Inflammation and regulatory T cell genes are differentially expressed in peripheral blood mononuclear cells of Parkinson’s disease patients
Abstract Our aim was to identify the differentially expressed genes (DEGs) in peripheral blood mononuclear cells (PBMC) of Parkinson’s disease (PD) patients and healthy controls by microarray technology and analysis of related molecular pathways by functional annotation. Thirty PD patients and 30 co...
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doaj-c78f37eab31e4824abac58fb8b4fb7f12021-01-31T16:27:03ZengNature Publishing GroupScientific Reports2045-23222021-01-0111111610.1038/s41598-021-81961-7Inflammation and regulatory T cell genes are differentially expressed in peripheral blood mononuclear cells of Parkinson’s disease patientsZerrin Karaaslan0Özlem Timirci Kahraman1Elif Şanlı2Hayriye Arzu Ergen3Canan Ulusoy4Başar Bilgiç5Vuslat Yılmaz6Erdem Tüzün7Haşmet Ayhan Hanağası8Cem İsmail Küçükali9Department of Neuroscience, Aziz Sancar Institute of Experimental Medicine, Istanbul UniversityDepartment of Molecular Medicine, Aziz Sancar Institute of Experimental Medicine, Istanbul UniversityDepartment of Neuroscience, Aziz Sancar Institute of Experimental Medicine, Istanbul UniversityDepartment of Molecular Medicine, Aziz Sancar Institute of Experimental Medicine, Istanbul UniversityDepartment of Neuroscience, Aziz Sancar Institute of Experimental Medicine, Istanbul UniversityDepartment of Neurology, Istanbul Faculty of Medicine, Istanbul UniversityDepartment of Neuroscience, Aziz Sancar Institute of Experimental Medicine, Istanbul UniversityDepartment of Neuroscience, Aziz Sancar Institute of Experimental Medicine, Istanbul UniversityDepartment of Neurology, Istanbul Faculty of Medicine, Istanbul UniversityDepartment of Neuroscience, Aziz Sancar Institute of Experimental Medicine, Istanbul UniversityAbstract Our aim was to identify the differentially expressed genes (DEGs) in peripheral blood mononuclear cells (PBMC) of Parkinson’s disease (PD) patients and healthy controls by microarray technology and analysis of related molecular pathways by functional annotation. Thirty PD patients and 30 controls were enrolled. Agilent Human 8X60 K Oligo Microarray was used for gene level expression identification. Gene ontology and pathway enrichment analyses were used for functional annotation of DEGs. Protein–protein interaction analyses were performed with STRING. Expression levels of randomly selected DEGs were quantified by real time quantitative polymerase chain reaction (RT-PCR) for validation. Flow cytometry was done to determine frequency of regulatory T cells (Tregs) in PBMC. A total of 361 DEGs (143 upregulated and 218 downregulated) were identified after GeneSpring analysis. DEGs were involved in 28 biological processes, 12 cellular components and 26 molecular functions. Pathway analyses demonstrated that upregulated genes mainly enriched in p53 (CASP3, TSC2, ATR, MDM4, CCNG1) and PI3K/Akt (IL2RA, IL4R, TSC2, VEGFA, PKN2, PIK3CA, ITGA4, BCL2L11) signaling pathways. TP53 and PIK3CA were identified as most significant hub proteins. Expression profiles obtained by RT-PCR were consistent with microarray findings. PD patients showed increased proportions of CD49d+ Tregs, which correlated with disability scores. Survival pathway genes were upregulated putatively to compensate neuronal degeneration. Bioinformatics analysis showed an association between survival and inflammation genes. Increased CD49d+ Treg ratios might signify the effort of the immune system to suppress ongoing neuroinflammation.https://doi.org/10.1038/s41598-021-81961-7 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zerrin Karaaslan Özlem Timirci Kahraman Elif Şanlı Hayriye Arzu Ergen Canan Ulusoy Başar Bilgiç Vuslat Yılmaz Erdem Tüzün Haşmet Ayhan Hanağası Cem İsmail Küçükali |
spellingShingle |
Zerrin Karaaslan Özlem Timirci Kahraman Elif Şanlı Hayriye Arzu Ergen Canan Ulusoy Başar Bilgiç Vuslat Yılmaz Erdem Tüzün Haşmet Ayhan Hanağası Cem İsmail Küçükali Inflammation and regulatory T cell genes are differentially expressed in peripheral blood mononuclear cells of Parkinson’s disease patients Scientific Reports |
author_facet |
Zerrin Karaaslan Özlem Timirci Kahraman Elif Şanlı Hayriye Arzu Ergen Canan Ulusoy Başar Bilgiç Vuslat Yılmaz Erdem Tüzün Haşmet Ayhan Hanağası Cem İsmail Küçükali |
author_sort |
Zerrin Karaaslan |
title |
Inflammation and regulatory T cell genes are differentially expressed in peripheral blood mononuclear cells of Parkinson’s disease patients |
title_short |
Inflammation and regulatory T cell genes are differentially expressed in peripheral blood mononuclear cells of Parkinson’s disease patients |
title_full |
Inflammation and regulatory T cell genes are differentially expressed in peripheral blood mononuclear cells of Parkinson’s disease patients |
title_fullStr |
Inflammation and regulatory T cell genes are differentially expressed in peripheral blood mononuclear cells of Parkinson’s disease patients |
title_full_unstemmed |
Inflammation and regulatory T cell genes are differentially expressed in peripheral blood mononuclear cells of Parkinson’s disease patients |
title_sort |
inflammation and regulatory t cell genes are differentially expressed in peripheral blood mononuclear cells of parkinson’s disease patients |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-01-01 |
description |
Abstract Our aim was to identify the differentially expressed genes (DEGs) in peripheral blood mononuclear cells (PBMC) of Parkinson’s disease (PD) patients and healthy controls by microarray technology and analysis of related molecular pathways by functional annotation. Thirty PD patients and 30 controls were enrolled. Agilent Human 8X60 K Oligo Microarray was used for gene level expression identification. Gene ontology and pathway enrichment analyses were used for functional annotation of DEGs. Protein–protein interaction analyses were performed with STRING. Expression levels of randomly selected DEGs were quantified by real time quantitative polymerase chain reaction (RT-PCR) for validation. Flow cytometry was done to determine frequency of regulatory T cells (Tregs) in PBMC. A total of 361 DEGs (143 upregulated and 218 downregulated) were identified after GeneSpring analysis. DEGs were involved in 28 biological processes, 12 cellular components and 26 molecular functions. Pathway analyses demonstrated that upregulated genes mainly enriched in p53 (CASP3, TSC2, ATR, MDM4, CCNG1) and PI3K/Akt (IL2RA, IL4R, TSC2, VEGFA, PKN2, PIK3CA, ITGA4, BCL2L11) signaling pathways. TP53 and PIK3CA were identified as most significant hub proteins. Expression profiles obtained by RT-PCR were consistent with microarray findings. PD patients showed increased proportions of CD49d+ Tregs, which correlated with disability scores. Survival pathway genes were upregulated putatively to compensate neuronal degeneration. Bioinformatics analysis showed an association between survival and inflammation genes. Increased CD49d+ Treg ratios might signify the effort of the immune system to suppress ongoing neuroinflammation. |
url |
https://doi.org/10.1038/s41598-021-81961-7 |
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