High-fidelity DNA ligation enforces accurate Okazaki fragment maturation during DNA replication

DNA ligase 1 (LIG1) finalizes eukaryotic nuclear DNA synthesis by sealing Okazaki fragments using DNA end-joining reactions. Here the authors, by studying an engineered low-fidelity LIG1, reveal that LIG1 is a highly accurate DNA ligase in vivo.

Bibliographic Details
Main Authors:	Jessica S. Williams, Percy P. Tumbale, Mercedes E. Arana, Julian A. Rana, R. Scott Williams, Thomas A. Kunkel
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-01-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-20800-1

id	doaj-c7070beaf4664cbf8e117033397ef7e8
record_format	Article
spelling	doaj-c7070beaf4664cbf8e117033397ef7e82021-01-24T12:11:08ZengNature Publishing GroupNature Communications2041-17232021-01-0112111110.1038/s41467-020-20800-1High-fidelity DNA ligation enforces accurate Okazaki fragment maturation during DNA replicationJessica S. Williams0Percy P. Tumbale1Mercedes E. Arana2Julian A. Rana3R. Scott Williams4Thomas A. Kunkel5Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, US National Institutes of Health, Department of Health and Human ServicesGenome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, US National Institutes of Health, Department of Health and Human ServicesGenome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, US National Institutes of Health, Department of Health and Human ServicesGenome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, US National Institutes of Health, Department of Health and Human ServicesGenome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, US National Institutes of Health, Department of Health and Human ServicesGenome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, US National Institutes of Health, Department of Health and Human ServicesDNA ligase 1 (LIG1) finalizes eukaryotic nuclear DNA synthesis by sealing Okazaki fragments using DNA end-joining reactions. Here the authors, by studying an engineered low-fidelity LIG1, reveal that LIG1 is a highly accurate DNA ligase in vivo.https://doi.org/10.1038/s41467-020-20800-1
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jessica S. Williams Percy P. Tumbale Mercedes E. Arana Julian A. Rana R. Scott Williams Thomas A. Kunkel
spellingShingle	Jessica S. Williams Percy P. Tumbale Mercedes E. Arana Julian A. Rana R. Scott Williams Thomas A. Kunkel High-fidelity DNA ligation enforces accurate Okazaki fragment maturation during DNA replication Nature Communications
author_facet	Jessica S. Williams Percy P. Tumbale Mercedes E. Arana Julian A. Rana R. Scott Williams Thomas A. Kunkel
author_sort	Jessica S. Williams
title	High-fidelity DNA ligation enforces accurate Okazaki fragment maturation during DNA replication
title_short	High-fidelity DNA ligation enforces accurate Okazaki fragment maturation during DNA replication
title_full	High-fidelity DNA ligation enforces accurate Okazaki fragment maturation during DNA replication
title_fullStr	High-fidelity DNA ligation enforces accurate Okazaki fragment maturation during DNA replication
title_full_unstemmed	High-fidelity DNA ligation enforces accurate Okazaki fragment maturation during DNA replication
title_sort	high-fidelity dna ligation enforces accurate okazaki fragment maturation during dna replication
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2021-01-01
description	DNA ligase 1 (LIG1) finalizes eukaryotic nuclear DNA synthesis by sealing Okazaki fragments using DNA end-joining reactions. Here the authors, by studying an engineered low-fidelity LIG1, reveal that LIG1 is a highly accurate DNA ligase in vivo.
url	https://doi.org/10.1038/s41467-020-20800-1
work_keys_str_mv	AT jessicaswilliams highfidelitydnaligationenforcesaccurateokazakifragmentmaturationduringdnareplication AT percyptumbale highfidelitydnaligationenforcesaccurateokazakifragmentmaturationduringdnareplication AT mercedesearana highfidelitydnaligationenforcesaccurateokazakifragmentmaturationduringdnareplication AT julianarana highfidelitydnaligationenforcesaccurateokazakifragmentmaturationduringdnareplication AT rscottwilliams highfidelitydnaligationenforcesaccurateokazakifragmentmaturationduringdnareplication AT thomasakunkel highfidelitydnaligationenforcesaccurateokazakifragmentmaturationduringdnareplication
_version_	1724326252993576960

High-fidelity DNA ligation enforces accurate Okazaki fragment maturation during DNA replication

Similar Items