Specificity in endoplasmic reticulum-stress signaling in yeast entails a step-wise engagement of HAC1 mRNA to clusters of the stress sensor Ire1
Insufficient protein-folding capacity in the endoplasmic reticulum (ER) induces the unfolded protein response (UPR). In the ER lumen, accumulation of unfolded proteins activates the transmembrane ER-stress sensor Ire1 and drives its oligomerization. In the cytosol, Ire1 recruits HAC1 mRNA, mediating...
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doaj-c6817a80e37e4180bc5d1af037ef86b92021-05-04T23:36:11ZengeLife Sciences Publications LtdeLife2050-084X2014-12-01310.7554/eLife.05031Specificity in endoplasmic reticulum-stress signaling in yeast entails a step-wise engagement of HAC1 mRNA to clusters of the stress sensor Ire1Eelco van Anken0David Pincus1Scott Coyle2Tomás Aragón3Christof Osman4Federica Lari5Silvia Gómez Puerta6Alexei V Korennykh7Peter Walter8Division of Genetics and Cell Biology, San Raffaele Scientific Institute, Milan, Italy; Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, United StatesDepartment of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, United StatesDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United StatesDepartment of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, United States; Department of Gene Therapy and Gene Regulation, Center for Applied Medical Research, Pamplona, SpainDepartment of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, United StatesDivision of Genetics and Cell Biology, San Raffaele Scientific Institute, Milan, ItalyDepartment of Gene Therapy and Gene Regulation, Center for Applied Medical Research, Pamplona, SpainDepartment of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, United StatesDepartment of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, United StatesInsufficient protein-folding capacity in the endoplasmic reticulum (ER) induces the unfolded protein response (UPR). In the ER lumen, accumulation of unfolded proteins activates the transmembrane ER-stress sensor Ire1 and drives its oligomerization. In the cytosol, Ire1 recruits HAC1 mRNA, mediating its non-conventional splicing. The spliced mRNA is translated into Hac1, the key transcription activator of UPR target genes that mitigate ER-stress. In this study, we report that oligomeric assembly of the ER-lumenal domain is sufficient to drive Ire1 clustering. Clustering facilitates Ire1's cytosolic oligomeric assembly and HAC1 mRNA docking onto a positively charged motif in Ire1's cytosolic linker domain that tethers the kinase/RNase to the transmembrane domain. By the use of a synthetic bypass, we demonstrate that mRNA docking per se is a pre-requisite for initiating Ire1's RNase activity and, hence, splicing. We posit that such step-wise engagement between Ire1 and its mRNA substrate contributes to selectivity and efficiency in UPR signaling.https://elifesciences.org/articles/05031stress signalingendoplasmic reticulumunfolded protein responsemRNA targetingmRNA processing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eelco van Anken David Pincus Scott Coyle Tomás Aragón Christof Osman Federica Lari Silvia Gómez Puerta Alexei V Korennykh Peter Walter |
spellingShingle |
Eelco van Anken David Pincus Scott Coyle Tomás Aragón Christof Osman Federica Lari Silvia Gómez Puerta Alexei V Korennykh Peter Walter Specificity in endoplasmic reticulum-stress signaling in yeast entails a step-wise engagement of HAC1 mRNA to clusters of the stress sensor Ire1 eLife stress signaling endoplasmic reticulum unfolded protein response mRNA targeting mRNA processing |
author_facet |
Eelco van Anken David Pincus Scott Coyle Tomás Aragón Christof Osman Federica Lari Silvia Gómez Puerta Alexei V Korennykh Peter Walter |
author_sort |
Eelco van Anken |
title |
Specificity in endoplasmic reticulum-stress signaling in yeast entails a step-wise engagement of HAC1 mRNA to clusters of the stress sensor Ire1 |
title_short |
Specificity in endoplasmic reticulum-stress signaling in yeast entails a step-wise engagement of HAC1 mRNA to clusters of the stress sensor Ire1 |
title_full |
Specificity in endoplasmic reticulum-stress signaling in yeast entails a step-wise engagement of HAC1 mRNA to clusters of the stress sensor Ire1 |
title_fullStr |
Specificity in endoplasmic reticulum-stress signaling in yeast entails a step-wise engagement of HAC1 mRNA to clusters of the stress sensor Ire1 |
title_full_unstemmed |
Specificity in endoplasmic reticulum-stress signaling in yeast entails a step-wise engagement of HAC1 mRNA to clusters of the stress sensor Ire1 |
title_sort |
specificity in endoplasmic reticulum-stress signaling in yeast entails a step-wise engagement of hac1 mrna to clusters of the stress sensor ire1 |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2014-12-01 |
description |
Insufficient protein-folding capacity in the endoplasmic reticulum (ER) induces the unfolded protein response (UPR). In the ER lumen, accumulation of unfolded proteins activates the transmembrane ER-stress sensor Ire1 and drives its oligomerization. In the cytosol, Ire1 recruits HAC1 mRNA, mediating its non-conventional splicing. The spliced mRNA is translated into Hac1, the key transcription activator of UPR target genes that mitigate ER-stress. In this study, we report that oligomeric assembly of the ER-lumenal domain is sufficient to drive Ire1 clustering. Clustering facilitates Ire1's cytosolic oligomeric assembly and HAC1 mRNA docking onto a positively charged motif in Ire1's cytosolic linker domain that tethers the kinase/RNase to the transmembrane domain. By the use of a synthetic bypass, we demonstrate that mRNA docking per se is a pre-requisite for initiating Ire1's RNase activity and, hence, splicing. We posit that such step-wise engagement between Ire1 and its mRNA substrate contributes to selectivity and efficiency in UPR signaling. |
topic |
stress signaling endoplasmic reticulum unfolded protein response mRNA targeting mRNA processing |
url |
https://elifesciences.org/articles/05031 |
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