RNA sequencing identifies common pathways between cigarette smoke exposure and replicative senescence in human airway epithelia
Abstract Background Aging is affected by genetic and environmental factors, and cigarette smoking is strongly associated with accumulation of senescent cells. In this study, we wanted to identify genes that may potentially be beneficial for cell survival in response to cigarette smoke and thereby ma...
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doaj-c7aa9ac2c59a48a6934f46a03d353efd2020-11-25T01:38:08ZengBMCBMC Genomics1471-21642019-01-0120111210.1186/s12864-018-5409-zRNA sequencing identifies common pathways between cigarette smoke exposure and replicative senescence in human airway epitheliaHannah Voic0Xiuying Li1Jun-Ho Jang2Chunbin Zou3Prithu Sundd4Jonathan Alder5Mauricio Rojas6Divay Chandra7Scott Randell8Rama K. Mallampalli9Yohannes Tesfaigzi10Tyrone Ryba11Toru Nyunoya12Division of Natural Sciences, New College of FloridaDepartment of Medicine, University of PittsburghCardiovascular Institute, Department of Medicine, Allegheny Health NetworkDepartment of Medicine, University of PittsburghVascular Medicine Institute, Department of Medicine, University of PittsburghDepartment of Medicine, University of PittsburghDepartment of Medicine, University of PittsburghDepartment of Medicine, University of PittsburghDepartment of Cell and Molecular Physiology, University of North CarolinaDepartment of Medicine, University of PittsburghLovelace Respiratory Research Institute, COPD programDivision of Natural Sciences, New College of FloridaDepartment of Medicine, University of PittsburghAbstract Background Aging is affected by genetic and environmental factors, and cigarette smoking is strongly associated with accumulation of senescent cells. In this study, we wanted to identify genes that may potentially be beneficial for cell survival in response to cigarette smoke and thereby may contribute to development of cellular senescence. Results Primary human bronchial epithelial cells from five healthy donors were cultured, treated with or without 1.5% cigarette smoke extract (CSE) for 24 h or were passaged into replicative senescence. Transcriptome changes were monitored using RNA-seq in CSE and non-CSE exposed cells and those passaged into replicative senescence. We found that, among 1534 genes differentially regulated during senescence and 599 after CSE exposure, 243 were altered in both conditions, representing strong enrichment. Pathways and gene sets overrepresented in both conditions belonged to cellular processes that regulate reactive oxygen species, proteasome degradation, and NF-κB signaling. Conclusions Our results offer insights into gene expression responses during cellular aging and cigarette smoke exposure, and identify potential molecular pathways that are altered by cigarette smoke and may also promote airway epithelial cell senescence.http://link.springer.com/article/10.1186/s12864-018-5409-zReplicative senescencePrimary human bronchial epithelial cellsRNA-seqCigarette smoke |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hannah Voic Xiuying Li Jun-Ho Jang Chunbin Zou Prithu Sundd Jonathan Alder Mauricio Rojas Divay Chandra Scott Randell Rama K. Mallampalli Yohannes Tesfaigzi Tyrone Ryba Toru Nyunoya |
spellingShingle |
Hannah Voic Xiuying Li Jun-Ho Jang Chunbin Zou Prithu Sundd Jonathan Alder Mauricio Rojas Divay Chandra Scott Randell Rama K. Mallampalli Yohannes Tesfaigzi Tyrone Ryba Toru Nyunoya RNA sequencing identifies common pathways between cigarette smoke exposure and replicative senescence in human airway epithelia BMC Genomics Replicative senescence Primary human bronchial epithelial cells RNA-seq Cigarette smoke |
author_facet |
Hannah Voic Xiuying Li Jun-Ho Jang Chunbin Zou Prithu Sundd Jonathan Alder Mauricio Rojas Divay Chandra Scott Randell Rama K. Mallampalli Yohannes Tesfaigzi Tyrone Ryba Toru Nyunoya |
author_sort |
Hannah Voic |
title |
RNA sequencing identifies common pathways between cigarette smoke exposure and replicative senescence in human airway epithelia |
title_short |
RNA sequencing identifies common pathways between cigarette smoke exposure and replicative senescence in human airway epithelia |
title_full |
RNA sequencing identifies common pathways between cigarette smoke exposure and replicative senescence in human airway epithelia |
title_fullStr |
RNA sequencing identifies common pathways between cigarette smoke exposure and replicative senescence in human airway epithelia |
title_full_unstemmed |
RNA sequencing identifies common pathways between cigarette smoke exposure and replicative senescence in human airway epithelia |
title_sort |
rna sequencing identifies common pathways between cigarette smoke exposure and replicative senescence in human airway epithelia |
publisher |
BMC |
series |
BMC Genomics |
issn |
1471-2164 |
publishDate |
2019-01-01 |
description |
Abstract Background Aging is affected by genetic and environmental factors, and cigarette smoking is strongly associated with accumulation of senescent cells. In this study, we wanted to identify genes that may potentially be beneficial for cell survival in response to cigarette smoke and thereby may contribute to development of cellular senescence. Results Primary human bronchial epithelial cells from five healthy donors were cultured, treated with or without 1.5% cigarette smoke extract (CSE) for 24 h or were passaged into replicative senescence. Transcriptome changes were monitored using RNA-seq in CSE and non-CSE exposed cells and those passaged into replicative senescence. We found that, among 1534 genes differentially regulated during senescence and 599 after CSE exposure, 243 were altered in both conditions, representing strong enrichment. Pathways and gene sets overrepresented in both conditions belonged to cellular processes that regulate reactive oxygen species, proteasome degradation, and NF-κB signaling. Conclusions Our results offer insights into gene expression responses during cellular aging and cigarette smoke exposure, and identify potential molecular pathways that are altered by cigarette smoke and may also promote airway epithelial cell senescence. |
topic |
Replicative senescence Primary human bronchial epithelial cells RNA-seq Cigarette smoke |
url |
http://link.springer.com/article/10.1186/s12864-018-5409-z |
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