Impact of Vegetative Treatment Systems on Multiple Measures of Antibiotic Resistance in Agricultural Wastewater
Wastewater is an important vector of antibiotic resistant bacteria and antibiotic resistance genes (ARB/G). While there is broad agreement that ARB/G from agricultural (ag) wastewaters can be transported through the environment and may contribute to untreatable infectious disease in humans and anima...
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doaj-c613009f01d745d89cfa69c9378ad1922020-11-24T21:02:20ZengMDPI AGInternational Journal of Environmental Research and Public Health1660-46012018-06-01157129510.3390/ijerph15071295ijerph15071295Impact of Vegetative Treatment Systems on Multiple Measures of Antibiotic Resistance in Agricultural WastewaterLisa M. Durso0Daniel N. Miller1Christopher G. Henry2United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Agroecosystem Management Research Unit, 251 Filley Hall, UNL East Campus Lincoln, Lincoln, NE 68583, USAUnited States Department of Agriculture (USDA), Agricultural Research Service (ARS), Agroecosystem Management Research Unit, 251 Filley Hall, UNL East Campus Lincoln, Lincoln, NE 68583, USARice Research and Extension Center, University of Arkansas, Stuttgart, AR 72160, USAWastewater is an important vector of antibiotic resistant bacteria and antibiotic resistance genes (ARB/G). While there is broad agreement that ARB/G from agricultural (ag) wastewaters can be transported through the environment and may contribute to untreatable infectious disease in humans and animals, there remain large knowledge gaps surrounding applied details on the types and amounts of ARB/G associated with different agricultural wastewater treatment options and different ag production systems. This study evaluates a vegetative treatment system (VTS) built to treat the wastewater from a beef cattle feedlot. Samples were collected for three years, and plated on multiple media types to enumerate tetracycline and cefotaxime-resistant bacteria. Enterobacteriaceae isolates (n = 822) were characterized for carriage of tetracycline resistance genes, and E. coli isolates (n = 673) were phenotyped to determine multi-drug resistance (MDR) profiles. Tetracycline resistance in feedlot runoff wastewater was 2-to-3 orders of magnitude higher compared to rainfall runoff from the VTS fields, indicating efficacy of the VTA for reducing ARB over time following wastewater application. Clear differences in MDR profiles were observed based on the specific media on which a sample was plated. This result highlights the importance of method, especially in the context of isolate-based surveillance and monitoring of ARB in agricultural wastewaters.http://www.mdpi.com/1660-4601/15/7/1295antibiotic resistanceagriculturewastewatermanurecattleantibiotic resistant bacteriaantibiotic resistance genetetracycline resistance geneenvironmentvegetative treatment system |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lisa M. Durso Daniel N. Miller Christopher G. Henry |
spellingShingle |
Lisa M. Durso Daniel N. Miller Christopher G. Henry Impact of Vegetative Treatment Systems on Multiple Measures of Antibiotic Resistance in Agricultural Wastewater International Journal of Environmental Research and Public Health antibiotic resistance agriculture wastewater manure cattle antibiotic resistant bacteria antibiotic resistance gene tetracycline resistance gene environment vegetative treatment system |
author_facet |
Lisa M. Durso Daniel N. Miller Christopher G. Henry |
author_sort |
Lisa M. Durso |
title |
Impact of Vegetative Treatment Systems on Multiple Measures of Antibiotic Resistance in Agricultural Wastewater |
title_short |
Impact of Vegetative Treatment Systems on Multiple Measures of Antibiotic Resistance in Agricultural Wastewater |
title_full |
Impact of Vegetative Treatment Systems on Multiple Measures of Antibiotic Resistance in Agricultural Wastewater |
title_fullStr |
Impact of Vegetative Treatment Systems on Multiple Measures of Antibiotic Resistance in Agricultural Wastewater |
title_full_unstemmed |
Impact of Vegetative Treatment Systems on Multiple Measures of Antibiotic Resistance in Agricultural Wastewater |
title_sort |
impact of vegetative treatment systems on multiple measures of antibiotic resistance in agricultural wastewater |
publisher |
MDPI AG |
series |
International Journal of Environmental Research and Public Health |
issn |
1660-4601 |
publishDate |
2018-06-01 |
description |
Wastewater is an important vector of antibiotic resistant bacteria and antibiotic resistance genes (ARB/G). While there is broad agreement that ARB/G from agricultural (ag) wastewaters can be transported through the environment and may contribute to untreatable infectious disease in humans and animals, there remain large knowledge gaps surrounding applied details on the types and amounts of ARB/G associated with different agricultural wastewater treatment options and different ag production systems. This study evaluates a vegetative treatment system (VTS) built to treat the wastewater from a beef cattle feedlot. Samples were collected for three years, and plated on multiple media types to enumerate tetracycline and cefotaxime-resistant bacteria. Enterobacteriaceae isolates (n = 822) were characterized for carriage of tetracycline resistance genes, and E. coli isolates (n = 673) were phenotyped to determine multi-drug resistance (MDR) profiles. Tetracycline resistance in feedlot runoff wastewater was 2-to-3 orders of magnitude higher compared to rainfall runoff from the VTS fields, indicating efficacy of the VTA for reducing ARB over time following wastewater application. Clear differences in MDR profiles were observed based on the specific media on which a sample was plated. This result highlights the importance of method, especially in the context of isolate-based surveillance and monitoring of ARB in agricultural wastewaters. |
topic |
antibiotic resistance agriculture wastewater manure cattle antibiotic resistant bacteria antibiotic resistance gene tetracycline resistance gene environment vegetative treatment system |
url |
http://www.mdpi.com/1660-4601/15/7/1295 |
work_keys_str_mv |
AT lisamdurso impactofvegetativetreatmentsystemsonmultiplemeasuresofantibioticresistanceinagriculturalwastewater AT danielnmiller impactofvegetativetreatmentsystemsonmultiplemeasuresofantibioticresistanceinagriculturalwastewater AT christopherghenry impactofvegetativetreatmentsystemsonmultiplemeasuresofantibioticresistanceinagriculturalwastewater |
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1716775715415785472 |