Comparative Analysis of Carbohydrates, Nucleosides and Amino Acids in Different Parts of <i>Trichosanthes kirilowii</i> Maxim. by (Ultra) High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry and Evaporative Light Scattering Detector Methods
<i>Trichosanthes kirilowii</i> Maxim. is one of the original plants for traditional Chinese medicines Trichosanthis Fructus, Trichosanthis Semen, Trichosanthis Pericarpium and Trichosanthis Radix. Amino acids, nucleosides and carbohydrates are usually considered to have nutritional value...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-04-01
|
Series: | Molecules |
Subjects: | |
Online Access: | https://www.mdpi.com/1420-3049/24/7/1440 |
id |
doaj-c792a74ae442441eba1589633bccbab2 |
---|---|
record_format |
Article |
spelling |
doaj-c792a74ae442441eba1589633bccbab22020-11-24T21:53:47ZengMDPI AGMolecules1420-30492019-04-01247144010.3390/molecules24071440molecules24071440Comparative Analysis of Carbohydrates, Nucleosides and Amino Acids in Different Parts of <i>Trichosanthes kirilowii</i> Maxim. by (Ultra) High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry and Evaporative Light Scattering Detector MethodsHuang-qin Zhang0Pei Liu1Jin-ao Duan2Ling Dong3Er-xin Shang4Da-wei Qian5Zhen-hua Zhu6Hui-wei Li7Wei-wen Li8Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaInstitute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230001, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaInstitute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230001, China<i>Trichosanthes kirilowii</i> Maxim. is one of the original plants for traditional Chinese medicines Trichosanthis Fructus, Trichosanthis Semen, Trichosanthis Pericarpium and Trichosanthis Radix. Amino acids, nucleosides and carbohydrates are usually considered to have nutritional value and health-care efficacy. In this study, methods involving high-performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD), UV-visible spectrophotometry and ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) were established for quantifying carbohydrates (fructose, glucose, stachyose, raffinose and polysaccharide), fourteen nucleosides and twenty one amino acids. Moreover, sixty-three samples from nine different parts, including pericarp, seed, fruit pulp, stem, leaf, main root, main root bark, lateral root and lateral root bark of <i>T. kirilowii</i> from different cultivated varieties were examined. The established methods were validated with good linearity, precision, repeatability, stability, and recovery. The results showed that the average content of total amino acids in roots (15.39 mg/g) and root barks (16.38 mg/g) were relatively higher than for others. Contents of nucleosides in all parts of <i>T. kirilowii</i> were below 1.5 mg/g. For carbohydrates, fruit pulp has a higher content than others for glucose (22.91%), fructose (20.63%) and polysaccharides (27.29%). By using partial least-squared discriminate analysis (PLS-DA), Variable importance in the projection (VIP) plots and analysis of variance (ANOVA) analysis, the characteristic components of the different organs (fruit, stems and leaves, roots) were found. This analysis suggested there were potential medicinal and nutritive health care values in various parts of the <i>T. kirilowii</i>, which provided valuable information for the development and utilization of <i>T. kirilowii</i>.https://www.mdpi.com/1420-3049/24/7/1440<i>Trichosanthes kirilowii</i> Maximdifferent partscarbohydratenucleosidesamino acidsresource utilization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huang-qin Zhang Pei Liu Jin-ao Duan Ling Dong Er-xin Shang Da-wei Qian Zhen-hua Zhu Hui-wei Li Wei-wen Li |
spellingShingle |
Huang-qin Zhang Pei Liu Jin-ao Duan Ling Dong Er-xin Shang Da-wei Qian Zhen-hua Zhu Hui-wei Li Wei-wen Li Comparative Analysis of Carbohydrates, Nucleosides and Amino Acids in Different Parts of <i>Trichosanthes kirilowii</i> Maxim. by (Ultra) High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry and Evaporative Light Scattering Detector Methods Molecules <i>Trichosanthes kirilowii</i> Maxim different parts carbohydrate nucleosides amino acids resource utilization |
author_facet |
Huang-qin Zhang Pei Liu Jin-ao Duan Ling Dong Er-xin Shang Da-wei Qian Zhen-hua Zhu Hui-wei Li Wei-wen Li |
author_sort |
Huang-qin Zhang |
title |
Comparative Analysis of Carbohydrates, Nucleosides and Amino Acids in Different Parts of <i>Trichosanthes kirilowii</i> Maxim. by (Ultra) High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry and Evaporative Light Scattering Detector Methods |
title_short |
Comparative Analysis of Carbohydrates, Nucleosides and Amino Acids in Different Parts of <i>Trichosanthes kirilowii</i> Maxim. by (Ultra) High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry and Evaporative Light Scattering Detector Methods |
title_full |
Comparative Analysis of Carbohydrates, Nucleosides and Amino Acids in Different Parts of <i>Trichosanthes kirilowii</i> Maxim. by (Ultra) High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry and Evaporative Light Scattering Detector Methods |
