Cell migration induced by Leishmania (Leishmania) amazonensis, Leishmania (Leishmania) major and Leishmania (Viannia) braziliensis into the peritoneal cavity of BALB/c mice
In American cutaneous leishmaniasis, the initial infection phase is characterized by recruitment of neutrophils and monocytes. The migration of these cells in response to the presence of Leishmania in the peritoneum of affected animals remains unclear. The objective of this study was to investigate...
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doaj-c73e654783cc4d15bb374f61745e355f2020-11-25T01:48:52ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992010-01-0116117017710.1590/S1678-91992010000100017Cell migration induced by Leishmania (Leishmania) amazonensis, Leishmania (Leishmania) major and Leishmania (Viannia) braziliensis into the peritoneal cavity of BALB/c miceDT WakimotoKV GasparetoTGV SilveiraMVC LonardoniSMA AristidesIn American cutaneous leishmaniasis, the initial infection phase is characterized by recruitment of neutrophils and monocytes. The migration of these cells in response to the presence of Leishmania in the peritoneum of affected animals remains unclear. The objective of this study was to investigate cell migration to the peritoneum of BALB/c mice after infection with Leishmania (Leishmania) amazonensis, Leishmania (Viannia) braziliensis and Leishmania (Leishmania) major. Initially, Leishmania spp. was intraperitoneally inoculated in five groups of six animals each and the cell migration was analyzed 0, 3, 6, 12, 24 and 48 hours after infection. Different cell counts were performed with a staining kit and showed a higher percentage of polymorphonuclear than mononuclear cells in all three species studied. The total cell count revealed peak migration in L. (L.) amazonensis and L. (L.) major at six hours, and in L. (V.) braziliensis at 12 hours. These results suggest that factors released from different cell types probably act by attracting polymorphonuclear cells, with the peak migration most likely depending on the species of Leishmania inoculated into the host.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100017BALB/c miceLeishmania spp.American cutaneous leishmaniasis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
DT Wakimoto KV Gaspareto TGV Silveira MVC Lonardoni SMA Aristides |
spellingShingle |
DT Wakimoto KV Gaspareto TGV Silveira MVC Lonardoni SMA Aristides Cell migration induced by Leishmania (Leishmania) amazonensis, Leishmania (Leishmania) major and Leishmania (Viannia) braziliensis into the peritoneal cavity of BALB/c mice Journal of Venomous Animals and Toxins including Tropical Diseases BALB/c mice Leishmania spp. American cutaneous leishmaniasis |
author_facet |
DT Wakimoto KV Gaspareto TGV Silveira MVC Lonardoni SMA Aristides |
author_sort |
DT Wakimoto |
title |
Cell migration induced by Leishmania (Leishmania) amazonensis, Leishmania (Leishmania) major and Leishmania (Viannia) braziliensis into the peritoneal cavity of BALB/c mice |
title_short |
Cell migration induced by Leishmania (Leishmania) amazonensis, Leishmania (Leishmania) major and Leishmania (Viannia) braziliensis into the peritoneal cavity of BALB/c mice |
title_full |
Cell migration induced by Leishmania (Leishmania) amazonensis, Leishmania (Leishmania) major and Leishmania (Viannia) braziliensis into the peritoneal cavity of BALB/c mice |
title_fullStr |
Cell migration induced by Leishmania (Leishmania) amazonensis, Leishmania (Leishmania) major and Leishmania (Viannia) braziliensis into the peritoneal cavity of BALB/c mice |
title_full_unstemmed |
Cell migration induced by Leishmania (Leishmania) amazonensis, Leishmania (Leishmania) major and Leishmania (Viannia) braziliensis into the peritoneal cavity of BALB/c mice |
title_sort |
cell migration induced by leishmania (leishmania) amazonensis, leishmania (leishmania) major and leishmania (viannia) braziliensis into the peritoneal cavity of balb/c mice |
publisher |
SciELO |
series |
Journal of Venomous Animals and Toxins including Tropical Diseases |
issn |
1678-9199 |
publishDate |
2010-01-01 |
description |
In American cutaneous leishmaniasis, the initial infection phase is characterized by recruitment of neutrophils and monocytes. The migration of these cells in response to the presence of Leishmania in the peritoneum of affected animals remains unclear. The objective of this study was to investigate cell migration to the peritoneum of BALB/c mice after infection with Leishmania (Leishmania) amazonensis, Leishmania (Viannia) braziliensis and Leishmania (Leishmania) major. Initially, Leishmania spp. was intraperitoneally inoculated in five groups of six animals each and the cell migration was analyzed 0, 3, 6, 12, 24 and 48 hours after infection. Different cell counts were performed with a staining kit and showed a higher percentage of polymorphonuclear than mononuclear cells in all three species studied. The total cell count revealed peak migration in L. (L.) amazonensis and L. (L.) major at six hours, and in L. (V.) braziliensis at 12 hours. These results suggest that factors released from different cell types probably act by attracting polymorphonuclear cells, with the peak migration most likely depending on the species of Leishmania inoculated into the host. |
topic |
BALB/c mice Leishmania spp. American cutaneous leishmaniasis |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100017 |
work_keys_str_mv |
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