Focal adhesion kinase is required for actin polymerization and remodeling of the cytoskeleton during sperm capacitation
Several focal adhesion proteins are known to cooperate with integrins to link the extracellular matrix to the actin cytoskeleton; as a result, many intracellular signaling pathways are activated and several focal adhesion complexes are formed. However, how these proteins function in mammalian sperma...
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2016-09-01
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doaj-c7b8601bed0644a39f8d7c0f122dc1362021-06-02T15:44:33ZengThe Company of BiologistsBiology Open2046-63902016-09-01591189119910.1242/bio.017558017558Focal adhesion kinase is required for actin polymerization and remodeling of the cytoskeleton during sperm capacitationAna L. Roa-Espitia0Eva R. Hernández-Rendón1Rafael Baltiérrez-Hoyos2Rafaela J. Muñoz-Gotera3Antonieta Cote-Vélez4Irma Jiménez5Humberto González-Márquez6Enrique O. Hernández-González7 Departamento de Biología Celular, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, México D.F. 07360, México Departamento de Biología Celular, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, México D.F. 07360, México Universidad Autónoma Benito Juárez de Oaxaca, Facultad de Medicina y Cirugía, Oaxaca, Oaxaca 68120, México Facultad de Ciencias Veterinarias, Universidad de Zulia, Maracaibo 4012, Venezuela Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México 62210, Cuernavaca, México Posgrado en Biología Experimental, Universidad Autónoma Metropolitana-Iztapalapa, México D.F. 09349, México Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, México D.F. 09349, México Departamento de Biología Celular, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, México D.F. 07360, México Several focal adhesion proteins are known to cooperate with integrins to link the extracellular matrix to the actin cytoskeleton; as a result, many intracellular signaling pathways are activated and several focal adhesion complexes are formed. However, how these proteins function in mammalian spermatozoa remains unknown. We confirm the presence of focal adhesion proteins in guinea pig spermatozoa, and we explore their role during capacitation and the acrosome reaction, and their relationship with the actin cytoskeleton. Our results suggest the presence of a focal adhesion complex formed by β1-integrin, focal adhesion kinase (FAK), paxillin, vinculin, talin, and α-actinin in the acrosomal region. Inhibition of FAK during capacitation affected the protein tyrosine phosphorylation associated with capacitation that occurs within the first few minutes of capacitation, which caused the acrosome reaction to become increasingly Ca2+ dependent and inhibited the polymerization of actin. The integration of vinculin and talin into the complex, and the activation of FAK and paxillin during capacitation, suggests that the complex assembles at this time. We identify that vinculin and α-actinin increase their interaction with F-actin while it remodels during capacitation, and that during capacitation focal adhesion complexes are structured. FAK contributes to acrosome integrity, likely by regulating the polymerization and the remodeling of the actin cytoskeleton.http://bio.biologists.org/content/5/9/1189Acrosome reactionCapacitationCytoskeletonProtein tyrosine (Tyr) phosphorylationCalcium PyK2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana L. Roa-Espitia Eva R. Hernández-Rendón Rafael Baltiérrez-Hoyos Rafaela J. Muñoz-Gotera Antonieta Cote-Vélez Irma Jiménez Humberto González-Márquez Enrique O. Hernández-González |
spellingShingle |
Ana L. Roa-Espitia Eva R. Hernández-Rendón Rafael Baltiérrez-Hoyos Rafaela J. Muñoz-Gotera Antonieta Cote-Vélez Irma Jiménez Humberto González-Márquez Enrique O. Hernández-González Focal adhesion kinase is required for actin polymerization and remodeling of the cytoskeleton during sperm capacitation Biology Open Acrosome reaction Capacitation Cytoskeleton Protein tyrosine (Tyr) phosphorylation Calcium PyK2 |
author_facet |
Ana L. Roa-Espitia Eva R. Hernández-Rendón Rafael Baltiérrez-Hoyos Rafaela J. Muñoz-Gotera Antonieta Cote-Vélez Irma Jiménez Humberto González-Márquez Enrique O. Hernández-González |
author_sort |
Ana L. Roa-Espitia |
title |
Focal adhesion kinase is required for actin polymerization and remodeling of the cytoskeleton during sperm capacitation |
title_short |
Focal adhesion kinase is required for actin polymerization and remodeling of the cytoskeleton during sperm capacitation |
title_full |
Focal adhesion kinase is required for actin polymerization and remodeling of the cytoskeleton during sperm capacitation |
title_fullStr |
Focal adhesion kinase is required for actin polymerization and remodeling of the cytoskeleton during sperm capacitation |
title_full_unstemmed |
Focal adhesion kinase is required for actin polymerization and remodeling of the cytoskeleton during sperm capacitation |
title_sort |
focal adhesion kinase is required for actin polymerization and remodeling of the cytoskeleton during sperm capacitation |
publisher |
The Company of Biologists |
series |
Biology Open |
issn |
2046-6390 |
publishDate |
2016-09-01 |
description |
Several focal adhesion proteins are known to cooperate with integrins to link the extracellular matrix to the actin cytoskeleton; as a result, many intracellular signaling pathways are activated and several focal adhesion complexes are formed. However, how these proteins function in mammalian spermatozoa remains unknown. We confirm the presence of focal adhesion proteins in guinea pig spermatozoa, and we explore their role during capacitation and the acrosome reaction, and their relationship with the actin cytoskeleton. Our results suggest the presence of a focal adhesion complex formed by β1-integrin, focal adhesion kinase (FAK), paxillin, vinculin, talin, and α-actinin in the acrosomal region. Inhibition of FAK during capacitation affected the protein tyrosine phosphorylation associated with capacitation that occurs within the first few minutes of capacitation, which caused the acrosome reaction to become increasingly Ca2+ dependent and inhibited the polymerization of actin. The integration of vinculin and talin into the complex, and the activation of FAK and paxillin during capacitation, suggests that the complex assembles at this time. We identify that vinculin and α-actinin increase their interaction with F-actin while it remodels during capacitation, and that during capacitation focal adhesion complexes are structured. FAK contributes to acrosome integrity, likely by regulating the polymerization and the remodeling of the actin cytoskeleton. |
topic |
Acrosome reaction Capacitation Cytoskeleton Protein tyrosine (Tyr) phosphorylation Calcium PyK2 |
url |
http://bio.biologists.org/content/5/9/1189 |
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