Expression of calcitonin gene-related peptide and pulp sensitivity tests in irreversible pulpitis

• Main Authors: Brenda Eréndida CASTILLO-SILVA, Verónica MARTÍNEZ-JIMÉNEZ, Gabriel Alejandro MARTÍNEZ- CASTAÑÓN, Carlo Eduardo MEDINA-SOLÍS, Eva Concepción AGUIRRE-LÓPEZ, Jesús Ramón CASTILLO-HERNÁNDEZ, Nereyda NIÑO-MARTÍNEZ, Nuria PATIÑO-MARÍN
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Pesquisa Odontológica
• Series: Brazilian Oral Research
• Subjects: Calcitonin Gene-Related Peptide; Dental pulp test
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242019000100251&lng=en&tlng=en

Abstract The aim of the present study was to identify the relationship between the expression of calcitonin gene-related peptide (CGRP) and the responses of pulp sensitivity tests in healthy pulps and irreversible pulps by performing a cross-sectional study on patients. Two hundred subjects were evaluated. A total of 75 subjects complied with the criteria. The participants were divided into two groups: a) Healthy pulp (subjects [n = 35] having posterior teeth with clinically normal pulp tissue), and b) Irreversible pulpitis (subjects [n = 40] having posterior teeth with irreversible pulpitis). All participants were evaluated using the following variables: a) medical and dental history, b) pulp sensitivity tests, c) expression of CGRP by the enzyme-linked immunosorbent assay (ELISA), and d) expression levels of mRNA CGRP and mRNA CGRP receptor genes. We determined that the responses of the cold test between 4 and ≥12 s presented a higher average of the expression of CGRP in the group having irreversible pulpitis (p = 0.0001). When we compared the groups with the value of the electrical impulse, we found statistically significant differences (p = 0.0001), observing positive responses to the test with electrical impulses of 7 to 10, with an average of 72.15 ng/mL of CGRP in the irreversible pulpitis group. High values of CGRP expression were observed in that group in the responses of pulp sensitivity.