Evolvulus alsinoides methanolic extract triggers apoptosis in HepG2 cells
Objective: The objective of the present study was to evaluate the cytotoxic potentials of Evolvulus alsinoides in human hepatoma HepG2 cells. Materials and Methods: HepG2 cells were treated with methanolic extract of E. alsinoides at 20, 40 and 80 µg/ml for 24 hr and cytotoxic effect was analyzed by...
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Mashhad University of Medical Sciences
2018-11-01
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doaj-c65ea70e52124b08a6f05b8026438f4d2020-11-25T01:30:08ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492018-11-018650451210.22038/ajp.2018.1128511285Evolvulus alsinoides methanolic extract triggers apoptosis in HepG2 cellsInduja MP0Ezhilarasan Devaraj1Ashok Vardhan Nandigam2Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, IndiaSaveetha Institute of Medical and Technical SciencesBiomedical Research Unit and Laboratory Animal Centre, Saveetha Dental College, Chennai, Tamil Nadu, India – 600 077Objective: The objective of the present study was to evaluate the cytotoxic potentials of Evolvulus alsinoides in human hepatoma HepG2 cells. Materials and Methods: HepG2 cells were treated with methanolic extract of E. alsinoides at 20, 40 and 80 µg/ml for 24 hr and cytotoxic effect was analyzed by MTT assay. The apoptosis rate was investigated by Hoechst 33342 and annexin V/propidium iodide staining. Mitochondrial membrane potential was evaluated by rhodamine staining. Also, the expression of catenin – β 1 protein was analyzed by western blotting. Results: E. alsinoides methanolic extract treatment caused significant cytotoxicity in HepG2 cells in a concentration-dependent manner.Dual staining assayconfirmed the presence of early and late apoptotic cells only in extract-treated groups. Plant extract treatment also caused nuclear fragmentation and chromatin condensation in HepG2 cells. Mitochondrial membrane potential also reduced upon E. alsinoides treatments.This treatment also modulated the catenin – β 1 protein expression. Conclusion:In this study, we demonstrated theproapoptotic potential E. alsinoides in HepG2 cells; thus, this plant may be beneficial in the treatment of liver cancer.http://ajp.mums.ac.ir/article_11285_974bd05433edb442ffcac51278b29472.pdfApoptosisDual stainingCytotoxicityProliferationCatenin – β 1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Induja MP Ezhilarasan Devaraj Ashok Vardhan Nandigam |
spellingShingle |
Induja MP Ezhilarasan Devaraj Ashok Vardhan Nandigam Evolvulus alsinoides methanolic extract triggers apoptosis in HepG2 cells Avicenna Journal of Phytomedicine Apoptosis Dual staining Cytotoxicity Proliferation Catenin – β 1 |
author_facet |
Induja MP Ezhilarasan Devaraj Ashok Vardhan Nandigam |
author_sort |
Induja MP |
title |
Evolvulus alsinoides methanolic extract triggers apoptosis in HepG2 cells |
title_short |
Evolvulus alsinoides methanolic extract triggers apoptosis in HepG2 cells |
title_full |
Evolvulus alsinoides methanolic extract triggers apoptosis in HepG2 cells |
title_fullStr |
Evolvulus alsinoides methanolic extract triggers apoptosis in HepG2 cells |
title_full_unstemmed |
Evolvulus alsinoides methanolic extract triggers apoptosis in HepG2 cells |
title_sort |
evolvulus alsinoides methanolic extract triggers apoptosis in hepg2 cells |
publisher |
Mashhad University of Medical Sciences |
series |
Avicenna Journal of Phytomedicine |
issn |
2228-7930 2228-7949 |
publishDate |
2018-11-01 |
description |
Objective: The objective of the present study was to evaluate the cytotoxic potentials of Evolvulus alsinoides in human hepatoma HepG2 cells. Materials and Methods: HepG2 cells were treated with methanolic extract of E. alsinoides at 20, 40 and 80 µg/ml for 24 hr and cytotoxic effect was analyzed by MTT assay. The apoptosis rate was investigated by Hoechst 33342 and annexin V/propidium iodide staining. Mitochondrial membrane potential was evaluated by rhodamine staining. Also, the expression of catenin – β 1 protein was analyzed by western blotting. Results: E. alsinoides methanolic extract treatment caused significant cytotoxicity in HepG2 cells in a concentration-dependent manner.Dual staining assayconfirmed the presence of early and late apoptotic cells only in extract-treated groups. Plant extract treatment also caused nuclear fragmentation and chromatin condensation in HepG2 cells. Mitochondrial membrane potential also reduced upon E. alsinoides treatments.This treatment also modulated the catenin – β 1 protein expression. Conclusion:In this study, we demonstrated theproapoptotic potential E. alsinoides in HepG2 cells; thus, this plant may be beneficial in the treatment of liver cancer. |
topic |
Apoptosis Dual staining Cytotoxicity Proliferation Catenin – β 1 |
url |
http://ajp.mums.ac.ir/article_11285_974bd05433edb442ffcac51278b29472.pdf |
work_keys_str_mv |
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