A molasses habitat-derived fungus Aspergillus tubingensis XG21 with high β-fructofuranosidase activity and its potential use for fructooligosaccharides production
Abstract The industrial microorganisms used for fructooligosaccharides (FOS) synthesis are generally fermented with sucrose as carbon source to induce the production of β-fructofuranosidase (FFase) having transfructosylation activity. Consequently, isolation of novel FFase producers from a sucrose-e...
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doaj-c7b040ee5e4341949e898207f54021562020-11-24T21:13:29ZengSpringerOpenAMB Express2191-08552017-06-01711910.1186/s13568-017-0428-8A molasses habitat-derived fungus Aspergillus tubingensis XG21 with high β-fructofuranosidase activity and its potential use for fructooligosaccharides productionYijia Xie0Huanxia Zhou1Caixia Liu2Jing Zhang3Ning Li4Zhanli Zhao5Guoyong Sun6Yaohua Zhong7State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong UniversityShandong Xingguang Sugar Group Co. Ltd.Shandong Academy of Pharmaceutical SciencesState Key Laboratory of Microbial Technology, School of Life Sciences, Shandong UniversityShandong Xingguang Sugar Group Co. Ltd.Shandong Xingguang Sugar Group Co. Ltd.Anaesthesiology Department of the Second Hospital of Shandong UniversityState Key Laboratory of Microbial Technology, School of Life Sciences, Shandong UniversityAbstract The industrial microorganisms used for fructooligosaccharides (FOS) synthesis are generally fermented with sucrose as carbon source to induce the production of β-fructofuranosidase (FFase) having transfructosylation activity. Consequently, isolation of novel FFase producers from a sucrose-enriched biotope would help improve FOS productivity and reduce the process cost. Here, three fungi isolated from a unique sugarcane molasses habitat were found to possess FFase activity and one of them, XG21, exhibited a high capacity to synthesize FOS. Analysis of its morphological properties and ribosomal internal transcribed spacer (ITS) sequence allowed the taxonomic position to be assigned and it was thus identified as Aspergillus tubingensis XG21. It could utilize various potential carbon sources for vigorous growth, but only produced high-level FFase activity on sucrose. Furthermore, the transfructosylation ability and FOS synthesis were analyzed by TLC and HPLC. During the transfructosylation reaction, an increase in sucrose concentration led to the remarkable enhancement in FOS formation with the maximum content of up to 56.9% within 8 h. Finally, the sugarcane molasses was used to cultivate A. tubingensis XG21 and the optimal FFase activity reached up to 558.3 U/g, which was 88.9% higher than that with sucrose as carbon source. These results indicate that A. tubingensis XG21 can be considered as a new genetic resource adapted to cheaply available carbon sources for FOS production.http://link.springer.com/article/10.1186/s13568-017-0428-8Fructooligosaccharides productionβ-fructofuranosidaseAspergillus tubingensisSugarcane molasses |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yijia Xie Huanxia Zhou Caixia Liu Jing Zhang Ning Li Zhanli Zhao Guoyong Sun Yaohua Zhong |
spellingShingle |
Yijia Xie Huanxia Zhou Caixia Liu Jing Zhang Ning Li Zhanli Zhao Guoyong Sun Yaohua Zhong A molasses habitat-derived fungus Aspergillus tubingensis XG21 with high β-fructofuranosidase activity and its potential use for fructooligosaccharides production AMB Express Fructooligosaccharides production β-fructofuranosidase Aspergillus tubingensis Sugarcane molasses |
author_facet |
Yijia Xie Huanxia Zhou Caixia Liu Jing Zhang Ning Li Zhanli Zhao Guoyong Sun Yaohua Zhong |
author_sort |
Yijia Xie |
title |
A molasses habitat-derived fungus Aspergillus tubingensis XG21 with high β-fructofuranosidase activity and its potential use for fructooligosaccharides production |
title_short |
A molasses habitat-derived fungus Aspergillus tubingensis XG21 with high β-fructofuranosidase activity and its potential use for fructooligosaccharides production |
title_full |
A molasses habitat-derived fungus Aspergillus tubingensis XG21 with high β-fructofuranosidase activity and its potential use for fructooligosaccharides production |
title_fullStr |
A molasses habitat-derived fungus Aspergillus tubingensis XG21 with high β-fructofuranosidase activity and its potential use for fructooligosaccharides production |
title_full_unstemmed |
A molasses habitat-derived fungus Aspergillus tubingensis XG21 with high β-fructofuranosidase activity and its potential use for fructooligosaccharides production |
title_sort |
molasses habitat-derived fungus aspergillus tubingensis xg21 with high β-fructofuranosidase activity and its potential use for fructooligosaccharides production |
publisher |
SpringerOpen |
series |
AMB Express |
issn |
2191-0855 |
publishDate |
2017-06-01 |
description |
Abstract The industrial microorganisms used for fructooligosaccharides (FOS) synthesis are generally fermented with sucrose as carbon source to induce the production of β-fructofuranosidase (FFase) having transfructosylation activity. Consequently, isolation of novel FFase producers from a sucrose-enriched biotope would help improve FOS productivity and reduce the process cost. Here, three fungi isolated from a unique sugarcane molasses habitat were found to possess FFase activity and one of them, XG21, exhibited a high capacity to synthesize FOS. Analysis of its morphological properties and ribosomal internal transcribed spacer (ITS) sequence allowed the taxonomic position to be assigned and it was thus identified as Aspergillus tubingensis XG21. It could utilize various potential carbon sources for vigorous growth, but only produced high-level FFase activity on sucrose. Furthermore, the transfructosylation ability and FOS synthesis were analyzed by TLC and HPLC. During the transfructosylation reaction, an increase in sucrose concentration led to the remarkable enhancement in FOS formation with the maximum content of up to 56.9% within 8 h. Finally, the sugarcane molasses was used to cultivate A. tubingensis XG21 and the optimal FFase activity reached up to 558.3 U/g, which was 88.9% higher than that with sucrose as carbon source. These results indicate that A. tubingensis XG21 can be considered as a new genetic resource adapted to cheaply available carbon sources for FOS production. |
topic |
Fructooligosaccharides production β-fructofuranosidase Aspergillus tubingensis Sugarcane molasses |
url |
http://link.springer.com/article/10.1186/s13568-017-0428-8 |
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