Diagnostic Utility of PAX5 in Hodgkin and Non-Hodgkin Lymphoma: A Study from Northern India
Introduction: PAX5 is an immunomarker of B-cell origin and useful in the diagnosis of lymphoma. There is hardly any study on PAX5 expression in Indian patients with lymphoma. Aim: To evaluate the diagnostic utility of PAX5 as an adjunct immunohistochemical marker in the diagnosis of Hodgkin Lym...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
JCDR Research and Publications Private Limited
2016-08-01
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Series: | Journal of Clinical and Diagnostic Research |
Subjects: | |
Online Access: | https://jcdr.net/articles/PDF/8352/21476_CE[Ra1]_F(GH)_PF1(ROAK)_PFA(AK)_PF2(EkGH).pdf |
Summary: | Introduction: PAX5 is an immunomarker of B-cell origin and
useful in the diagnosis of lymphoma. There is hardly any study
on PAX5 expression in Indian patients with lymphoma.
Aim: To evaluate the diagnostic utility of PAX5 as an adjunct
immunohistochemical marker in the diagnosis of Hodgkin
Lymphoma (HL) and Non-Hodgkin Lymphoma (NHL).
Materials and Methods: Immunohistochemistry was performed
against CD20, CD3, CD15, CD30, and PAX5 on formalin fixed
paraffin embedded tissue of 71 cases of lymphoma and CD20, CD3
and PAX5 in control samples of reactive lymph nodes. Frequency,
mean values, and percentage were calculated. Fisher’s-exact
test and test for analysis of variance were applied.
Result: For 24 cases of HL and 47 cases of NHL, the mean age of
patients was 17.6±14.8 years and 44.1±21.6 years, respectively.
The male: female ratio for both HL and NHL were 1.7:1. Among
NHL cases, the numbers of B-cell and T-cell types were 39/47
(83%) and 8/47 (17%), respectively. In comparison to control
samples, PAX5+ expression was seen in 23/24 (95.8%) cases
of HL (p=1.000) and 32/39 (82%) cases of B-NHL (p=0.0834).
All the cases of T-NHL showed negative expression of PAX5
(p<0.0001). Analysis of variance between NHL, HL and control
samples was statistically significant (p<0.0001).
Conclusion: PAX5 staining between control samples and cases
of classical HL and B-NHL was statistically not significant,
whereas, statistically significant difference was observed with
T-NHL. Thus, PAX5 may be used as an adjunct marker in the
diagnosis of classical HL and B-NHL. |
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ISSN: | 2249-782X 0973-709X |