Antihypertensive and renal protective effect of Shunaoxin pill combined with captopril on spontaneous hypertension rats
Introduction: According to previous reports, hypertension has become the most common chronic disease in the world. Captopril, an angiotensin-converting enzyme inhibitor, has been widely used for the therapy of arterial hypertension and cardiovascular diseases therapy. Besides, Shunaoxin pill (SNX) a...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2020-05-01
|
Series: | Biomedicine & Pharmacotherapy |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332220301670 |
id |
doaj-c6163f7b1f574497953c5edde80f3e8b |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shuli Man Li Yang Hao Xiang Guanyu Lu Yijia Wang Changxiao Liu Wenyuan Gao |
spellingShingle |
Shuli Man Li Yang Hao Xiang Guanyu Lu Yijia Wang Changxiao Liu Wenyuan Gao Antihypertensive and renal protective effect of Shunaoxin pill combined with captopril on spontaneous hypertension rats Biomedicine & Pharmacotherapy Shunaoxin pill Captopril Combinatorial treatment Antihypertension Renoprotection |
author_facet |
Shuli Man Li Yang Hao Xiang Guanyu Lu Yijia Wang Changxiao Liu Wenyuan Gao |
author_sort |
Shuli Man |
title |
Antihypertensive and renal protective effect of Shunaoxin pill combined with captopril on spontaneous hypertension rats |
title_short |
Antihypertensive and renal protective effect of Shunaoxin pill combined with captopril on spontaneous hypertension rats |
title_full |
Antihypertensive and renal protective effect of Shunaoxin pill combined with captopril on spontaneous hypertension rats |
title_fullStr |
Antihypertensive and renal protective effect of Shunaoxin pill combined with captopril on spontaneous hypertension rats |
title_full_unstemmed |
Antihypertensive and renal protective effect of Shunaoxin pill combined with captopril on spontaneous hypertension rats |
title_sort |
antihypertensive and renal protective effect of shunaoxin pill combined with captopril on spontaneous hypertension rats |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2020-05-01 |
description |
Introduction: According to previous reports, hypertension has become the most common chronic disease in the world. Captopril, an angiotensin-converting enzyme inhibitor, has been widely used for the therapy of arterial hypertension and cardiovascular diseases therapy. Besides, Shunaoxin pill (SNX) as a traditional Chinese prescription showed antihypertensive effect in our previous research. Objective: This study means to investigate whether SNX combining with captopril could show antihypertensive and renal protective effects on spontaneous hypertension rats (SHRs). Methods: SHRs were randomly assigned to four treatment groups, including non-treated group, captopril, SNX, and captopril + SNX-treated groups. Their body weight and systolic blood pressure (SBP) were measured weekly. Histopathological examination was analyzed through Masson staining and hematoxylin and eosin staining. Biochemical analyses, ELISA, and western blot were used to analyze their combining mechanism. Results: In this experiment, this combinatorial therapy significantly reduced aortic wall thickness, increased the content of NO, NOS and eNOS, decreased the content of bradykinin and endothelin 1(ET-1), and regulated the levels of TG, TC and HDLC back to normal, which suggested they could induce vasodilation and lower blood pressure. Meanwhile, histological examination alleviated that captopril + SNX remarkably inhibited renal injury, including tubular disorder, inflammatory cell infiltration and fibrosis. They down-regulated the serum levels of BUN and Cr, protein expression of IL-1β, NF-κB, Bax, Cyt c, caspase 3, 8 and 9 in kidney tissues and significantly increased the levels of Bcl-2 in kidney tissues compared with monotherapy group. Conclusion: The combinatorial treatment of SNX and captopril lowered blood pressure through adjusting NO/NOS, ET-1 and dyslipidemia profile. Furthermore, this treatment alleviated the kidney damage via reducing the release of inflammatory factors and the expression of apoptotic markers. Therefore, these results provided a rationale for future clinical use of SNX combined with captopril in antihypertensive and protecting renal functions in hypertension. |
topic |
Shunaoxin pill Captopril Combinatorial treatment Antihypertension Renoprotection |
url |
