Induced pluripotent stem cell line MUSIi006-A derived from hair follicle keratinocytes as a non-invasive somatic cell source

Induced pluripotent stem cell line MUSIi006-A derived from hair follicle keratinocytes as a non-invasive somatic cell source

In this study, we used hair follicle keratinocytes for reprogramming. Collection of plucked hairs offers advantages over other somatic cells because no medical professional or operation room is required. Keratinocytes were isolated from plucked hairs of a 21-year-old healthy woman and characterized...

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Main Authors: Bootsakorn Boonkaew, Lunnathaya Tapeng, Ratchapong Netsrithong, Chinnavuth Vatanashevanopakorn, Kovit Pattanapanyasat, Methichit Wattanapanitch
Format: Article
Language:English
Published: Elsevier 2018-08-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506118301752
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spelling doaj-c6759ee5bc194cc79c1469d9e01edb702020-11-24T22:26:33ZengElsevierStem Cell Research1873-50612018-08-01317982Induced pluripotent stem cell line MUSIi006-A derived from hair follicle keratinocytes as a non-invasive somatic cell sourceBootsakorn Boonkaew0Lunnathaya Tapeng1Ratchapong Netsrithong2Chinnavuth Vatanashevanopakorn3Kovit Pattanapanyasat4Methichit Wattanapanitch5Siriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandDepartment of Biology, Faculty of Science, Silpakorn University, Nakornpathom, ThailandSiriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Siriraj Center of Research Excellence for Microparticle and Exosome in Diseases, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, ThailandSiriraj Center for Regenerative Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Corresponding author.In this study, we used hair follicle keratinocytes for reprogramming. Collection of plucked hairs offers advantages over other somatic cells because no medical professional or operation room is required. Keratinocytes were isolated from plucked hairs of a 21-year-old healthy woman and characterized for the expression of cytokeratin 14 (CK14). Reprogramming of keratinocytes was performed using Sendai virus. Further characterization of the keratinocyte-derived iPSC line (designated as MUSIi006-A) confirmed that the cell line was pluripotent, free from Sendai viral genome and transgenes, and retained normal karyotype. Our method represents an easy, non-invasive and efficient approach for iPSC generation from hair samples.http://www.sciencedirect.com/science/article/pii/S1873506118301752
collection DOAJ
language English
format Article
sources DOAJ
author Bootsakorn Boonkaew
Lunnathaya Tapeng
Ratchapong Netsrithong
Chinnavuth Vatanashevanopakorn
Kovit Pattanapanyasat
Methichit Wattanapanitch
spellingShingle Bootsakorn Boonkaew
Lunnathaya Tapeng
Ratchapong Netsrithong
Chinnavuth Vatanashevanopakorn
Kovit Pattanapanyasat
Methichit Wattanapanitch
Induced pluripotent stem cell line MUSIi006-A derived from hair follicle keratinocytes as a non-invasive somatic cell source
Stem Cell Research
author_facet Bootsakorn Boonkaew
Lunnathaya Tapeng
Ratchapong Netsrithong
Chinnavuth Vatanashevanopakorn
Kovit Pattanapanyasat
Methichit Wattanapanitch
author_sort Bootsakorn Boonkaew
title Induced pluripotent stem cell line MUSIi006-A derived from hair follicle keratinocytes as a non-invasive somatic cell source
title_short Induced pluripotent stem cell line MUSIi006-A derived from hair follicle keratinocytes as a non-invasive somatic cell source
title_full Induced pluripotent stem cell line MUSIi006-A derived from hair follicle keratinocytes as a non-invasive somatic cell source
title_fullStr Induced pluripotent stem cell line MUSIi006-A derived from hair follicle keratinocytes as a non-invasive somatic cell source
title_full_unstemmed Induced pluripotent stem cell line MUSIi006-A derived from hair follicle keratinocytes as a non-invasive somatic cell source
title_sort induced pluripotent stem cell line musii006-a derived from hair follicle keratinocytes as a non-invasive somatic cell source
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2018-08-01
description In this study, we used hair follicle keratinocytes for reprogramming. Collection of plucked hairs offers advantages over other somatic cells because no medical professional or operation room is required. Keratinocytes were isolated from plucked hairs of a 21-year-old healthy woman and characterized for the expression of cytokeratin 14 (CK14). Reprogramming of keratinocytes was performed using Sendai virus. Further characterization of the keratinocyte-derived iPSC line (designated as MUSIi006-A) confirmed that the cell line was pluripotent, free from Sendai viral genome and transgenes, and retained normal karyotype. Our method represents an easy, non-invasive and efficient approach for iPSC generation from hair samples.
url http://www.sciencedirect.com/science/article/pii/S1873506118301752
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