Differential Gene Expression in High- and Low-Active Inbred Mice
Numerous candidate genes have been suggested in the recent literature with proposed roles in regulation of voluntary physical activity, with little evidence of these genes’ functional roles. This study compared the haplotype structure and expression profile in skeletal muscle and brain...
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Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2014/361048 |
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doaj-c7236720f7c84dad82cec15b04adcc9d2020-11-24T23:55:02ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/361048361048Differential Gene Expression in High- and Low-Active Inbred MiceMichelle Dawes0Trudy Moore-Harrison1Alicia T. Hamilton2Tyrone Ceaser3Kelli J. Kochan4Penny K. Riggs5J. Timothy Lightfoot6Department of Health and Kinesiology, Texas A&M University, College Station, TX 77843, USADepartment of Kinesiology, University of North Carolina Charlotte, Charlotte, NC 28223, USADepartment of Kinesiology, University of North Carolina Charlotte, Charlotte, NC 28223, USADepartment of Kinesiology, University of North Carolina Charlotte, Charlotte, NC 28223, USADepartment of Animal Science, Texas A&M University, College Station, TX 77843, USADepartment of Animal Science, Texas A&M University, College Station, TX 77843, USADepartment of Health and Kinesiology, Texas A&M University, College Station, TX 77843, USANumerous candidate genes have been suggested in the recent literature with proposed roles in regulation of voluntary physical activity, with little evidence of these genes’ functional roles. This study compared the haplotype structure and expression profile in skeletal muscle and brain of inherently high- (C57L/J) and low- (C3H/HeJ) active mice. Expression of nine candidate genes [Actn2, Actn3, Casq1, Drd2, Lepr, Mc4r, Mstn, Papss2, and Glut4 (a.k.a. Slc2a4)] was evaluated via RT-qPCR. SNPs were observed in regions of Actn2, Casq1, Drd2, Lepr, and Papss2; however, no SNPs were located in coding sequences or associated with any known regulatory sequences. In mice exposed to a running wheel, Casq1 (P=0.0003) and Mstn (P=0.002) transcript levels in the soleus were higher in the low-active mice. However, when these genes were evaluated in naïve animals, differential expression was not observed, demonstrating a training effect. Among naïve mice, no genes in either tissue exhibited differential expression between strains. Considering that no obvious SNP mechanisms were determined or differential expression was observed, our results indicate that genomic structural variation or gene expression data alone is not adequate to establish any of these genes’ candidacy or causality in relation to regulation of physical activity.http://dx.doi.org/10.1155/2014/361048 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michelle Dawes Trudy Moore-Harrison Alicia T. Hamilton Tyrone Ceaser Kelli J. Kochan Penny K. Riggs J. Timothy Lightfoot |
spellingShingle |
Michelle Dawes Trudy Moore-Harrison Alicia T. Hamilton Tyrone Ceaser Kelli J. Kochan Penny K. Riggs J. Timothy Lightfoot Differential Gene Expression in High- and Low-Active Inbred Mice BioMed Research International |
author_facet |
Michelle Dawes Trudy Moore-Harrison Alicia T. Hamilton Tyrone Ceaser Kelli J. Kochan Penny K. Riggs J. Timothy Lightfoot |
author_sort |
Michelle Dawes |
title |
Differential Gene Expression in High- and Low-Active Inbred Mice |
title_short |
Differential Gene Expression in High- and Low-Active Inbred Mice |
title_full |
Differential Gene Expression in High- and Low-Active Inbred Mice |
title_fullStr |
Differential Gene Expression in High- and Low-Active Inbred Mice |
title_full_unstemmed |
Differential Gene Expression in High- and Low-Active Inbred Mice |
title_sort |
differential gene expression in high- and low-active inbred mice |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2014-01-01 |
description |
Numerous candidate genes have been suggested in the recent literature with proposed roles in regulation of voluntary physical activity, with little evidence of these genes’ functional roles.
This study compared the haplotype structure and expression profile in skeletal muscle and brain of inherently high- (C57L/J) and low- (C3H/HeJ) active mice. Expression of nine candidate genes
[Actn2, Actn3, Casq1, Drd2, Lepr, Mc4r, Mstn, Papss2, and Glut4 (a.k.a. Slc2a4)] was evaluated via RT-qPCR. SNPs were observed in regions of
Actn2, Casq1, Drd2, Lepr,
and Papss2; however,
no SNPs were located in coding sequences or associated with any known regulatory sequences. In mice exposed to a running wheel, Casq1 (P=0.0003) and Mstn (P=0.002) transcript levels in the
soleus were higher in the low-active mice. However, when these genes were evaluated in naïve animals, differential expression was not observed, demonstrating a training effect. Among naïve mice,
no genes in either tissue exhibited differential expression between strains. Considering that no obvious SNP mechanisms were determined or differential expression was observed, our results indicate
that genomic structural variation or gene expression data alone is not adequate to establish any of these genes’ candidacy or causality in relation to regulation of physical activity. |
url |
http://dx.doi.org/10.1155/2014/361048 |
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