Apparent size and morphology of bacterial microcompartments varies with technique.
Bacterial microcompartments (MCPs) are protein-based organelles that encapsulate metabolic pathways. Metabolic engineers have recently sought to repurpose MCPs to encapsulate heterologous pathways to increase flux through pathways of interest. As MCP engineering becomes more common, standardized met...
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doaj-c68887ec6b5d42c7a55e027fd3ff4c132021-03-03T21:32:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01153e022639510.1371/journal.pone.0226395Apparent size and morphology of bacterial microcompartments varies with technique.Nolan W KennedyJasmine M HersheweTaylor M NicholsEric W RothCharlene D WilkeCarolyn E MillsMichael C JewettDanielle Tullman-ErcekBacterial microcompartments (MCPs) are protein-based organelles that encapsulate metabolic pathways. Metabolic engineers have recently sought to repurpose MCPs to encapsulate heterologous pathways to increase flux through pathways of interest. As MCP engineering becomes more common, standardized methods for analyzing changes to MCPs and interpreting results across studies will become increasingly important. In this study, we demonstrate that different imaging techniques yield variations in the apparent size of purified MCPs from Salmonella enterica serovar Typhimurium LT2, likely due to variations in sample preparation methods. We provide guidelines for preparing samples for MCP imaging and outline expected variations in apparent size and morphology between methods. With this report we aim to establish an aid for comparing results across studies.https://doi.org/10.1371/journal.pone.0226395 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nolan W Kennedy Jasmine M Hershewe Taylor M Nichols Eric W Roth Charlene D Wilke Carolyn E Mills Michael C Jewett Danielle Tullman-Ercek |
spellingShingle |
Nolan W Kennedy Jasmine M Hershewe Taylor M Nichols Eric W Roth Charlene D Wilke Carolyn E Mills Michael C Jewett Danielle Tullman-Ercek Apparent size and morphology of bacterial microcompartments varies with technique. PLoS ONE |
author_facet |
Nolan W Kennedy Jasmine M Hershewe Taylor M Nichols Eric W Roth Charlene D Wilke Carolyn E Mills Michael C Jewett Danielle Tullman-Ercek |
author_sort |
Nolan W Kennedy |
title |
Apparent size and morphology of bacterial microcompartments varies with technique. |
title_short |
Apparent size and morphology of bacterial microcompartments varies with technique. |
title_full |
Apparent size and morphology of bacterial microcompartments varies with technique. |
title_fullStr |
Apparent size and morphology of bacterial microcompartments varies with technique. |
title_full_unstemmed |
Apparent size and morphology of bacterial microcompartments varies with technique. |
title_sort |
apparent size and morphology of bacterial microcompartments varies with technique. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2020-01-01 |
description |
Bacterial microcompartments (MCPs) are protein-based organelles that encapsulate metabolic pathways. Metabolic engineers have recently sought to repurpose MCPs to encapsulate heterologous pathways to increase flux through pathways of interest. As MCP engineering becomes more common, standardized methods for analyzing changes to MCPs and interpreting results across studies will become increasingly important. In this study, we demonstrate that different imaging techniques yield variations in the apparent size of purified MCPs from Salmonella enterica serovar Typhimurium LT2, likely due to variations in sample preparation methods. We provide guidelines for preparing samples for MCP imaging and outline expected variations in apparent size and morphology between methods. With this report we aim to establish an aid for comparing results across studies. |
url |
https://doi.org/10.1371/journal.pone.0226395 |
work_keys_str_mv |
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