Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions
Acute brain slice preparation is a powerful experimental model for investigating the characteristics of synaptic function in the brain. Although brain tissue is usually cut at ice-cold temperature (CT) to facilitate slicing and avoid neuronal damage, exposure to CT causes molecular and architectural...
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doaj-c600924fb9664d95874df254deea8e902020-11-25T03:51:40ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022020-03-011410.3389/fncel.2020.00063515561Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic FunctionsKohgaku Eguchi0Philipp Velicky1Elena Hollergschwandtner2Makoto Itakura3Yugo Fukazawa4Johann Georg Danzl5Ryuichi Shigemoto6Institute of Science and Technology Austria (IST Austria), Klosterneuburg, AustriaInstitute of Science and Technology Austria (IST Austria), Klosterneuburg, AustriaInstitute of Science and Technology Austria (IST Austria), Klosterneuburg, AustriaDepartment of Biochemistry, Kitasato University School of Medicine, Sagamihara-shi, JapanDepartment of Brain Structure and Function, Research Center for Child Mental Development, Faculty of Medical Sciences, University of Fukui, Fukui, JapanInstitute of Science and Technology Austria (IST Austria), Klosterneuburg, AustriaInstitute of Science and Technology Austria (IST Austria), Klosterneuburg, AustriaAcute brain slice preparation is a powerful experimental model for investigating the characteristics of synaptic function in the brain. Although brain tissue is usually cut at ice-cold temperature (CT) to facilitate slicing and avoid neuronal damage, exposure to CT causes molecular and architectural changes of synapses. To address these issues, we investigated ultrastructural and electrophysiological features of synapses in mouse acute cerebellar slices prepared at ice-cold and physiological temperature (PT). In the slices prepared at CT, we found significant spine loss and reconstruction, synaptic vesicle rearrangement and decrease in synaptic proteins, all of which were not detected in slices prepared at PT. Consistent with these structural findings, slices prepared at PT showed higher release probability. Furthermore, preparation at PT allows electrophysiological recording immediately after slicing resulting in higher detectability of long-term depression (LTD) after motor learning compared with that at CT. These results indicate substantial advantages of the slice preparation at PT for investigating synaptic functions in different physiological conditions.https://www.frontiersin.org/article/10.3389/fncel.2020.00063/fullacute brain slicessynaptic transmissionsynaptic plasticitycerebellumsuper-resolution microscopyelectron microscopy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kohgaku Eguchi Philipp Velicky Elena Hollergschwandtner Makoto Itakura Yugo Fukazawa Johann Georg Danzl Ryuichi Shigemoto |
spellingShingle |
Kohgaku Eguchi Philipp Velicky Elena Hollergschwandtner Makoto Itakura Yugo Fukazawa Johann Georg Danzl Ryuichi Shigemoto Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions Frontiers in Cellular Neuroscience acute brain slices synaptic transmission synaptic plasticity cerebellum super-resolution microscopy electron microscopy |
author_facet |
Kohgaku Eguchi Philipp Velicky Elena Hollergschwandtner Makoto Itakura Yugo Fukazawa Johann Georg Danzl Ryuichi Shigemoto |
author_sort |
Kohgaku Eguchi |
title |
Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions |
title_short |
Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions |
title_full |
Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions |
title_fullStr |
Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions |
title_full_unstemmed |
Advantages of Acute Brain Slices Prepared at Physiological Temperature in the Characterization of Synaptic Functions |
title_sort |
advantages of acute brain slices prepared at physiological temperature in the characterization of synaptic functions |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular Neuroscience |
issn |
1662-5102 |
publishDate |
2020-03-01 |
description |
Acute brain slice preparation is a powerful experimental model for investigating the characteristics of synaptic function in the brain. Although brain tissue is usually cut at ice-cold temperature (CT) to facilitate slicing and avoid neuronal damage, exposure to CT causes molecular and architectural changes of synapses. To address these issues, we investigated ultrastructural and electrophysiological features of synapses in mouse acute cerebellar slices prepared at ice-cold and physiological temperature (PT). In the slices prepared at CT, we found significant spine loss and reconstruction, synaptic vesicle rearrangement and decrease in synaptic proteins, all of which were not detected in slices prepared at PT. Consistent with these structural findings, slices prepared at PT showed higher release probability. Furthermore, preparation at PT allows electrophysiological recording immediately after slicing resulting in higher detectability of long-term depression (LTD) after motor learning compared with that at CT. These results indicate substantial advantages of the slice preparation at PT for investigating synaptic functions in different physiological conditions. |
topic |
acute brain slices synaptic transmission synaptic plasticity cerebellum super-resolution microscopy electron microscopy |
url |
https://www.frontiersin.org/article/10.3389/fncel.2020.00063/full |
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