title_fullStr |
Comparative Analysis of Carbohydrates, Nucleosides and Amino Acids in Different Parts of <i>Trichosanthes kirilowii</i> Maxim. by (Ultra) High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry and Evaporative Light Scattering Detector Methods |
title_full_unstemmed |
Comparative Analysis of Carbohydrates, Nucleosides and Amino Acids in Different Parts of <i>Trichosanthes kirilowii</i> Maxim. by (Ultra) High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry and Evaporative Light Scattering Detector Methods |
title_sort |
comparative analysis of carbohydrates, nucleosides and amino acids in different parts of <i>trichosanthes kirilowii</i> maxim. by (ultra) high-performance liquid chromatography coupled with tandem mass spectrometry and evaporative light scattering detector methods |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2019-04-01 |
description |
<i>Trichosanthes kirilowii</i> Maxim. is one of the original plants for traditional Chinese medicines Trichosanthis Fructus, Trichosanthis Semen, Trichosanthis Pericarpium and Trichosanthis Radix. Amino acids, nucleosides and carbohydrates are usually considered to have nutritional value and health-care efficacy. In this study, methods involving high-performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD), UV-visible spectrophotometry and ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) were established for quantifying carbohydrates (fructose, glucose, stachyose, raffinose and polysaccharide), fourteen nucleosides and twenty one amino acids. Moreover, sixty-three samples from nine different parts, including pericarp, seed, fruit pulp, stem, leaf, main root, main root bark, lateral root and lateral root bark of <i>T. kirilowii</i> from different cultivated varieties were examined. The established methods were validated with good linearity, precision, repeatability, stability, and recovery. The results showed that the average content of total amino acids in roots (15.39 mg/g) and root barks (16.38 mg/g) were relatively higher than for others. Contents of nucleosides in all parts of <i>T. kirilowii</i> were below 1.5 mg/g. For carbohydrates, fruit pulp has a higher content than others for glucose (22.91%), fructose (20.63%) and polysaccharides (27.29%). By using partial least-squared discriminate analysis (PLS-DA), Variable importance in the projection (VIP) plots and analysis of variance (ANOVA) analysis, the characteristic components of the different organs (fruit, stems and leaves, roots) were found. This analysis suggested there were potential medicinal and nutritive health care values in various parts of the <i>T. kirilowii</i>, which provided valuable information for the development and utilization of <i>T. kirilowii</i>. |
topic |
<i>Trichosanthes kirilowii</i> Maxim different parts carbohydrate nucleosides amino acids resource utilization |
url |
https://www.mdpi.com/1420-3049/24/7/1440 |
work_keys_str_mv |
AT huangqinzhang comparativeanalysisofcarbohydratesnucleosidesandaminoacidsindifferentpartsofitrichosantheskirilowiiimaximbyultrahighperformanceliquidchromatographycoupledwithtandemmassspectrometryandevaporativelightscatteringdetectormethods AT peiliu comparativeanalysisofcarbohydratesnucleosidesandaminoacidsindifferentpartsofitrichosantheskirilowiiimaximbyultrahighperformanceliquidchromatographycoupledwithtandemmassspectrometryandevaporativelightscatteringdetectormethods AT jinaoduan comparativeanalysisofcarbohydratesnucleosidesandaminoacidsindifferentpartsofitrichosantheskirilowiiimaximbyultrahighperformanceliquidchromatographycoupledwithtandemmassspectrometryandevaporativelightscatteringdetectormethods AT lingdong comparativeanalysisofcarbohydratesnucleosidesandaminoacidsindifferentpartsofitrichosantheskirilowiiimaximbyultrahighperformanceliquidchromatographycoupledwithtandemmassspectrometryandevaporativelightscatteringdetectormethods AT erxinshang comparativeanalysisofcarbohydratesnucleosidesandaminoacidsindifferentpartsofitrichosantheskirilowiiimaximbyultrahighperformanceliquidchromatographycoupledwithtandemmassspectrometryandevaporativelightscatteringdetectormethods AT daweiqian comparativeanalysisofcarbohydratesnucleosidesandaminoacidsindifferentpartsofitrichosantheskirilowiiimaximbyultrahighperformanceliquidchromatographycoupledwithtandemmassspectrometryandevaporativelightscatteringdetectormethods AT zhenhuazhu comparativeanalysisofcarbohydratesnucleosidesandaminoacidsindifferentpartsofitrichosantheskirilowiiimaximbyultrahighperformanceliquidchromatographycoupledwithtandemmassspectrometryandevaporativelightscatteringdetectormethods AT huiweili comparativeanalysisofcarbohydratesnucleosidesandaminoacidsindifferentpartsofitrichosantheskirilowiiimaximbyultrahighperformanceliquidchromatographycoupledwithtandemmassspectrometryandevaporativelightscatteringdetectormethods AT weiwenli comparativeanalysisofcarbohydratesnucleosidesandaminoacidsindifferentpartsofitrichosantheskirilowiiimaximbyultrahighperformanceliquidchromatographycoupledwithtandemmassspectrometryandevaporativelightscatteringdetectormethods |
_version_ |
1725870135354327040 |