In Vitro Prevention of Salmonella Lipopolysaccharide-Induced Damages in Epithelial Barrier Function by Various Lactobacillus Strains
Background. Lactobacillus shows beneficial anti-inflammatory effects on Salmonella infection. The maintenance of the tight junction (TJ) integrity plays an importance role in avoiding bacterial invasion. Whether Lactobacillus could be used to regulate the TJ protein expression and distribution in in...
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2013-01-01
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Series: | Gastroenterology Research and Practice |
Online Access: | http://dx.doi.org/10.1155/2013/973209 |
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doaj-c71345148c814813927e3b371e0890cd2020-11-24T23:10:00ZengHindawi LimitedGastroenterology Research and Practice1687-61211687-630X2013-01-01201310.1155/2013/973209973209In Vitro Prevention of Salmonella Lipopolysaccharide-Induced Damages in Epithelial Barrier Function by Various Lactobacillus StrainsChun-Yan Yeung0Jen-Shiu Chiang Chiau1Wai-Tao Chan2Chun-Bin Jiang3Mei-Lien Cheng4Hsuan-Liang Liu5Hung-Chang Lee6Division of Gastroenterology and Nutrition, Department of Paediatrics, Mackay Memorial Hospital, Taipei 10449, TaiwanDepartment of Medical Research, Mackay Memorial Hospital, Taipei 25160, TaiwanDivision of Gastroenterology and Nutrition, Department of Paediatrics, Mackay Memorial Hospital, Taipei 10449, TaiwanDivision of Gastroenterology and Nutrition, Department of Paediatrics, Mackay Memorial Hospital, Taipei 10449, TaiwanDepartment of Medical Research, Mackay Memorial Hospital, Taipei 25160, TaiwanInstitute of Biotechnology and Department of Chemical Engineering, National Taipei University of Technology, No. 1, Sec. 3, Chung-Hsiao East Road, Taipei 10608, TaiwanDivision of Gastroenterology and Nutrition, Department of Paediatrics, Mackay Memorial Hospital, No. 690, Sec. 2, Guangfu Road, Hsinchu 30071, TaiwanBackground. Lactobacillus shows beneficial anti-inflammatory effects on Salmonella infection. The maintenance of the tight junction (TJ) integrity plays an importance role in avoiding bacterial invasion. Whether Lactobacillus could be used to regulate the TJ protein expression and distribution in inflamed intestinal epithelial cells was determined. Methods. Using the transwell coculture model, Salmonella lipopolysaccharide (LPS) was apically added to polarized Caco-2 cells cocultured with peripheral blood mononuclear cells in the basolateral compartment. LPS-stimulated Caco-2 cells were incubated with various Lactobacillus strains. TJ integrity was determined by measuring transepithelial electrical resistance across Caco-2 monolayer. Expression and localization of TJ proteins (zonula-occludens- (ZO-) 1) were determined by Western blot and immunofluorescence microscopy. Results. Various strains of Lactobacillus were responsible for the different modulations of cell layer integrity. LPS was specifically able to disrupt epithelial barrier and change the location of ZO-1. Our data demonstrate that Lactobacillus could attenuate the barrier disruption of intestinal epithelial cells caused by Salmonella LPS administration. We showed that Lactobacillus strains are associated with the maintenance of the tight junction integrity and appearance. Conclusion. In this study we provide insight that live probiotics could improve epithelial barrier properties and this may explain the potential mechanism behind their beneficial effect in vivo.http://dx.doi.org/10.1155/2013/973209 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chun-Yan Yeung Jen-Shiu Chiang Chiau Wai-Tao Chan Chun-Bin Jiang Mei-Lien Cheng Hsuan-Liang Liu Hung-Chang Lee |
spellingShingle |
Chun-Yan Yeung Jen-Shiu Chiang Chiau Wai-Tao Chan Chun-Bin Jiang Mei-Lien Cheng Hsuan-Liang Liu Hung-Chang Lee In Vitro Prevention of Salmonella Lipopolysaccharide-Induced Damages in Epithelial Barrier Function by Various Lactobacillus Strains Gastroenterology Research and Practice |
author_facet |
Chun-Yan Yeung Jen-Shiu Chiang Chiau Wai-Tao Chan Chun-Bin Jiang Mei-Lien Cheng Hsuan-Liang Liu Hung-Chang Lee |
author_sort |
Chun-Yan Yeung |
title |
In Vitro Prevention of Salmonella Lipopolysaccharide-Induced Damages in Epithelial Barrier Function by Various Lactobacillus Strains |
title_short |
In Vitro Prevention of Salmonella Lipopolysaccharide-Induced Damages in Epithelial Barrier Function by Various Lactobacillus Strains |
title_full |
In Vitro Prevention of Salmonella Lipopolysaccharide-Induced Damages in Epithelial Barrier Function by Various Lactobacillus Strains |
title_fullStr |
In Vitro Prevention of Salmonella Lipopolysaccharide-Induced Damages in Epithelial Barrier Function by Various Lactobacillus Strains |
title_full_unstemmed |
In Vitro Prevention of Salmonella Lipopolysaccharide-Induced Damages in Epithelial Barrier Function by Various Lactobacillus Strains |
title_sort |
in vitro prevention of salmonella lipopolysaccharide-induced damages in epithelial barrier function by various lactobacillus strains |
publisher |
Hindawi Limited |
series |
Gastroenterology Research and Practice |
issn |
1687-6121 1687-630X |
publishDate |
2013-01-01 |
description |
Background. Lactobacillus shows beneficial anti-inflammatory effects on Salmonella infection. The maintenance of the tight junction (TJ) integrity plays an importance role in avoiding bacterial invasion. Whether Lactobacillus could be used to regulate the TJ protein expression and distribution in inflamed intestinal epithelial cells was determined. Methods. Using the transwell coculture model, Salmonella lipopolysaccharide (LPS) was apically added to polarized Caco-2 cells cocultured with peripheral blood mononuclear cells in the basolateral compartment. LPS-stimulated Caco-2 cells were incubated with various Lactobacillus strains. TJ integrity was determined by measuring transepithelial electrical resistance across Caco-2 monolayer. Expression and localization of TJ proteins (zonula-occludens- (ZO-) 1) were determined by Western blot and immunofluorescence microscopy. Results. Various strains of Lactobacillus were responsible for the different modulations of cell layer integrity. LPS was specifically able to disrupt epithelial barrier and change the location of ZO-1. Our data demonstrate that Lactobacillus could attenuate the barrier disruption of intestinal epithelial cells caused by Salmonella LPS administration. We showed that Lactobacillus strains are associated with the maintenance of the tight junction integrity and appearance. Conclusion. In this study we provide insight that live probiotics could improve epithelial barrier properties and this may explain the potential mechanism behind their beneficial effect in vivo. |
url |
http://dx.doi.org/10.1155/2013/973209 |
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