Expression of calcitonin gene-related peptide and pulp sensitivity tests in irreversible pulpitis
Abstract The aim of the present study was to identify the relationship between the expression of calcitonin gene-related peptide (CGRP) and the responses of pulp sensitivity tests in healthy pulps and irreversible pulps by performing a cross-sectional study on patients. Two hundred subjects were eva...
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doaj-c6c539cbf6dc45f6a4faa8d43b8f4efc2020-11-25T00:48:18ZengSociedade Brasileira de Pesquisa Odontológica Brazilian Oral Research1807-31073310.1590/1807-3107bor-2019.vol33.0077S1806-83242019000100251Expression of calcitonin gene-related peptide and pulp sensitivity tests in irreversible pulpitisBrenda Eréndida CASTILLO-SILVAVerónica MARTÍNEZ-JIMÉNEZGabriel Alejandro MARTÍNEZ- CASTAÑÓNCarlo Eduardo MEDINA-SOLÍSEva Concepción AGUIRRE-LÓPEZJesús Ramón CASTILLO-HERNÁNDEZNereyda NIÑO-MARTÍNEZNuria PATIÑO-MARÍNAbstract The aim of the present study was to identify the relationship between the expression of calcitonin gene-related peptide (CGRP) and the responses of pulp sensitivity tests in healthy pulps and irreversible pulps by performing a cross-sectional study on patients. Two hundred subjects were evaluated. A total of 75 subjects complied with the criteria. The participants were divided into two groups: a) Healthy pulp (subjects [n = 35] having posterior teeth with clinically normal pulp tissue), and b) Irreversible pulpitis (subjects [n = 40] having posterior teeth with irreversible pulpitis). All participants were evaluated using the following variables: a) medical and dental history, b) pulp sensitivity tests, c) expression of CGRP by the enzyme-linked immunosorbent assay (ELISA), and d) expression levels of mRNA CGRP and mRNA CGRP receptor genes. We determined that the responses of the cold test between 4 and ≥12 s presented a higher average of the expression of CGRP in the group having irreversible pulpitis (p = 0.0001). When we compared the groups with the value of the electrical impulse, we found statistically significant differences (p = 0.0001), observing positive responses to the test with electrical impulses of 7 to 10, with an average of 72.15 ng/mL of CGRP in the irreversible pulpitis group. High values of CGRP expression were observed in that group in the responses of pulp sensitivity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100251&lng=en&tlng=enCalcitonin Gene-Related PeptideDental pulp test |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Brenda Eréndida CASTILLO-SILVA Verónica MARTÍNEZ-JIMÉNEZ Gabriel Alejandro MARTÍNEZ- CASTAÑÓN Carlo Eduardo MEDINA-SOLÍS Eva Concepción AGUIRRE-LÓPEZ Jesús Ramón CASTILLO-HERNÁNDEZ Nereyda NIÑO-MARTÍNEZ Nuria PATIÑO-MARÍN |
spellingShingle |
Brenda Eréndida CASTILLO-SILVA Verónica MARTÍNEZ-JIMÉNEZ Gabriel Alejandro MARTÍNEZ- CASTAÑÓN Carlo Eduardo MEDINA-SOLÍS Eva Concepción AGUIRRE-LÓPEZ Jesús Ramón CASTILLO-HERNÁNDEZ Nereyda NIÑO-MARTÍNEZ Nuria PATIÑO-MARÍN Expression of calcitonin gene-related peptide and pulp sensitivity tests in irreversible pulpitis Brazilian Oral Research Calcitonin Gene-Related Peptide Dental pulp test |
author_facet |
Brenda Eréndida CASTILLO-SILVA Verónica MARTÍNEZ-JIMÉNEZ Gabriel Alejandro MARTÍNEZ- CASTAÑÓN Carlo Eduardo MEDINA-SOLÍS Eva Concepción AGUIRRE-LÓPEZ Jesús Ramón CASTILLO-HERNÁNDEZ Nereyda NIÑO-MARTÍNEZ Nuria PATIÑO-MARÍN |
author_sort |
Brenda Eréndida CASTILLO-SILVA |
title |
Expression of calcitonin gene-related peptide and pulp sensitivity tests in irreversible pulpitis |
title_short |
Expression of calcitonin gene-related peptide and pulp sensitivity tests in irreversible pulpitis |
title_full |
Expression of calcitonin gene-related peptide and pulp sensitivity tests in irreversible pulpitis |
title_fullStr |
Expression of calcitonin gene-related peptide and pulp sensitivity tests in irreversible pulpitis |
title_full_unstemmed |
Expression of calcitonin gene-related peptide and pulp sensitivity tests in irreversible pulpitis |
title_sort |
expression of calcitonin gene-related peptide and pulp sensitivity tests in irreversible pulpitis |
publisher |
Sociedade Brasileira de Pesquisa Odontológica |
series |
Brazilian Oral Research |
issn |
1807-3107 |
description |
Abstract The aim of the present study was to identify the relationship between the expression of calcitonin gene-related peptide (CGRP) and the responses of pulp sensitivity tests in healthy pulps and irreversible pulps by performing a cross-sectional study on patients. Two hundred subjects were evaluated. A total of 75 subjects complied with the criteria. The participants were divided into two groups: a) Healthy pulp (subjects [n = 35] having posterior teeth with clinically normal pulp tissue), and b) Irreversible pulpitis (subjects [n = 40] having posterior teeth with irreversible pulpitis). All participants were evaluated using the following variables: a) medical and dental history, b) pulp sensitivity tests, c) expression of CGRP by the enzyme-linked immunosorbent assay (ELISA), and d) expression levels of mRNA CGRP and mRNA CGRP receptor genes. We determined that the responses of the cold test between 4 and ≥12 s presented a higher average of the expression of CGRP in the group having irreversible pulpitis (p = 0.0001). When we compared the groups with the value of the electrical impulse, we found statistically significant differences (p = 0.0001), observing positive responses to the test with electrical impulses of 7 to 10, with an average of 72.15 ng/mL of CGRP in the irreversible pulpitis group. High values of CGRP expression were observed in that group in the responses of pulp sensitivity. |
topic |
Calcitonin Gene-Related Peptide Dental pulp test |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100251&lng=en&tlng=en |
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