Acupuncture Treatment Reverses Retinal Gene Expression Induced by Optic Nerve Injury via RNA Sequencing Analysis
Glaucoma and traumatic optic nerve crush (ONC) injury result in progressive loss of retinal ganglion cells (RGCs) and defects in visual function. In clinical trials of Traditional Chinese Medicine, acupuncture has been widely used for the treatment of ocular diseases. However, the molecular mechanis...
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2019-10-01
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doaj-c6df5a2f0c4348c68c1cc5dc7c5870722020-11-25T00:04:11ZengFrontiers Media S.A.Frontiers in Integrative Neuroscience1662-51452019-10-011310.3389/fnint.2019.00059470837Acupuncture Treatment Reverses Retinal Gene Expression Induced by Optic Nerve Injury via RNA Sequencing AnalysisJie Chen0Li Zhang1Lanying Liu2Xueqin Yang3Fengzhi Wu4Xiulun Gan5Rong Zhang6Yinjia He7Qiuyi Lv8Haonan Fu9Ling Zhou10Jiaxi Zhang11Anming Liu12Xiaodong Liu13Linqing Miao14School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaThe Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, ChinaJournal Center, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, ChinaBeijing Advanced Innovation Center for Intelligent Robots and Systems, Beijing Institute of Technology, Beijing, ChinaGlaucoma and traumatic optic nerve crush (ONC) injury result in progressive loss of retinal ganglion cells (RGCs) and defects in visual function. In clinical trials of Traditional Chinese Medicine, acupuncture has been widely used for the treatment of ocular diseases. However, the molecular mechanisms of acupuncture treatment are still unclear. In this study, we used technique of RNA sequencing (RNA-seq) to study the effects of acupuncture treatment on retinal transcriptome after axotomy injury. RNA-seq results revealed that 436 genes including 31 transcription factors (TFs) were changed after injury, among them were many well-known neural degeneration related TFs such as Jun, Ddit3, Atf3, and Atf4. Interestingly, acupuncture treatment at acupoint GB20 (Fengchi) significantly reversed a series of differential expressed genes (DEGs) induced by optic nerve injury. While treatments at BL1 (Jingming) or GB20 sham control acupoint-GV16 (Fengfu), led to limited DEG reversal. In contrast, treatments at these two sites further enhanced the trend of DEG expression induced by axotomy injury. At last, retina immunostaining results revealed that only GB20 acupoint treatment increased RGC survival, in consistent with RNA-seq results. Therefore, our study first reported that acupuncture treatment regulated retinal transcriptome and reversed the gene expression induced by axotomy injury, and GB20 acupoint treatment increased RGC survival, which will provide novel therapeutic targets for treatment of ocular diseases.https://www.frontiersin.org/article/10.3389/fnint.2019.00059/fulloptic nerve crushretinal ganglion cellsacupunctureRNA sequencingtranscriptomeGB20 (Fengchi) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jie Chen Li Zhang Lanying Liu Xueqin Yang Fengzhi Wu Xiulun Gan Rong Zhang Yinjia He Qiuyi Lv Haonan Fu Ling Zhou Jiaxi Zhang Anming Liu Xiaodong Liu Linqing Miao |
spellingShingle |
Jie Chen Li Zhang Lanying Liu Xueqin Yang Fengzhi Wu Xiulun Gan Rong Zhang Yinjia He Qiuyi Lv Haonan Fu Ling Zhou Jiaxi Zhang Anming Liu Xiaodong Liu Linqing Miao Acupuncture Treatment Reverses Retinal Gene Expression Induced by Optic Nerve Injury via RNA Sequencing Analysis Frontiers in Integrative Neuroscience optic nerve crush retinal ganglion cells acupuncture RNA sequencing transcriptome GB20 (Fengchi) |
author_facet |
Jie Chen Li Zhang Lanying Liu Xueqin Yang Fengzhi Wu Xiulun Gan Rong Zhang Yinjia He Qiuyi Lv Haonan Fu Ling Zhou Jiaxi Zhang Anming Liu Xiaodong Liu Linqing Miao |
author_sort |
Jie Chen |
title |
Acupuncture Treatment Reverses Retinal Gene Expression Induced by Optic Nerve Injury via RNA Sequencing Analysis |
title_short |
Acupuncture Treatment Reverses Retinal Gene Expression Induced by Optic Nerve Injury via RNA Sequencing Analysis |
title_full |
Acupuncture Treatment Reverses Retinal Gene Expression Induced by Optic Nerve Injury via RNA Sequencing Analysis |
title_fullStr |
Acupuncture Treatment Reverses Retinal Gene Expression Induced by Optic Nerve Injury via RNA Sequencing Analysis |
title_full_unstemmed |
Acupuncture Treatment Reverses Retinal Gene Expression Induced by Optic Nerve Injury via RNA Sequencing Analysis |
title_sort |
acupuncture treatment reverses retinal gene expression induced by optic nerve injury via rna sequencing analysis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Integrative Neuroscience |
issn |
1662-5145 |
publishDate |
2019-10-01 |
description |
Glaucoma and traumatic optic nerve crush (ONC) injury result in progressive loss of retinal ganglion cells (RGCs) and defects in visual function. In clinical trials of Traditional Chinese Medicine, acupuncture has been widely used for the treatment of ocular diseases. However, the molecular mechanisms of acupuncture treatment are still unclear. In this study, we used technique of RNA sequencing (RNA-seq) to study the effects of acupuncture treatment on retinal transcriptome after axotomy injury. RNA-seq results revealed that 436 genes including 31 transcription factors (TFs) were changed after injury, among them were many well-known neural degeneration related TFs such as Jun, Ddit3, Atf3, and Atf4. Interestingly, acupuncture treatment at acupoint GB20 (Fengchi) significantly reversed a series of differential expressed genes (DEGs) induced by optic nerve injury. While treatments at BL1 (Jingming) or GB20 sham control acupoint-GV16 (Fengfu), led to limited DEG reversal. In contrast, treatments at these two sites further enhanced the trend of DEG expression induced by axotomy injury. At last, retina immunostaining results revealed that only GB20 acupoint treatment increased RGC survival, in consistent with RNA-seq results. Therefore, our study first reported that acupuncture treatment regulated retinal transcriptome and reversed the gene expression induced by axotomy injury, and GB20 acupoint treatment increased RGC survival, which will provide novel therapeutic targets for treatment of ocular diseases. |
topic |
optic nerve crush retinal ganglion cells acupuncture RNA sequencing transcriptome GB20 (Fengchi) |
url |
https://www.frontiersin.org/article/10.3389/fnint.2019.00059/full |
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