Effect of freezing conservation time on loquat (Eriobotrya japonica) pollen germination
Aim of study: Several studies point out that storage at -20 ºC is a suitable method for preserving pollen of many species in the long term. Part of those studies indicate the total storage time at which these conditions are optimal. However, we have found a lack of information about the freezing tim...
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Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2020-12-01
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doaj-c782aa18694d4dbbb02731b0652579d62021-01-04T21:55:09ZengInstituto Nacional de Investigación y Tecnología Agraria y AlimentariaSpanish Journal of Agricultural Research2171-92922020-12-01183e0804e080410.5424/sjar/2020183-166263053Effect of freezing conservation time on loquat (Eriobotrya japonica) pollen germinationRoberto Beltrán0Nuria Cebrián1Carlos Zornoza2Alfonso Garmendia3Hugo B. Merle4Universitat Politècnica de València, Dept. Ecosistemas Agroforestales. Camino de Vera s/n, 46022 ValenciaUniversitat Politècnica de València, Dept. Ecosistemas Agroforestales. Camino de Vera s/n, 46022 ValenciaS.A. Explotaciones Agrícolas Serrano (SAEAS). c/ En Sanz 5-1. 46001 ValenciaUniversitat Politècnica de València, Instituto Agroforestal Mediterráneo, 46022 ValenciaUniversitat Politècnica de València, Dept. Ecosistemas Agroforestales. Camino de Vera s/n, 46022 ValenciaAim of study: Several studies point out that storage at -20 ºC is a suitable method for preserving pollen of many species in the long term. Part of those studies indicate the total storage time at which these conditions are optimal. However, we have found a lack of information about the freezing time conditions and incubation temperature of loquat pollen. The main objective of this study was to evaluate the effect of the -20 ºC conservation temperature on loquat (Eriobotrya japonica (Thunb.) Lindl.) pollen. Area of study: The study was conducted in Montserrat (Valencia, Spain). Material and methods: Loquat flowers were collected in November 2017 and stored at -20 ºC for three time periods: 4 (T1), 6 (T2) and 8 (T3) months. Subsequently, pollen grains were incubated at different temperatures for 72 h. We analyzed (i) the effect of freezing conservation time; (ii) the effect of incubation temperature on germination; (iii) the interaction between these two factors. Main results: T1 showed higher germination percentage and tube length values (mean and maximum) than T2 and T3. The highest germination percentage (52.77%) was detected for T1 at an incubation temperature of 25 ºC. The interaction between freezing time and incubation temperature showed more consistent results for T1 than for T2 and T3. Research highlights: This suggests that storing at -20 ºC for more than 4 months affects pollen grain and reduces germination and pollen growth. Therefore, -20 ºC loquat pollen storage should not exceed 4 months.https://revistas.inia.es/index.php/sjar/article/view/16626pollen germination ratepollen tube lengthfruit treerosaceaepollen conservation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Roberto Beltrán Nuria Cebrián Carlos Zornoza Alfonso Garmendia Hugo B. Merle |
spellingShingle |
Roberto Beltrán Nuria Cebrián Carlos Zornoza Alfonso Garmendia Hugo B. Merle Effect of freezing conservation time on loquat (Eriobotrya japonica) pollen germination Spanish Journal of Agricultural Research pollen germination rate pollen tube length fruit tree rosaceae pollen conservation |
author_facet |
Roberto Beltrán Nuria Cebrián Carlos Zornoza Alfonso Garmendia Hugo B. Merle |
author_sort |
Roberto Beltrán |
title |
Effect of freezing conservation time on loquat (Eriobotrya japonica) pollen germination |
title_short |
Effect of freezing conservation time on loquat (Eriobotrya japonica) pollen germination |
title_full |
Effect of freezing conservation time on loquat (Eriobotrya japonica) pollen germination |
title_fullStr |
Effect of freezing conservation time on loquat (Eriobotrya japonica) pollen germination |
title_full_unstemmed |
Effect of freezing conservation time on loquat (Eriobotrya japonica) pollen germination |
title_sort |
effect of freezing conservation time on loquat (eriobotrya japonica) pollen germination |
publisher |
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria |
series |
Spanish Journal of Agricultural Research |
issn |
2171-9292 |
publishDate |
2020-12-01 |
description |
Aim of study: Several studies point out that storage at -20 ºC is a suitable method for preserving pollen of many species in the long term. Part of those studies indicate the total storage time at which these conditions are optimal. However, we have found a lack of information about the freezing time conditions and incubation temperature of loquat pollen. The main objective of this study was to evaluate the effect of the -20 ºC conservation temperature on loquat (Eriobotrya japonica (Thunb.) Lindl.) pollen.
Area of study: The study was conducted in Montserrat (Valencia, Spain).
Material and methods: Loquat flowers were collected in November 2017 and stored at -20 ºC for three time periods: 4 (T1), 6 (T2) and 8 (T3) months. Subsequently, pollen grains were incubated at different temperatures for 72 h. We analyzed (i) the effect of freezing conservation time; (ii) the effect of incubation temperature on germination; (iii) the interaction between these two factors.
Main results: T1 showed higher germination percentage and tube length values (mean and maximum) than T2 and T3. The highest germination percentage (52.77%) was detected for T1 at an incubation temperature of 25 ºC. The interaction between freezing time and incubation temperature showed more consistent results for T1 than for T2 and T3.
Research highlights: This suggests that storing at -20 ºC for more than 4 months affects pollen grain and reduces germination and pollen growth. Therefore, -20 ºC loquat pollen storage should not exceed 4 months. |
topic |
pollen germination rate pollen tube length fruit tree rosaceae pollen conservation |
url |
https://revistas.inia.es/index.php/sjar/article/view/16626 |
work_keys_str_mv |
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