
title_short |
The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging. |
title_full |
The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging. |
title_fullStr |
The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging. |
title_full_unstemmed |
The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging. |
title_sort |
polycomb group protein pcgf1 is dispensable in zebrafish but involved in early growth and aging. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2016-01-01 |
description |
Polycomb Repressive Complex (PRC) 1 regulates the control of gene expression programs via chromatin structure reorganization. Through mutual exclusion, different PCGF members generate a variety of PRC1 complexes with potentially distinct cellular functions. In this context, the molecular function of each of the PCGF family members remains elusive. The study of PCGF family member expression in zebrafish development and during caudal fin regeneration reveals that the zebrafish pcgf genes are subjected to different regulations and that all PRC1 complexes in terms of Pcgf subunit composition are not always present in the same tissues. To unveil the function of Pcgf1 in zebrafish, a mutant line was generated using the TALEN technology. Mutant pcgf1-/- fish are viable and fertile, but the growth rate at early developmental stages is reduced in absence of pcgf1 gene function and a significant number of pcgf1-/- fish show signs of premature aging. This first vertebrate model lacking Pcgf1 function shows that this Polycomb Group protein is involved in cell proliferation during early embryogenesis and establishes a link between epigenetics and aging. |
url |
http://europepmc.org/articles/PMC4956247?pdf=render |
work_keys_str_mv |
AT barbaradupret thepolycombgroupproteinpcgf1isdispensableinzebrafishbutinvolvedinearlygrowthandaging AT pamelavolkel thepolycombgroupproteinpcgf1isdispensableinzebrafishbutinvolvedinearlygrowthandaging AT xuefenlebourhis thepolycombgroupproteinpcgf1isdispensableinzebrafishbutinvolvedinearlygrowthandaging AT pierreolivierangrand thepolycombgroupproteinpcgf1isdispensableinzebrafishbutinvolvedinearlygrowthandaging AT barbaradupret polycombgroupproteinpcgf1isdispensableinzebrafishbutinvolvedinearlygrowthandaging AT pamelavolkel polycombgroupproteinpcgf1isdispensableinzebrafishbutinvolvedinearlygrowthandaging AT xuefenlebourhis polycombgroupproteinpcgf1isdispensableinzebrafishbutinvolvedinearlygrowthandaging AT pierreolivierangrand polycombgroupproteinpcgf1isdispensableinzebrafishbutinvolvedinearlygrowthandaging |
_version_ |
1725805630578491392 |