Brucella Seroprevalence in a High-Risk Population in Greece: A Cross-Sectional Study
Introduction. Brucellosis is a zoonosis with high occupational risk. However, seroprevalence of Brucella antibodies among occupational groups is not known, since studies in endemic countries are rare. Methods. A cross-sectional seroprevalence study was conducted among livestock farmers in an endemic...
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doaj-c73cd79d0f1f48bbb8d2986b9f07e3bb2020-11-25T00:03:25ZengHindawi LimitedInterdisciplinary Perspectives on Infectious Diseases1687-708X1687-70982018-01-01201810.1155/2018/87519218751921Brucella Seroprevalence in a High-Risk Population in Greece: A Cross-Sectional StudyPanagiotis Andriopoulos0Dimitrios Floros1Natalia Gioti2Anargiros Mariolis3Andrea Paola Rojas Gil4Maria Tsironi5Laboratory of Epidemiology and Prevention of Hemoglobinopathies, Acute and Chronic Diseases, Department of Nursing, University of Peloponnese, Valioti & Plateon, 23100 Sparta, Laconia, GreeceLaboratory of Epidemiology and Prevention of Hemoglobinopathies, Acute and Chronic Diseases, Department of Nursing, University of Peloponnese, Valioti & Plateon, 23100 Sparta, Laconia, GreeceLaboratory of Epidemiology and Prevention of Hemoglobinopathies, Acute and Chronic Diseases, Department of Nursing, University of Peloponnese, Valioti & Plateon, 23100 Sparta, Laconia, GreeceAreopoli Health Center, Areopoli 23062, Laconia, GreeceLaboratory of Biology and Biochemistry, Department of Nursing, University of Peloponnese, Valioti & Plateon, 23100 Sparta, Laconia, GreeceLaboratory of Epidemiology and Prevention of Hemoglobinopathies, Acute and Chronic Diseases, Department of Nursing, University of Peloponnese, Valioti & Plateon, 23100 Sparta, Laconia, GreeceIntroduction. Brucellosis is a zoonosis with high occupational risk. However, seroprevalence of Brucella antibodies among occupational groups is not known, since studies in endemic countries are rare. Methods. A cross-sectional seroprevalence study was conducted among livestock farmers in an endemic region in Greece. A low-risk group of individuals that just moved in the region was used as controls. Rose Bengal, Wright standard tube agglutination (STA) tests, and specific IgG and IgM antibodies using ELISA were evaluated; differences and odds ratios were calculated. Results were compared with studies from other endemic regions. Results. 100 livestock farmers and family members and 34 first-year students were enrolled. Rose Bengal results were 18% positive versus 0% (p=0.007); Wright STAs for Brucella melitensis were 8% versus 2.9% (p=0.448) and for Brucella abortus they were 2% versus 2.9% (p=0.588). ELISA IgG was positive in 8% of farmers versus 2.9% of students (p=0.448). Parallel testing with Rose Bengal and ELISA IgG was positive in 3% versus 0% (p=0.571). No significant odds ratios were calculated for Wright STAs and ELISA IgG. Conclusions. Healthy livestock farmers may present with positive Rose Bengal test but this translates to true seroprevalence in only a small proportion. Livestock farmers have no significant seroprevalence that may obscure diagnosis of acute brucellosis in clinical settings.http://dx.doi.org/10.1155/2018/8751921 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Panagiotis Andriopoulos Dimitrios Floros Natalia Gioti Anargiros Mariolis Andrea Paola Rojas Gil Maria Tsironi |
spellingShingle |
Panagiotis Andriopoulos Dimitrios Floros Natalia Gioti Anargiros Mariolis Andrea Paola Rojas Gil Maria Tsironi Brucella Seroprevalence in a High-Risk Population in Greece: A Cross-Sectional Study Interdisciplinary Perspectives on Infectious Diseases |
author_facet |
Panagiotis Andriopoulos Dimitrios Floros Natalia Gioti Anargiros Mariolis Andrea Paola Rojas Gil Maria Tsironi |
author_sort |
Panagiotis Andriopoulos |
title |
Brucella Seroprevalence in a High-Risk Population in Greece: A Cross-Sectional Study |
title_short |
Brucella Seroprevalence in a High-Risk Population in Greece: A Cross-Sectional Study |
title_full |
Brucella Seroprevalence in a High-Risk Population in Greece: A Cross-Sectional Study |
title_fullStr |
Brucella Seroprevalence in a High-Risk Population in Greece: A Cross-Sectional Study |
title_full_unstemmed |
Brucella Seroprevalence in a High-Risk Population in Greece: A Cross-Sectional Study |
title_sort |
brucella seroprevalence in a high-risk population in greece: a cross-sectional study |
publisher |
Hindawi Limited |
series |
Interdisciplinary Perspectives on Infectious Diseases |
issn |
1687-708X 1687-7098 |
publishDate |
2018-01-01 |
description |
Introduction. Brucellosis is a zoonosis with high occupational risk. However, seroprevalence of Brucella antibodies among occupational groups is not known, since studies in endemic countries are rare. Methods. A cross-sectional seroprevalence study was conducted among livestock farmers in an endemic region in Greece. A low-risk group of individuals that just moved in the region was used as controls. Rose Bengal, Wright standard tube agglutination (STA) tests, and specific IgG and IgM antibodies using ELISA were evaluated; differences and odds ratios were calculated. Results were compared with studies from other endemic regions. Results. 100 livestock farmers and family members and 34 first-year students were enrolled. Rose Bengal results were 18% positive versus 0% (p=0.007); Wright STAs for Brucella melitensis were 8% versus 2.9% (p=0.448) and for Brucella abortus they were 2% versus 2.9% (p=0.588). ELISA IgG was positive in 8% of farmers versus 2.9% of students (p=0.448). Parallel testing with Rose Bengal and ELISA IgG was positive in 3% versus 0% (p=0.571). No significant odds ratios were calculated for Wright STAs and ELISA IgG. Conclusions. Healthy livestock farmers may present with positive Rose Bengal test but this translates to true seroprevalence in only a small proportion. Livestock farmers have no significant seroprevalence that may obscure diagnosis of acute brucellosis in clinical settings. |
url |
http://dx.doi.org/10.1155/2018/8751921 |
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