LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 Methylation

• Main Authors: Long Y, Li H, Jin Z, Zhang X
• Format: Article
• Language: English
• Published: Dove Medical Press 2021-02-01
• Series: Cancer Management and Research
• Subjects: gbm; slc16a1-as1; mir-149; methylation; proliferation
• Online Access: https://www.dovepress.com/lncrna-slc16a1-as1-is-upregulated-in-glioblastoma-and-promotes-cancer--peer-reviewed-article-CMAR

Yinbo Long, Heyang Li, Zhibin Jin, Xiang Zhang Department of Neurosurgery, Cangzhou Central Hospital, Cangzhou City, Hebei Province, 061000, People’s Republic of ChinaCorrespondence: Xiang ZhangDepartment of Neurosurgery, Cangzhou Central Hospital, Cangzhou City, Hebei Province, 061000, People’s Republic of ChinaEmail [e-mail]: LncRNA SLC16A1-AS1 has been characterized as a critical player in lung cancer, while its role in glioblastoma (GBM) is unknown. By analyzing the TCGA dataset, we observed the upregulation of SLC16A1-AS1 expression in GBM. Therefore, we aimed to investigate the role of SLC16A1-AS1 in this cancer.Methods: GBM tissues and paired non-tumor tissues were collected from 62 GBM patients through biopsy. RT-qPCR was performed to determine the expression of SLC16A1-AS1 and miR-149. Linear regression was used to analyze their correlations. The relationship between SLC16A1-AS1 and miR-149 was assessed by gain and loss of function experiments. Methylation-specific PCR (MSP) and bisulfite sequencing PCR (BSP) were performed to analyze the methylation status of miR-149. Cell proliferation was evaluated by CCK-8 assay and colony formation experiments in GBM cells.Results: We found that SLC16A1-AS1 expression was upregulated in GBM tissues, and the upregulated expression of SLC16A1-AS1 predicted poor survival of GBM patients. MiR-149 was downregulated in GBM tissues and inversely correlated with the expression of SLC16A1-AS1. In GBM cells, overexpression of SLC16A1-AS1 downregulated the expression of miR-149 and increased the methylation of miR-149 gene. In cell proliferation and colony formation assay, overexpression of SLC16A1-AS1 reduced the inhibitory effects of miR-149 on GBM cell proliferation.Conclusion: SLC16A1-AS1 may promote GBM cell proliferation by regulating miR-149 methylation. SLC16A1-AS1 can be considered as a potential diagnostic marker in GBM.Keywords: GBM, SLC16A1-AS1, miR-149, methylation, proliferation