Comparison of customized spin-column and salt-precipitation finger-prick blood DNA extraction

• Main Authors: Jun‑Jie Poh, Samuel Ken‑En Gan
• Format: Article
• Language: English
• Published: Portland Press, Biochemical Society 2014-10-01
• Series: Bioscience Reports
• Subjects: column-based purifications; gDNA extraction; nucleated blood; PCR; salt-precipitation; whole blood
• Online Access: http://www.bioscirep.org/bsr/034/e145/bsr034e145.htm

gDNA (genomic DNA extraction from blood is a fundamental process in many diagnostic, identification and research applications. Numerous extraction methods have been reported and are available commercially. However, there is insufficient understanding of the impact of chemical buffers on DNA yield from either whole or nucleated blood. Moreover, these commercial kits are often costly, constraining less well-funded laboratories to traditional and more cost-effective salt-precipitation methods. Towards this, we compared a salt-precipitation and a customized cost-effective spin-column-based method, studying the impact of different chemical constituents on the yields. This customized method resulted in a shortening of the extraction process, higher gDNA yields, and more successful PCR amplification of gDNA genes compared with the salt-precipitation method. Optimizing different chemical buffers on whole- and nucleated blood materials further revealed that certain chemicals boosted extractions from whole- but not nucleated blood. These findings may be useful to laboratories that do not have ready access to commercial kits, and improve their nucleic acid extractions from blood economically.