http://www.sciencedirect.com/science/article/pii/S0753332220301670 |
work_keys_str_mv |
AT shuliman antihypertensiveandrenalprotectiveeffectofshunaoxinpillcombinedwithcaptoprilonspontaneoushypertensionrats AT liyang antihypertensiveandrenalprotectiveeffectofshunaoxinpillcombinedwithcaptoprilonspontaneoushypertensionrats AT haoxiang antihypertensiveandrenalprotectiveeffectofshunaoxinpillcombinedwithcaptoprilonspontaneoushypertensionrats AT guanyulu antihypertensiveandrenalprotectiveeffectofshunaoxinpillcombinedwithcaptoprilonspontaneoushypertensionrats AT yijiawang antihypertensiveandrenalprotectiveeffectofshunaoxinpillcombinedwithcaptoprilonspontaneoushypertensionrats AT changxiaoliu antihypertensiveandrenalprotectiveeffectofshunaoxinpillcombinedwithcaptoprilonspontaneoushypertensionrats AT wenyuangao antihypertensiveandrenalprotectiveeffectofshunaoxinpillcombinedwithcaptoprilonspontaneoushypertensionrats |
_version_ |
1721435148916883456 |
spelling |
doaj-c6163f7b1f574497953c5edde80f3e8b2021-05-20T07:40:52ZengElsevierBiomedicine & Pharmacotherapy0753-33222020-05-01125109977Antihypertensive and renal protective effect of Shunaoxin pill combined with captopril on spontaneous hypertension ratsShuli Man0Li Yang1Hao Xiang2Guanyu Lu3Yijia Wang4Changxiao Liu5Wenyuan Gao6State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, China; Corresponding author at: College of Biotechnology, Tianjin University of Science & Technology, TEDA 13th street, Tianjin, 300457, China.State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, National and Local United Engineering Lab of Metabolic Control Fermentation Technology, China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, ChinaTianjin International School, 4-1 Sishui Rd, Hexi District, Tianjin, 300222, ChinaThe State Key Laboratories of Pharmacodynamics and Pharmacokinetics, Tianjin, 300193, ChinaTianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China; Corresponding author at: School of Pharmaceutical Science and Technology, Tianjin University, Weijin Road, Tianjin, 300072, China.Introduction: According to previous reports, hypertension has become the most common chronic disease in the world. Captopril, an angiotensin-converting enzyme inhibitor, has been widely used for the therapy of arterial hypertension and cardiovascular diseases therapy. Besides, Shunaoxin pill (SNX) as a traditional Chinese prescription showed antihypertensive effect in our previous research. Objective: This study means to investigate whether SNX combining with captopril could show antihypertensive and renal protective effects on spontaneous hypertension rats (SHRs). Methods: SHRs were randomly assigned to four treatment groups, including non-treated group, captopril, SNX, and captopril + SNX-treated groups. Their body weight and systolic blood pressure (SBP) were measured weekly. Histopathological examination was analyzed through Masson staining and hematoxylin and eosin staining. Biochemical analyses, ELISA, and western blot were used to analyze their combining mechanism. Results: In this experiment, this combinatorial therapy significantly reduced aortic wall thickness, increased the content of NO, NOS and eNOS, decreased the content of bradykinin and endothelin 1(ET-1), and regulated the levels of TG, TC and HDLC back to normal, which suggested they could induce vasodilation and lower blood pressure. Meanwhile, histological examination alleviated that captopril + SNX remarkably inhibited renal injury, including tubular disorder, inflammatory cell infiltration and fibrosis. They down-regulated the serum levels of BUN and Cr, protein expression of IL-1β, NF-κB, Bax, Cyt c, caspase 3, 8 and 9 in kidney tissues and significantly increased the levels of Bcl-2 in kidney tissues compared with monotherapy group. Conclusion: The combinatorial treatment of SNX and captopril lowered blood pressure through adjusting NO/NOS, ET-1 and dyslipidemia profile. Furthermore, this treatment alleviated the kidney damage via reducing the release of inflammatory factors and the expression of apoptotic markers. Therefore, these results provided a rationale for future clinical use of SNX combined with captopril in antihypertensive and protecting renal functions in hypertension.http://www.sciencedirect.com/science/article/pii/S0753332220301670Shunaoxin pillCaptoprilCombinatorial treatmentAntihypertensionRenoprotection |