Investigating the antibacterial potential of agarose nanoparticles synthesized by nanoprecipitation technology
Herein, an effort was made to investigate the antibacterial potential of agarose nanoparticles (ANPs) and poly(quaternary ammonium) modified ANPs (mANPs) against Escherichia coli (gram-negative bacterium) and Staphylococcus aureus (gram positive bacterium) in liquid systems as well as on agar plates...
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doaj-c620d70808354084b145666ae6d2d03a2021-09-05T13:59:43ZengSciendoPolish Journal of Chemical Technology1899-47412016-06-0118291210.1515/pjct-2016-0022pjct-2016-0022Investigating the antibacterial potential of agarose nanoparticles synthesized by nanoprecipitation technologySatar Rukhsana0Iizhar Syed Ahmed1Rasool Mahmood2Pushparaj Peter Natesan3Ansari Shakeel Ahmed4Ibn Sina National College for Medical Sciences, Department of Biochemistry, Jeddah-21418, Kingdom of Saudi ArabiaIbn Sina National College of Medical Studies, Department of Pharmaceutical Sciences, Jeddah-21418, Kingdom of Saudi ArabiaKing Abdulaziz University, Center of Excellence in Genomic and Medicine Research, Jeddah-21589, Kingdom of Saudi ArabiaKing Abdulaziz University, Center of Excellence in Genomic and Medicine Research, Jeddah-21589, Kingdom of Saudi ArabiaKing Abdulaziz University, Center of Excellence in Genomic and Medicine Research, Jeddah-21589, Kingdom of Saudi ArabiaHerein, an effort was made to investigate the antibacterial potential of agarose nanoparticles (ANPs) and poly(quaternary ammonium) modified ANPs (mANPs) against Escherichia coli (gram-negative bacterium) and Staphylococcus aureus (gram positive bacterium) in liquid systems as well as on agar plates. ANPs were synthesized by nanoprecipitation technology and characterized by XRD, TEM, TGA, DTA and DLS. The particle size estimated was 30 nm while atomic force microscopy was used to observe the interaction of ligand on ANPs. Antimicrobial characterization was monitored by colony forming units (CFU) as a function of ANPs concentration on agar plates. It was observed that ANPs showed 15 × 109/ml CFU after 24 hours of incubation at 20 mM ANPs concentration while the modified ANPs exhibited 21 × 109/ml CFU under similar incubation conditions. Moreover, zone of inhibition (ZOI) was 2.9 and 3.8 cm, respectively for E. coli by ANPs at 0.2 and 0.4 mM, respectively while it was 3.2 and 3.8 cm respectively by modified ANPs under similar conditions. Similarly, ZOI for S. aureus by ANPs at 0.2 and 0.4 mM was observed at 3.1 and 4.0 cm, respectively, while these values were 3.5 and 4.1 cm, respectively for modified ANPs under similar incubation conditions.https://doi.org/10.1515/pjct-2016-0022agarose nanoparticlesantibacterial propertiessurface modificationnanotechnology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Satar Rukhsana Iizhar Syed Ahmed Rasool Mahmood Pushparaj Peter Natesan Ansari Shakeel Ahmed |
spellingShingle |
Satar Rukhsana Iizhar Syed Ahmed Rasool Mahmood Pushparaj Peter Natesan Ansari Shakeel Ahmed Investigating the antibacterial potential of agarose nanoparticles synthesized by nanoprecipitation technology Polish Journal of Chemical Technology agarose nanoparticles antibacterial properties surface modification nanotechnology |
author_facet |
Satar Rukhsana Iizhar Syed Ahmed Rasool Mahmood Pushparaj Peter Natesan Ansari Shakeel Ahmed |
author_sort |
Satar Rukhsana |
title |
Investigating the antibacterial potential of agarose nanoparticles synthesized by nanoprecipitation technology |
title_short |
Investigating the antibacterial potential of agarose nanoparticles synthesized by nanoprecipitation technology |
title_full |
Investigating the antibacterial potential of agarose nanoparticles synthesized by nanoprecipitation technology |
title_fullStr |
Investigating the antibacterial potential of agarose nanoparticles synthesized by nanoprecipitation technology |
title_full_unstemmed |
Investigating the antibacterial potential of agarose nanoparticles synthesized by nanoprecipitation technology |
title_sort |
investigating the antibacterial potential of agarose nanoparticles synthesized by nanoprecipitation technology |
publisher |
Sciendo |
series |
Polish Journal of Chemical Technology |
issn |
1899-4741 |
publishDate |
2016-06-01 |
description |
Herein, an effort was made to investigate the antibacterial potential of agarose nanoparticles (ANPs) and poly(quaternary ammonium) modified ANPs (mANPs) against Escherichia coli (gram-negative bacterium) and Staphylococcus aureus (gram positive bacterium) in liquid systems as well as on agar plates. ANPs were synthesized by nanoprecipitation technology and characterized by XRD, TEM, TGA, DTA and DLS. The particle size estimated was 30 nm while atomic force microscopy was used to observe the interaction of ligand on ANPs. Antimicrobial characterization was monitored by colony forming units (CFU) as a function of ANPs concentration on agar plates. It was observed that ANPs showed 15 × 109/ml CFU after 24 hours of incubation at 20 mM ANPs concentration while the modified ANPs exhibited 21 × 109/ml CFU under similar incubation conditions. Moreover, zone of inhibition (ZOI) was 2.9 and 3.8 cm, respectively for E. coli by ANPs at 0.2 and 0.4 mM, respectively while it was 3.2 and 3.8 cm respectively by modified ANPs under similar conditions. Similarly, ZOI for S. aureus by ANPs at 0.2 and 0.4 mM was observed at 3.1 and 4.0 cm, respectively, while these values were 3.5 and 4.1 cm, respectively for modified ANPs under similar incubation conditions. |
topic |
agarose nanoparticles antibacterial properties surface modification nanotechnology |
url |
https://doi.org/10.1515/pjct-2016-0022 |
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