Amino acids signatures of distance-related surgical margins of oral squamous cell carcinomaResearch in context
Background: Histological assessment of resected margins has some drawbacks. We therefore aimed to identify a panel of metabolic markers for evaluating the surgical margins of oral squamous cell carcinoma during surgery. Methods: A total of 28 case of OSCC samples were enrolled in the study. Gas chro...
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Format: | Article |
Language: | English |
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Elsevier
2019-10-01
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Series: | EBioMedicine |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2352396419306693 |
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doaj-c6669b6a9f5b41a989c2ffda2ec41edb |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xi-Hu Yang Xiao-Xin Zhang Yue Jing Liang Ding Yong Fu Shuai Wang Shi-qi Hu Lei Zhang Xiao-Feng Huang Yan-Hong Ni Qin-Gang Hu |
spellingShingle |
Xi-Hu Yang Xiao-Xin Zhang Yue Jing Liang Ding Yong Fu Shuai Wang Shi-qi Hu Lei Zhang Xiao-Feng Huang Yan-Hong Ni Qin-Gang Hu Amino acids signatures of distance-related surgical margins of oral squamous cell carcinomaResearch in context EBioMedicine |
author_facet |
Xi-Hu Yang Xiao-Xin Zhang Yue Jing Liang Ding Yong Fu Shuai Wang Shi-qi Hu Lei Zhang Xiao-Feng Huang Yan-Hong Ni Qin-Gang Hu |
author_sort |
Xi-Hu Yang |
title |
Amino acids signatures of distance-related surgical margins of oral squamous cell carcinomaResearch in context |
title_short |
Amino acids signatures of distance-related surgical margins of oral squamous cell carcinomaResearch in context |
title_full |
Amino acids signatures of distance-related surgical margins of oral squamous cell carcinomaResearch in context |
title_fullStr |
Amino acids signatures of distance-related surgical margins of oral squamous cell carcinomaResearch in context |
title_full_unstemmed |
Amino acids signatures of distance-related surgical margins of oral squamous cell carcinomaResearch in context |
title_sort |
amino acids signatures of distance-related surgical margins of oral squamous cell carcinomaresearch in context |
publisher |
Elsevier |
series |
EBioMedicine |
issn |
2352-3964 |
publishDate |
2019-10-01 |
description |
Background: Histological assessment of resected margins has some drawbacks. We therefore aimed to identify a panel of metabolic markers for evaluating the surgical margins of oral squamous cell carcinoma during surgery. Methods: A total of 28 case of OSCC samples were enrolled in the study. Gas chromatography-mass spectrometry based untargeted metabolic analysis was employed to acquire the metabolic perturbation of the distance-related surgical margins in the development group. The acquired MS data were then subjected to univariate and multivariate analysis by MetaboAnalyst. Ultra–high performance liquid chromatography–tandem mass spectrometerbased targeted metabolomics for quantitative analysis of the validation group was performed to verify the results of the development group. Another 60 OSCC patients with dysplastic surgical margins were used to further validate the results of the development group by immunohistochemical examination of key enzyme expression, and correlate them with clinicopathological parameters and clinical outcomes. Findings: We finally identified 4 amino acids as negative margin markers, and 6 amino acids as dysplastic margin markers. IHC analysis showed that asparagine synthetase positive expression in dysplastic surgical margins and its higher expression was correlated with tumor recurrence and local relapse-free survival. Interpretations: We developed a panel of metabolic molecular markers to supplement the evaluation of negative and dysplastic margins. Fund: This study was supported by Nanjing Municipal Key Medical Laboratory Constructional Project Funding (Since 2012); Center of Nanjing Clinical Medicine Tumor (Since 2014). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Keywords: Oral squamous cell carcinoma, Amino acids metabolomics, Surgical margins, Asparagine synthetase |
url |
http://www.sciencedirect.com/science/article/pii/S2352396419306693 |
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doaj-c6669b6a9f5b41a989c2ffda2ec41edb2020-11-24T21:50:06ZengElsevierEBioMedicine2352-39642019-10-01488191Amino acids signatures of distance-related surgical margins of oral squamous cell carcinomaResearch in contextXi-Hu Yang0Xiao-Xin Zhang1Yue Jing2Liang Ding3Yong Fu4Shuai Wang5Shi-qi Hu6Lei Zhang7Xiao-Feng Huang8Yan-Hong Ni9Qin-Gang Hu10Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, China; Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, ChinaCentral Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, ChinaCentral Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, ChinaCentral Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, ChinaDepartment of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, ChinaCentral Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, China; Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, ChinaDepartment of Oral Pathology, Stomatological hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, ChinaDepartment of Oral Pathology, Stomatological hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, ChinaDepartment of Oral Pathology, Stomatological hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, ChinaCentral Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, China; Corresponding author: Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, China.Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, China; Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210000, China; Corresponding author: Department of Oral and Maxillofacial Surgery, Stomatological Hospital, School of medicine, Nanjing University, Nanjing, Jiangsu 210000, China.Background: Histological assessment of resected margins has some drawbacks. We therefore aimed to identify a panel of metabolic markers for evaluating the surgical margins of oral squamous cell carcinoma during surgery. Methods: A total of 28 case of OSCC samples were enrolled in the study. Gas chromatography-mass spectrometry based untargeted metabolic analysis was employed to acquire the metabolic perturbation of the distance-related surgical margins in the development group. The acquired MS data were then subjected to univariate and multivariate analysis by MetaboAnalyst. Ultra–high performance liquid chromatography–tandem mass spectrometerbased targeted metabolomics for quantitative analysis of the validation group was performed to verify the results of the development group. Another 60 OSCC patients with dysplastic surgical margins were used to further validate the results of the development group by immunohistochemical examination of key enzyme expression, and correlate them with clinicopathological parameters and clinical outcomes. Findings: We finally identified 4 amino acids as negative margin markers, and 6 amino acids as dysplastic margin markers. IHC analysis showed that asparagine synthetase positive expression in dysplastic surgical margins and its higher expression was correlated with tumor recurrence and local relapse-free survival. Interpretations: We developed a panel of metabolic molecular markers to supplement the evaluation of negative and dysplastic margins. Fund: This study was supported by Nanjing Municipal Key Medical Laboratory Constructional Project Funding (Since 2012); Center of Nanjing Clinical Medicine Tumor (Since 2014). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Keywords: Oral squamous cell carcinoma, Amino acids metabolomics, Surgical margins, Asparagine synthetasehttp://www.sciencedirect.com/science/article/pii/S2352396419306693 |