The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae
In Saccharomyces cerevisiae and in humans, the telomerase RNA subunit is bound by Ku, a ring-shaped protein heterodimer best known for its function in DNA repair. Ku binding to yeast telomerase RNA promotes telomere lengthening and telomerase recruitment to telomeres, but how this is achieved remain...
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2015-07-01
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doaj-c6c74c4086534652942ff3ea46d6c5992021-05-04T23:56:14ZengeLife Sciences Publications LtdeLife2050-084X2015-07-01410.7554/eLife.07750The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiaeEvan P Hass0David C Zappulla1https://orcid.org/0000-0001-8242-3493Department of Biology, Johns Hopkins University, Baltimore, United StatesDepartment of Biology, Johns Hopkins University, Baltimore, United StatesIn Saccharomyces cerevisiae and in humans, the telomerase RNA subunit is bound by Ku, a ring-shaped protein heterodimer best known for its function in DNA repair. Ku binding to yeast telomerase RNA promotes telomere lengthening and telomerase recruitment to telomeres, but how this is achieved remains unknown. Using telomere-length analysis and chromatin immunoprecipitation, we show that Sir4 – a previously identified Ku-binding protein that is a component of telomeric silent chromatin – is required for Ku-mediated telomere lengthening and telomerase recruitment. We also find that specifically tethering Sir4 directly to Ku-binding-defective telomerase RNA restores otherwise-shortened telomeres to wild-type length. These findings suggest that Sir4 is the telomere-bound target of Ku-mediated telomerase recruitment and provide one mechanism for how the Sir4-competing Rif1 and Rif2 proteins negatively regulate telomere length in yeast.https://elifesciences.org/articles/07750telomerasetelomereyeastS. cerevisiaetranscriptional silencingKu |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Evan P Hass David C Zappulla |
spellingShingle |
Evan P Hass David C Zappulla The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae eLife telomerase telomere yeast S. cerevisiae transcriptional silencing Ku |
author_facet |
Evan P Hass David C Zappulla |
author_sort |
Evan P Hass |
title |
The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae |
title_short |
The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae |
title_full |
The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae |
title_fullStr |
The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae |
title_full_unstemmed |
The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae |
title_sort |
ku subunit of telomerase binds sir4 to recruit telomerase to lengthen telomeres in s. cerevisiae |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2015-07-01 |
description |
In Saccharomyces cerevisiae and in humans, the telomerase RNA subunit is bound by Ku, a ring-shaped protein heterodimer best known for its function in DNA repair. Ku binding to yeast telomerase RNA promotes telomere lengthening and telomerase recruitment to telomeres, but how this is achieved remains unknown. Using telomere-length analysis and chromatin immunoprecipitation, we show that Sir4 – a previously identified Ku-binding protein that is a component of telomeric silent chromatin – is required for Ku-mediated telomere lengthening and telomerase recruitment. We also find that specifically tethering Sir4 directly to Ku-binding-defective telomerase RNA restores otherwise-shortened telomeres to wild-type length. These findings suggest that Sir4 is the telomere-bound target of Ku-mediated telomerase recruitment and provide one mechanism for how the Sir4-competing Rif1 and Rif2 proteins negatively regulate telomere length in yeast. |
topic |
telomerase telomere yeast S. cerevisiae transcriptional silencing Ku |
url |
https://elifesciences.org/articles/07750 |
work_keys_str_mv |
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1721476777390374912 |