Human adult mesangiogenic progenitor cells reveal an early angiogenic potential, which is lost after mesengenic differentiation
Abstract Background Mesangiogenic progenitor cells (MPCs) have shown the ability to differentiate in-vitro toward mesenchymal stromal cells (MSCs) as well as angiogenic potential. MPCs have so far been described in detail as progenitors of the mesodermal lineage and appear to be of great significanc...
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doaj-c6a269e110884a74ac9bc726663c567a2020-11-24T21:03:47ZengBMCStem Cell Research & Therapy1757-65122017-05-018111610.1186/s13287-017-0562-xHuman adult mesangiogenic progenitor cells reveal an early angiogenic potential, which is lost after mesengenic differentiationMarina Montali0Francesca M. Panvini1Serena Barachini2Francesca Ronca3Vittoria Carnicelli4Stefano Mazzoni5Iacopo Petrini6Simone Pacini7Department of Clinical and Experimental Medicine, Hematology Division, University of PisaDepartment of Clinical and Experimental Medicine, Hematology Division, University of PisaDepartment of Clinical and Experimental Medicine, Hematology Division, University of PisaDepartment of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of PisaDepartment of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of PisaDepartment of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of PisaDepartment of Translational Research and New Technology in Medicine, University of PisaDepartment of Clinical and Experimental Medicine, Hematology Division, University of PisaAbstract Background Mesangiogenic progenitor cells (MPCs) have shown the ability to differentiate in-vitro toward mesenchymal stromal cells (MSCs) as well as angiogenic potential. MPCs have so far been described in detail as progenitors of the mesodermal lineage and appear to be of great significance in tissue regeneration and in hemopoietic niche regulation. On the contrary, information regarding the MPC angiogenic process is still incomplete and requires further clarification. In particular, genuine MPC angiogenic potential should be confirmed in-vivo. Methods In the present article, markers and functions associated with angiogenic cells have been dissected. MPCs freshly isolated from human bone marrow have been induced to differentiate into exponentially growing MSCs (P2-MSCs). Cells have been characterized and angiogenesis-related gene expression was evaluated before and after mesengenic differentiation. Moreover, angiogenic potential has been tested by in-vitro and in-vivo functional assays. Results MPCs showed a distinctive gene expression profile, acetylated-low density lipoprotein uptake, and transendothelial migration capacity. However, mature endothelial markers and functions of endothelial cells, including the ability to form new capillaries, were absent, thus suggesting MPCs to be very immature endothelial progenitors. MPCs showed marked 3D spheroid sprouting activating the related molecular machinery, a clear in-vitro indication of early angiogenesis. Indeed, MPCs applied to chicken chorioallantoic membrane induced and participated in neovessel formation. All of these features were lost in mesengenic terminally differentiated P2-MSCs, showing definite separation of the two differentiation lineages. Conclusion Our results confirm the bona-fide angiogenic potential of MPCs and suggest that the high variability reported for MSC cultures, responsible for the controversies regarding MSC angiogenic potential, could be correlated to variable percentages of co-isolated MPCs in the different culture conditions so far used.http://link.springer.com/article/10.1186/s13287-017-0562-xMesangiogenic progenitor cellsAngiogenesisMesenchymal stromal cellsNestinBone marrow-derived cellsSprouting |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marina Montali Francesca M. Panvini Serena Barachini Francesca Ronca Vittoria Carnicelli Stefano Mazzoni Iacopo Petrini Simone Pacini |
spellingShingle |
Marina Montali Francesca M. Panvini Serena Barachini Francesca Ronca Vittoria Carnicelli Stefano Mazzoni Iacopo Petrini Simone Pacini Human adult mesangiogenic progenitor cells reveal an early angiogenic potential, which is lost after mesengenic differentiation Stem Cell Research & Therapy Mesangiogenic progenitor cells Angiogenesis Mesenchymal stromal cells Nestin Bone marrow-derived cells Sprouting |
author_facet |
Marina Montali Francesca M. Panvini Serena Barachini Francesca Ronca Vittoria Carnicelli Stefano Mazzoni Iacopo Petrini Simone Pacini |
author_sort |
Marina Montali |
title |
Human adult mesangiogenic progenitor cells reveal an early angiogenic potential, which is lost after mesengenic differentiation |
title_short |
Human adult mesangiogenic progenitor cells reveal an early angiogenic potential, which is lost after mesengenic differentiation |
title_full |
Human adult mesangiogenic progenitor cells reveal an early angiogenic potential, which is lost after mesengenic differentiation |
title_fullStr |
Human adult mesangiogenic progenitor cells reveal an early angiogenic potential, which is lost after mesengenic differentiation |
title_full_unstemmed |
Human adult mesangiogenic progenitor cells reveal an early angiogenic potential, which is lost after mesengenic differentiation |
title_sort |
human adult mesangiogenic progenitor cells reveal an early angiogenic potential, which is lost after mesengenic differentiation |
publisher |
BMC |
series |
Stem Cell Research & Therapy |
issn |
1757-6512 |
publishDate |
2017-05-01 |
description |
Abstract Background Mesangiogenic progenitor cells (MPCs) have shown the ability to differentiate in-vitro toward mesenchymal stromal cells (MSCs) as well as angiogenic potential. MPCs have so far been described in detail as progenitors of the mesodermal lineage and appear to be of great significance in tissue regeneration and in hemopoietic niche regulation. On the contrary, information regarding the MPC angiogenic process is still incomplete and requires further clarification. In particular, genuine MPC angiogenic potential should be confirmed in-vivo. Methods In the present article, markers and functions associated with angiogenic cells have been dissected. MPCs freshly isolated from human bone marrow have been induced to differentiate into exponentially growing MSCs (P2-MSCs). Cells have been characterized and angiogenesis-related gene expression was evaluated before and after mesengenic differentiation. Moreover, angiogenic potential has been tested by in-vitro and in-vivo functional assays. Results MPCs showed a distinctive gene expression profile, acetylated-low density lipoprotein uptake, and transendothelial migration capacity. However, mature endothelial markers and functions of endothelial cells, including the ability to form new capillaries, were absent, thus suggesting MPCs to be very immature endothelial progenitors. MPCs showed marked 3D spheroid sprouting activating the related molecular machinery, a clear in-vitro indication of early angiogenesis. Indeed, MPCs applied to chicken chorioallantoic membrane induced and participated in neovessel formation. All of these features were lost in mesengenic terminally differentiated P2-MSCs, showing definite separation of the two differentiation lineages. Conclusion Our results confirm the bona-fide angiogenic potential of MPCs and suggest that the high variability reported for MSC cultures, responsible for the controversies regarding MSC angiogenic potential, could be correlated to variable percentages of co-isolated MPCs in the different culture conditions so far used. |
topic |
Mesangiogenic progenitor cells Angiogenesis Mesenchymal stromal cells Nestin Bone marrow-derived cells Sprouting |
url |
http://link.springer.com/article/10.1186/s13287-017-0562-x |
work_keys_str_mv |
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