Phase inversion-based nanoemulsions of medium chain triglyceride as potential drug delivery system for parenteral applications
Lipid nanoemulsions are attractive drug delivery systems for lipophilic drugs. To produce nanoemulsions with droplets of very small diameter (<100 nm), we investigated thermotropic phase transitions as an alternative to the standard procedure of high-pressure homogenization. Employing shock dilut...
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doaj-c73579fdf7ee4547aef82026668936b72020-11-25T01:24:52ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862020-01-0111121322410.3762/bjnano.11.162190-4286-11-16Phase inversion-based nanoemulsions of medium chain triglyceride as potential drug delivery system for parenteral applicationsEike Folker Busmann0Dailén García Martínez1Henrike Lucas2Karsten Mäder3Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle (Saale), GermanyInstitute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle (Saale), GermanyInstitute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle (Saale), GermanyInstitute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle (Saale), GermanyLipid nanoemulsions are attractive drug delivery systems for lipophilic drugs. To produce nanoemulsions with droplets of very small diameter (<100 nm), we investigated thermotropic phase transitions as an alternative to the standard procedure of high-pressure homogenization. Employing shock dilution with ice-cold water during the phase inversion gives the opportunity to produce nanoemulsions without any use of potentially toxic organic solvents. The systematic investigation of the relation of the three involved components surfactant, aqueous phase and lipid phase showed that depending on the ratio of surfactant to lipid the emulsions contained particles of diameters between 16 and 175 nm with narrow polydispersity index distributions and uncharged surfaces. Nanoemulsions with particles of 50 and 100 nm in diameter showed very little toxicity to fibroblast cells in vitro. An unusual, exponential-like nonlinear increase in osmolality was observed with increasing concentration of the nonionic surfactant Kolliphor HS 15. The experimental results indicate, that nanoemulsions with particles of small and tunable size can be easily formed without homogenization by thermal cycling.https://doi.org/10.3762/bjnano.11.16cellular toxicityisotonicitynanoemulsionphase inversionsolvent freesurface properties |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Eike Folker Busmann Dailén García Martínez Henrike Lucas Karsten Mäder |
spellingShingle |
Eike Folker Busmann Dailén García Martínez Henrike Lucas Karsten Mäder Phase inversion-based nanoemulsions of medium chain triglyceride as potential drug delivery system for parenteral applications Beilstein Journal of Nanotechnology cellular toxicity isotonicity nanoemulsion phase inversion solvent free surface properties |
author_facet |
Eike Folker Busmann Dailén García Martínez Henrike Lucas Karsten Mäder |
author_sort |
Eike Folker Busmann |
title |
Phase inversion-based nanoemulsions of medium chain triglyceride as potential drug delivery system for parenteral applications |
title_short |
Phase inversion-based nanoemulsions of medium chain triglyceride as potential drug delivery system for parenteral applications |
title_full |
Phase inversion-based nanoemulsions of medium chain triglyceride as potential drug delivery system for parenteral applications |
title_fullStr |
Phase inversion-based nanoemulsions of medium chain triglyceride as potential drug delivery system for parenteral applications |
title_full_unstemmed |
Phase inversion-based nanoemulsions of medium chain triglyceride as potential drug delivery system for parenteral applications |
title_sort |
phase inversion-based nanoemulsions of medium chain triglyceride as potential drug delivery system for parenteral applications |
publisher |
Beilstein-Institut |
series |
Beilstein Journal of Nanotechnology |
issn |
2190-4286 |
publishDate |
2020-01-01 |
description |
Lipid nanoemulsions are attractive drug delivery systems for lipophilic drugs. To produce nanoemulsions with droplets of very small diameter (<100 nm), we investigated thermotropic phase transitions as an alternative to the standard procedure of high-pressure homogenization. Employing shock dilution with ice-cold water during the phase inversion gives the opportunity to produce nanoemulsions without any use of potentially toxic organic solvents. The systematic investigation of the relation of the three involved components surfactant, aqueous phase and lipid phase showed that depending on the ratio of surfactant to lipid the emulsions contained particles of diameters between 16 and 175 nm with narrow polydispersity index distributions and uncharged surfaces. Nanoemulsions with particles of 50 and 100 nm in diameter showed very little toxicity to fibroblast cells in vitro. An unusual, exponential-like nonlinear increase in osmolality was observed with increasing concentration of the nonionic surfactant Kolliphor HS 15. The experimental results indicate, that nanoemulsions with particles of small and tunable size can be easily formed without homogenization by thermal cycling. |
topic |
cellular toxicity isotonicity nanoemulsion phase inversion solvent free surface properties |
url |
https://doi.org/10.3762/bjnano.11.16 |
work_keys_str_mv |
AT eikefolkerbusmann phaseinversionbasednanoemulsionsofmediumchaintriglycerideaspotentialdrugdeliverysystemforparenteralapplications AT dailengarciamartinez phaseinversionbasednanoemulsionsofmediumchaintriglycerideaspotentialdrugdeliverysystemforparenteralapplications AT henrikelucas phaseinversionbasednanoemulsionsofmediumchaintriglycerideaspotentialdrugdeliverysystemforparenteralapplications AT karstenmader phaseinversionbasednanoemulsionsofmediumchaintriglycerideaspotentialdrugdeliverysystemforparenteralapplications |
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1725116593250238464 |