NCR3 polymorphism, haematological parameters, and severe malaria in Senegalese patients
Background Host factors, including host genetic variation, have been shown to influence the outcome of Plasmodium falciparum infection. Genome-wide linkage studies have mapped mild malaria resistance genes on chromosome 6p21, whereas NCR3-412 polymorphism (rs2736191) lying within this region was fou...
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doaj-c6e14d25db7d43359fec97131f45b7ff2020-11-24T20:54:41ZengPeerJ Inc.PeerJ2167-83592018-12-016e604810.7717/peerj.6048NCR3 polymorphism, haematological parameters, and severe malaria in Senegalese patientsAlassane Thiam0Sabrina Baaklini1Babacar Mbengue2Samia Nisar3Maryam Diarra4Sandrine Marquet5Mouhamadou Mansour Fall6Michel Sanka7Fatou Thiam8Rokhaya Ndiaye Diallo9Magali Torres10Alioune Dieye11Pascal Rihet12Unité d’Immunogénétique, Institut Pasteur de Dakar, Dakar, SenegalAix Marseille Univ, INSERM, TAGC, Marseille, FranceService d’Immunologie, University Cheikh Anta Diop of Dakar, Dakar, SenegalAix Marseille Univ, INSERM, TAGC, Marseille, FranceG4 Biostatistique, Institut Pasteur de Dakar, Dakar, SénégalAix Marseille Univ, INSERM, TAGC, Marseille, FranceService de Réanimation, Hôpital Principal de Dakar, Dakar, SenegalAix Marseille Univ, INSERM, TAGC, Marseille, FranceUnité d’Immunogénétique, Institut Pasteur de Dakar, Dakar, SenegalUnité d’Immunogénétique, Institut Pasteur de Dakar, Dakar, SenegalAix Marseille Univ, INSERM, TAGC, Marseille, FranceUnité d’Immunogénétique, Institut Pasteur de Dakar, Dakar, SenegalAix Marseille Univ, INSERM, TAGC, Marseille, FranceBackground Host factors, including host genetic variation, have been shown to influence the outcome of Plasmodium falciparum infection. Genome-wide linkage studies have mapped mild malaria resistance genes on chromosome 6p21, whereas NCR3-412 polymorphism (rs2736191) lying within this region was found to be associated with mild malaria. Methods Blood samples were taken from 188 Plasmodium falciparum malaria patients (76 mild malaria patients, 85 cerebral malaria patients, and 27 severe non-cerebral malaria patients). NCR3-412 (rs2736191) was analysed by sequencing, and haematological parameters were measured. Finally, their association with clinical phenotypes was assessed. Results We evidenced an association of thrombocytopenia with both cerebral malaria and severe non-cerebral malaria, and of an association of high leukocyte count with cerebral malaria. Additionally, we found no association of NCR3-412 with either cerebral malaria, severe non-cerebral malaria, or severe malaria after grouping cerebral malaria and severe non-cerebral malaria patients. Conclusions Our results suggest that NCR3 genetic variation has no effect, or only a small effect on the occurrence of severe malaria, although it has been strongly associated with mild malaria. We discuss the biological meaning of these results. Besides, we confirmed the association of thrombocytopenia and high leukocyte count with severe malaria phenotypes.https://peerj.com/articles/6048.pdfGenetic associationMild malariaSevere malariaPlasmodium falciparumHost factors |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alassane Thiam Sabrina Baaklini Babacar Mbengue Samia Nisar Maryam Diarra Sandrine Marquet Mouhamadou Mansour Fall Michel Sanka Fatou Thiam Rokhaya Ndiaye Diallo Magali Torres Alioune Dieye Pascal Rihet |
spellingShingle |
Alassane Thiam Sabrina Baaklini Babacar Mbengue Samia Nisar Maryam Diarra Sandrine Marquet Mouhamadou Mansour Fall Michel Sanka Fatou Thiam Rokhaya Ndiaye Diallo Magali Torres Alioune Dieye Pascal Rihet NCR3 polymorphism, haematological parameters, and severe malaria in Senegalese patients PeerJ Genetic association Mild malaria Severe malaria Plasmodium falciparum Host factors |
author_facet |
Alassane Thiam Sabrina Baaklini Babacar Mbengue Samia Nisar Maryam Diarra Sandrine Marquet Mouhamadou Mansour Fall Michel Sanka Fatou Thiam Rokhaya Ndiaye Diallo Magali Torres Alioune Dieye Pascal Rihet |
author_sort |
Alassane Thiam |
title |
NCR3 polymorphism, haematological parameters, and severe malaria in Senegalese patients |
title_short |
NCR3 polymorphism, haematological parameters, and severe malaria in Senegalese patients |
title_full |
NCR3 polymorphism, haematological parameters, and severe malaria in Senegalese patients |
title_fullStr |
NCR3 polymorphism, haematological parameters, and severe malaria in Senegalese patients |
title_full_unstemmed |
NCR3 polymorphism, haematological parameters, and severe malaria in Senegalese patients |
title_sort |
ncr3 polymorphism, haematological parameters, and severe malaria in senegalese patients |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2018-12-01 |
description |
Background Host factors, including host genetic variation, have been shown to influence the outcome of Plasmodium falciparum infection. Genome-wide linkage studies have mapped mild malaria resistance genes on chromosome 6p21, whereas NCR3-412 polymorphism (rs2736191) lying within this region was found to be associated with mild malaria. Methods Blood samples were taken from 188 Plasmodium falciparum malaria patients (76 mild malaria patients, 85 cerebral malaria patients, and 27 severe non-cerebral malaria patients). NCR3-412 (rs2736191) was analysed by sequencing, and haematological parameters were measured. Finally, their association with clinical phenotypes was assessed. Results We evidenced an association of thrombocytopenia with both cerebral malaria and severe non-cerebral malaria, and of an association of high leukocyte count with cerebral malaria. Additionally, we found no association of NCR3-412 with either cerebral malaria, severe non-cerebral malaria, or severe malaria after grouping cerebral malaria and severe non-cerebral malaria patients. Conclusions Our results suggest that NCR3 genetic variation has no effect, or only a small effect on the occurrence of severe malaria, although it has been strongly associated with mild malaria. We discuss the biological meaning of these results. Besides, we confirmed the association of thrombocytopenia and high leukocyte count with severe malaria phenotypes. |
topic |
Genetic association Mild malaria Severe malaria Plasmodium falciparum Host factors |
url |
https://peerj.com/articles/6048.pdf |
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